emka TECHNOLOGIES pulmonary.emka.fr

pulmonary solutions for small & large animals

pulmonary solutions from emka TECHNOLOGIES

emka TECHNOLOGIES

pulmonary solutions from emka TECHNOLOGIES

» iox2 acquires, analyzes, displays, controls and stores data generated during an experiment

» one file per subject (can append data to same file during successive sessions)

» subject file contains all information (configuration, calibration, protocol, event and signal data)

» real-time monitoring, on line or off line review » synchronized video signal stored in same file or in separate file

(user option) » protocols as experimental guideline or automation » remote control of nebulization

studyDESIGNER is a high level tool to organize data recording, analysis, review and archiving on large toxicology or safety pharmacology studies.

During recording sessions, studyDESIGNER automatically drives iox2, ecgAUTO and datanalyst software to record and analyze data and send calculated parameters to its data base.

» analysis module for respiration from conductive belts » synchronized video review » pioneering shape-recognition techniques for in-depth analysis of ECG for all species, normal and pathological morphologies

» defines subject and study structure » defines recording protocols and analysis configurations » reviews and edits analysis results » stores and archives results in database » generates on-demand/custom reports for any combination

of group, phase, subject and all parameters

software suite for all your needs

Available for all software:

» electronic signature » audit trail » user access management » study management

service & support

emka TECHNOLOGIES provides a full range of support services:

» pre-installation audits to design optimal system setup, SOP

» installation and training » hardware and software validation service » warranty extension and maintenance

datanalyst processes calculated parameters from files generated by iox2. Fast and versatile data from an unlimited number of experiments are extracted automatically.

Extraction of key data

» edits event markers, finds min, max, area, kinetics... » automated extraction using protocols » results as graphs & tables

Data reduction

» pools subjects into single study » full experiment review, identifies protocol events » exclusion of artefacts

Reporting

» distributes subjects in groups » performs simple statistics, computes EC50, kinetics... » versatile text format export

Specific analyzers for real time processing of:

» respiratory flow, penh, cough and apnea detection » lung resistance/compliance

ecgAUTO, advanced post-processing analysis

datanalyst, data post-processing software

iox2, data acquisition and real time analysis

studyDESIGNER, data base tool for your studies

GLP modules

emka TECHNOLOGIES

pulmonary solutions from emka TECHNOLOGIES

» defines subject and study structure » defines recording protocols and analysis configurations » reviews and edits analysis results » stores and archives results in database » generates on-demand/custom reports for any combination

of group, phase, subject and all parameters

» for mouse, rat, guinea-pig, rabbit, primate » regulated ventilation for noise-free air renewal » drinking bottle for long experiments (24 hrs or more)

» tower for swivel/tether system, for IV injection or blood pressure » temperature and humidity monitoring and compensation » behavioral and EEG studies in rats. Height design ensures that the

rat can stand on its hind legs into the plethysmograph

» respiratory rates and durations » indication on breathing depth, volume and flow » quantifies the degree of bronchoconstriction (penH)

The non-invasive whole body plethysmography is a proven way to study pulmonary function and evaluate bronchoconstriction on unrestrained conscious animals.

» moving freely, animals are neither stressed, nor influenced by anesthesia

» same subjects can be their own control and be studied several times

» start experiment on multiple animals only in a few minutes

Key features

Options

Measured parameters

» measures respiratory parameters (like in whole body plethysmograph model)

» gets complete 6-lead ECG signal

» designed for conscious restrained mouse and rat

» usually performed within whole body plethysmograph, sometimes in double chamber plethysmograh

» audio and respiration signals are correlated to reject random sound noise : only true cough bursts are processed

» optional video recording helps performing quick control

The double chamber plethysmograph measures nasal flow from the head chamber and thoracic flow from the body chamber.

» computes specific airway resistance using the time shift between nasal and thoracic flows

» measures ventilatory parameters on mouse, rat, guinea-pig » conical restrainer for less stressed animal and easier manipulation » aerosol can be delivered in head chamber » the head-out configuration (head part is removed) can be

combined with inhalation tower to expose several animals simultaneously, keeping them under measurement (all ventilatory parameters are measured except specific airway resistance)

Conscious restrained animals can be used for long-term measurements and chronic studies. A large number of subjects can be screened, with no surgery, nor anesthetic.

emka TECHNOLOGIES provides a complete suite of integrated solutions for pulmonary studies:

» conscious, unrestrained, freely moving animal in whole body plethysmograph

» conscious, restrained animal in head-out or double chamber plethysmograph

» anesthetized animal in standard plethysmograph, with spontaneous or ventilated breathing

» full pulmonary mechanics systems

» exposure systems : aerosol, gas, cigarette smoke

Combining the lung pressure signal coming from a telemetry implant with flow signal from a double chamber plethysmograph in the head-out configuration allows direct resistance/compliance measurements in non-sedated animals.

ecgTUNNEL

whole body plethysmograph

double-chamber plethysmography & implantable telemetry

double chamber & head-out plethysmograph

combined measurement of respiration & ECG

cough detection

New

easyMATRIX

Take advantage of the emka TECHNOLOGIES software suite with the easyMATRIX16, which synchronizes respiratory signal & pressure signal from DSITM implant.

» broadest range of measurements in a single platform » volume or pressure-controlled mechanical ventilation » customized or standard pressure or volume manoeuvers » dynamic R&C measurements » low frequency forced oscillations » unique distinction between central & peripheral lung mechanics » animals from 10 g to 40 kg » pressure-volume loop with static compliance » automatic Control of Peep » advanced µCT & µMRI lung gating » enhanced drug delivery system » dose/response curves » files exported under Excel® or text

On anesthetized animals, pulmonary pressure and flow are measured.

This allows to access lung resistance and compliance and, as well, full list of ventilation parameters.

Mainly used on ventilated animals, this models is also used with spontaneously breathing subjects.

For large animals, plethysmographs can not be used and are replaced by heated Fleisch tube measuring flow. On anesthetized animals, pressure is measured from pleura or oesophagus.

» for mouse, rat and guinea-pig » tracheotomized or intubated animals » thoracic flow is computed from pressure changes in the plethysmograph » pressure is measured from a cannula inserted in the pleural, oesophagus or trachea » can be combined with vital signs monitoring » aerosol can be delivered during ventilation

complete pulmonary mechanics on rodents

FEV extension

flexiVentTM

MSX Multi-Subject extension

» FEV extension for FEVx, FVC and PEF measurements: flexiVent can uniquely perform both forced expiratory and forced oscillation measurements.

» Multi-Subject extention, for measurement in parallel subjects (1 to 4 subjects)

Options

large animals with Fleish tubes

plethysmograph for anesthetized rodents

advanced respiratory mechanics with flexiVentTM

pulmonary.emka.fr

respiratory parameters from large animals

Lung volume measurement via respiratory inductive plethysmography (RIP) is a proven method for quantitative and qualitative non-invasive respiratory measurements on large animals:

» for freely moving primate, dog, pig etc. » thoracic and/or abdominal belts are fully adjustable and directly connected to emkaPACK4G transmitter » emkaPACK4G transmitter is housed into a jacket, worn by the animal » data is wirelessly transmitted by telemetry » respiration flow is derived from lung volume

» all standard pulmonary parameters » phase shift computation for bronchoconstriction assessment » easy subject specific absolute calibration » ECG, blood pressure, acceleration & temperature can be optionally acquired,

simultaneously with respiration

exposure systemsaerosol generation and delivery

» generation rate adjusted from 5% to 100% of maximum delivery » continuous nebulization or only during inspiration » nebulization started manually or by iox2 software » large variety of nebulization protocols automatically executed » drives up to 4 Aerogen Aeroneb® nebulizers

» multiple controllers can be linked to drive more than 4 Aeroneb® nebulizers

mass flow controller

exposure to cigarette smoke and toxins

Ask for specific brochure and see all our telemetry solutions on telemetry.emka.fr

inExposeTM for exposing laboratory subjects to cigarette smoke, aerosolized liquids, solutions, suspensions, inhaled toxins, compounds, vapours, gases... :

» controlled delivery of multiple gas/toxin mixtures

» nose-only and whole-body exposure

» cigarette smoke exposure for mice and rats: . computer-controlled & programmable rotary cigarette smoker . up to 24 cigarettes smoked in any possible parallel/sequential combination, with programmable puffing profiles and automatic lighting & ejection

» option for plethysmography » deliver a specific gas mixture » ideal to evaluate the effects of hypoxia, hyperoxia, hypercapnia etc.

» aquisition is performed in iox2 software which also controls the mass flow controllers

» modify the composition of the gas mixture in real time, directly from iox2 software

Precise and automated aerosol delivery with Aerogen Aeroneb® nebulizer, emka TECHNOLOGIES’ nebulizationCONTROLLER and iox2 software:

emkaPACK4G transmitter with RIP belts

cigarette smoking robotinExposeTM

RIP respiration from belts

nebulizationCONTROLLER and plethysmograph with Aerogen Aeroneb® nebulizer

emka TECHNOLOGIES

pulmonary solutions from emka TECHNOLOGIES

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CONTACT US!for simple to very demanding projects, please call on us

europe emka TECHNOLOGIES SA59, bd. Général Martial Valin - 75015 Paris - France tel: +33 (0)1 40 60 76 00 - fax: +33 (0)1 40 60 65 55

emka@emka.fr

north america emka TECHNOLOGIES Inc.307 Annandale Road, suite 203 - Falls Church, VA 22042 - USA tel: +1 (703)237-9001 - fax: +1 (703)237-9006

emkatech@emkatech.com

chinaBeijing GYD Labtech Co Ltd Rm 935 Laho Studio, No.15-A1 East Jianguo Road Chaoyang District, Beijing 100024 tel: +86-(0)10-85376382 or +86-(0)13-031151180 or +86-(0)13-01151181fax:+86-(0)10-58208944 info@bjgyd.com

Our systems include all needed hardware and software components. A one stop shop solution to be sure to obtain a reliable, field proven setup.

4 compelling reasons to choose emka TECHNOLOGIES pulmonary systems!

Cough detection, temperature and humidity correction, combined telemetry measurement, automated aerosol generation, synchronized video, connection to LIMS and many more solutions to answer your specific study requirements. Do not compromise; challenge us with tough questions!

wide array of options, solutions & add-ons

complete solution for pulmonary studies

20 years of experience

21 cfr part 11 compliance

emka TECHNOLGIES has been providing in depth pulmonary study systems from its start in 1991.

Our list of references includes more than 300 users worldwide. We produce single files per subject which not only contain the raw data and the calculated data points, but also the complete copy of all settings, calibration values etc. This is a major advantage when having to comply with 21 cfr part.

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