Carolina Jaramillo Mesa

Medical student UPB

ID: 00012039

Molecular biology

August 6 2012

Recognition of ORI (Originof replication)

Helicase

DNA unroll

DNA polymerase

DNA replicateReparation of

DNA

Mutation

Conservation and transmition of the DNA

Trypanosoma brucei it’s a parasite type

kinetoplastia, wich have the mtDNA encapsulated in

the mithocondrion with many copies of the

mitochondrial genome. This parasite cause African

sleeping sickness.

Taken from: http://www.microbiologyonline.org.uk/about-

microbiology/introducing-microbes/protozoa

Taken from:

http://www.parasitemuseum.com/trypanosome/

DNA circlesDNA

polymeraseCopy the

information

Daughter

cells

Relationship withreplicating kDNA

molecules

ExperimentInmunofluorescence

DNA polymerase ID

Localization of DNA polymerase ID

This parasites cant be eradicate with antibiotics by the

complex mitochondrial genome and the only way to kill

them is using toxics. This research proposed a alternative

treatments more effectivs and less agresives.

Meier-Gorlin

syndrome : it is

characterized for

severe growth

defects, skeletal

abnormalities and

small brain size.

Taken from

http://abbottgenetics.wordpress.com/2009/03/09/

why-the-littlest-angel-was-so-little-by-bryan-cooke/

ORC recognize the ORI to start the DNA

replication and coordinate that the

genetic material duplicate just once. Also

ensure the correct duplication of the

genetic material through centrosome.

Taken from: http://www.intechopen.com/books/dna-replication-current-

advances/the-silencing-face-of-dna-replication-gene-repression-mediated-by-

dna-replication-factors

pre-RC recognize theORI

DNA replicaction

Regulation of thecentrosome

Orc1-encoding gene is mutated

Reduplication of the centrosome

alteration of the cell cycle and cell signaling

Knowing the causes of the disease allow us

treat this mutation before its expression,

through genetic therapy with the objective

to prevent the damage and in a future

decrease the number of kids with Meier

Gorlin syndrome.

Taken from: http://www.fedaes.es/FOLLFA/B14-TERA.htm

This research expose us alternative

treatments to diseases as complicated as

those caused by parasites, African sleeping

sickness, Chagas disease and leishmaniasis

and understand the cellular processes that

take place in these diseases.

The genetic diseases have increased with the

pass of the years, they are misunderstood

because the cause of them are unknown in the

most part of cases and the treatment are very

limited, so as a doctors is very important inform

about this diseases and exposed our patients the

alternatives treatments and the explain them all

about this diseases.

Taken from:

http://www.sitiosespana.com/notas/2010/mayo/oligof

renia.htm

MARTINEZ, Lina; Biologia molecular, Vol 6.

ed. UPB, 2011. Paginas 74-81.

Secrets of Parasites' Replication Unraveled;

Sciencedaily; july 9 2012;

http://www.sciencedaily.com/releases/2012

/07/120710141940.htm

Protein Involved in DNA Replication,

Centrosome Regulation Linked to Dwarfism,

Small Brain Size; Sciencedaily; July 31 2012;

http://www.sciencedaily.com/releases/2012

/07/120731200628.htm