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Toxicological Risk Assessment

An initiative of

24 October 2013 - UCL - Brussels (Woluwe)

Namur Nanosafety Centre

University of Namur

Dr Christelle Saout Senior scientist

Pr Stéphane Lucas

Director of NARILIS

Fields of expertise

• Nanomaterial (NM) physicochemical characterisation:

pristine forms, dispersions, complex matrices

• NM fate and biodistribution studies at cellular and organ levels

• Assessment of NM potential toxicity: in vitro & in vivo testing

following OECD guidelines

• Metrology, cross validation studies for regulatory purposes:

validated supplier of EC-JRC IRMM

• Animal whole-body exposure to standardized airborne

aerosols: local and systemic impacts, biopersistence

• Heamocompatibility of NMs

• Toxicity mechanistic studies

• Nanomaterial regulation: OECD WPMN, EC DG Consumers &

Health, GAARN- European Chemical Agency, BE Superior Health

council 3

Fields of expertise

Industrial applications fields Potentially, all industrial sectors involved nanotechnologies

• NM manufacturers

• Paint & coating

• Construction

• Transport

• Cosmetic

• Food

• Biomedical and health

• Electronics

• Aerospace

•…

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Industrial targets

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Applied research projects in Nanotoxicology

Brussels – Oct 24, 2013

QualityNano : A pan-European Infrastructure Project for quality in nanomaterials safety testing. Joint Research and Networking Activities and provision of Transnational Access. (7 M€, 2011-14, INFRASTRUCTURE-2010-1-262163). http://www.qualitynano.eu

NANOVALID: Large scale integrated project: Development of reference methods for hazard identification, risk assessment and LCA of engineered nanomaterials (7 M€, 2011-14, NMP4-LA-2011-263147). http://www.nanovalid.eu

NANoREG: Large scale integrated project: Regulory testing of Nanotechnology The approach of NANoREG will provide answers to Society, Industry and the National Regulation and Legislation Authorities (48 M€, 2013-16, NMP4-LA-2013, 310584). http://www.nanoreg.eu

Ongoing projects

Program of Excellence NANOTOXICO n°516252: Coordinator : Unamur–(Jan 2006 to Dec 2012) – Total Budget 10 M€ co-sponsored by DGO6-SPW & Unamur.

NANOPOLYMERES project: n°DG5/MR-RB/2007/nano1. Coordinator: ISP- Nov. 2007 to June 2008) – Total Budget : 32.000,00 €.

Silicalloy project: Convention n°6144 MECATECH Pole. Coordinator : De LEUZE s.a. –Jan. 2009 to June 2013)–Total Budget 1.2 M€

Closed projects

NM physicochemical characterisation

• 2 MeV Tandetron linear accelerator (Altaïs) for NM-core chemical characterisation

• Field Emission Gun - Scanning Electron Microscope JSM-

7500F /Jeol (resolution 0.6 nm) with EDX detector for morphology and global chemical composition

• CPS 24000 Disc Centrifuge for nanoparticle size analysis

• BET for porosity evaluation

• XRD for phase and crystallinity evaluation

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Special equipments

NM toxicity assessment In vivo/ ex vivo testing

• Impact-R®, PFA-100®, light transmission aggregometry, electron microscopy, KC-10®, cTGT for NM impact on blood

• Whole body inhalation exposure models for biodistribution and biopersistence studies

In vitro testing

• Cell culture platforms – Tissue engineering - Young vs Elderly • Confocal microscopy (Leica TCS SP5) for cellular uptake and

DNA damage studies • Gene expression profiling and proteomic platforms for

toxicity screening

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Special equipements

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Adapted to the demand

• Physicochemical characterisation: size distribution, shape,

surface properties (pristine form, complex matrices)

• NM release studies – Identification of potential exposure

• NM solubility

• NM transport /crossing through biological barriers

• Toxicity screening testings – safe by design strategy

• …

Services provided to companies

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Training proposals to companies

ITEMS

• Selection of best and most appropriate analytical technique for

NM investigation

• Training about in vivo and in vitro toxicity assessment

• On site NM worker exposure measurements

• Best NM manipulation practice

• Origin of NM generation as byproduct for vacuum based

technologies

• …

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Contacts

Pr Stéphane Lucas Director of Narilis– Characterisation studies stephane.lucas@unamur.be +32 81 72 55 28 or (54 81) Dr Christelle Saout Senior scientist– in vitro studies christelle.saout@unamur.be 32 81 72 57 13 Dr Olivier Toussaint Senior FNRS research associate – In vitro studies olivier.toussaint@unamur.be +32 81 21 54 87 Pr Jean-Michel Dogné Director of the Pharmacy Dpt.– In vivo and hemocompatibility studies jean-michel.dogne@unamur.be +32 81 72 57 13

University of Namur Namur Nanosafety Centre

Rue de Bruxelles, 61 5000 Namur

http://www.narilis.be/technological-facilities/namur-nanosafety-center