Ion TorrentThe Chip is the Machine

scalabilitysimplicityspeed

Ion TorrentThe Chip is the Machine™

Discover Ion TorrentThe Ion Personal Genome Machine™ (PGM™) is simpler, more cost-effective, and more scalable than any other sequencing technology. The PGM™ sequencer is a benchtop system utilizing groundbreaking and disruptive semiconductor technology that enables rapid and scalable sequencing experiments.

Ion Torrent technology uses a massively parallel array of proprietary semiconductor sensors to perform direct, real-time measurement of the hydrogen ions produced during DNA replication. A high-density array of wells on the Ion semicon-ductor chips provides millions of individual reactors, and integrated fluidics allow reagents to flow over the sensor array. This unique combination of fluidics, micromachining, and semiconductor technology enables the direct translation of genetic information (DNA) to digital information (DNA sequence), rapidly generating large quantities of high-quality data.

The Personal Genome Machine™, along with Ion Torrent semiconductor chips, Ion Torrent reagent kits, and the Torrent Server/Torrent Suite software, allow Ion Torrent to deliver a cutting-edge sequencing solution.

Scalabilitymassively scalable semiconductor technology

Simplicitynatural biochemistry, no optical components

Speedruns in about two hours, not days or weeks

Discover scalabilityscales like a semiconductor

• Leverages $1 trillion semiconductor industry investment

• Scales by density: 40 cumulative years of Moore’s Law

• Scales by volume: 400 standard semiconductor foundries from which to choose

Discover simplicitysimple chemistry

• Simplest sequencing chemistry: hydrogen ion detection (pH meter)

• Natural nucleotides: no enzymatic cascade, no fluorescence, and no chemiluminescence

• Low-cost, standard reagents

Discover speeddirect, real-time detection technology

• Makes sequencing accessible to new applications and markets

• Short run time supports fast research cycle and faster time to publication

• The Chip is the Machine™: no optics, no light, no scanning

Sequencing and analysis pipeline

The Ion Torrent™ sequencing workflow consists of a number of standard elements—creation of a DNA or RNA library, a simple PCR process creating amplified templates on proprietary Ion Sphere™ particles, followed by a sequenc-ing run on the PGM™ platform.

With the completion of a PGM™ sequencer run, data automatically transfer to the dedicated Torrent Server that runs Torrent Suite software. Here, raw Ion signals are converted to base calls and stored in industry-standard SFF or FASTQ files. These data can be processed by a variety of commercially available software packages for applications such as variant detection, RNA-Seq, ChIP-Seq, or de novo genome assembly.

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For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.© 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Printed in the USA. CO23490 0711

Ion 314™ Chip

Scale 1.3 million wells

Data output Starting at 10 Mb of high-quality sequence*

Ion 316™ Chip

Scale 6.3 million wells

Data output Starting at 100 Mb of high-quality sequence*

Ion 318™ Chip†

Scale ~12 million wells

Data output Starting at 1 Gb of high-quality sequence*

For all chips

Data output SFF/FASTQ file output

Reagents Complete sets of reagents, including library, template, and sequencing kits

Applications Amplicon sequencing, microbial sequencing, RNA-Seq, ChIP-Seq, targeted resequencing, copy number analysis, methylation, paired-ends

Specifications

Ordering informationDescription Part No.

Personal Genome Machine™

Ion PGM™ System 4462921

Includes PGM™ sequencer (4462917) and Torrent Server (4462918)

Service Contracts

AB Assurance Plan ZG11SCIONPGMSYS

Rapid Exchange Program AB Assurance Plan ZGEXSCIONPGMSYS

Semiconductor Sequencing Chips

Ion 314™ Chip Kit (8 chips) 4462923

Ion 316™ Chip Kit (4 chips) 4466616

Ion 316™ Chip Kit (8 chips) 4469496

Reagent Kits

Ion Xpress™ Fragment Library Kit (up to 20 reactions) 4468987

Ion Fragment Library Kit (up to 20 reactions) 4466464

Ion Total RNA-Seq Kit (12 reactions) 4466666

Ion DNA Barcode 1-16 Kit† (10 sets of 16 libraries) 4468654

Ion Xpress™ Template Kit (10 reactions) 4469001

Ion Sequencing Kit (8 reactions) 4468997

Ion Control Kits

Ion Control Materials Kit (3 reactions each per control) 4466465

Ion Sphere™ Quality Control Kit (20 measurements) 4468656

Ion Library Quantitation Kit (250 reactions) 4468802* Throughput calculated with Escherichia coli DH10B genome, using Ion Sequencing 314 chemistry and a 100 bp read length PGM™ sequencer configuration. † The content provided herein may relate to products that have not been officially released and is subject to change without notice.