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AULA 10
1. Síntese de ácidos gordos
2. Síntese de colesterol
3. Regulação hormonal
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Fontes de ácidos gordos
glucose
piruvato
gliceraldeído-3-Pi
acetil-CoA
malonil-CoA
palmitoil-CoA
acetil-CoA
piruvatoc.citrato
Sintetasede ácidos
gordos
Acetil-CoA
carboxilase
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P citrato
citrato
P
parcialmenteactiva
activa inactiva
PATP ADP
H2O
Proteina cinase AMPdependente
Proteína fosfatase 2A
Regulação da acetil-CoA carboxilase
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AMP-cinase
Cataliza a fosforilação da Acetil-CoA carboxilase <> INACTIVAÇÃO
É activada por AMP <> baixa carga energética
paragem da síntese de ác.gordos
Ocorre ββββ-oxidação de ác.gordos
Aumento da conc. de ATP inibição da AMP-cinase
recomeço da síntese de ác.gordos
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Biosíntese do colestrol
Etapa controladora
C2 C4 C6 C6
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A etapa controladora depende de NADPH produzido
quer no ciclo do citrato que na via das pentose-fosfato
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Structure of the catalytic portion of human HMG-CoA Reductase.Determined by E. S. Istvan, M. Palnitkar, S. K. Buchanan, & J. Deisenhofer in 1999
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Síntese do lanosterol
esqualeno mono-oxigenase
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Como ocorrem as 3 desmetilações ?
a) Via oxidação com consumo de O2 / sistema Cit.P-450b) Formação de um carboxilo
c) Descarboxilação
citocromo P-450
Structure of the hemoproteinDomain ofcytochrome P450 from Bacillusmagaterium,
by H. Li & T. L. Poulos in 1994
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CH3
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Julio Resende
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