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AULA 10

1. Síntese de ácidos gordos

2. Síntese de colesterol

3. Regulação hormonal

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Fontes de ácidos gordos

glucose

piruvato

gliceraldeído-3-Pi

acetil-CoA

malonil-CoA

palmitoil-CoA

acetil-CoA

piruvatoc.citrato

Sintetasede ácidos

gordos

Acetil-CoA

carboxilase

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P citrato

citrato

P

parcialmenteactiva

activa inactiva

PATP ADP

H2O

Proteina cinase AMPdependente

Proteína fosfatase 2A

Regulação da acetil-CoA carboxilase

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AMP-cinase

Cataliza a fosforilação da Acetil-CoA carboxilase <> INACTIVAÇÃO

É activada por AMP <> baixa carga energética

paragem da síntese de ác.gordos

Ocorre ββββ-oxidação de ác.gordos

Aumento da conc. de ATP inibição da AMP-cinase

recomeço da síntese de ác.gordos

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Biosíntese do colestrol

Etapa controladora

C2 C4 C6 C6

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A etapa controladora depende de NADPH produzido

quer no ciclo do citrato que na via das pentose-fosfato

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Structure of the catalytic portion of human HMG-CoA Reductase.Determined by E. S. Istvan, M. Palnitkar, S. K. Buchanan, & J. Deisenhofer in 1999

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Síntese do lanosterol

esqualeno mono-oxigenase

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Como ocorrem as 3 desmetilações ?

a) Via oxidação com consumo de O2 / sistema Cit.P-450b) Formação de um carboxilo

c) Descarboxilação

citocromo P-450

Structure of the hemoproteinDomain ofcytochrome P450 from Bacillusmagaterium,

by H. Li & T. L. Poulos in 1994

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CH3

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Julio Resende

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