Honolulu Cloning Technique

By: Ashley Boggs

What is the Honolulu Cloning Technique?

A technique that uses adult cells and nuclei for cloningUsed to clone three generations of miceFirst clone was known as Cumulina

Honolulu Cloning

First used in July of 1998Experiment was at the University of HawaiiDeveloped by Ryuzo Yanagimachi and Teruhiko Wakayama

Steps of the Honolulu Technique

1. The cell’s nucleus is removed2. A donor nucleus is injected into an

egg3. The new cell is chemically cultured

to jumpstart cell growth

Steps of the Honolulu Technique Continued

4. This created embryos5. Which are placed

into the surrogate mothers to be carried

The method is repeated over and over to create 2nd and 3rd generations of identical mice

Pros of the Technique

Used to create clones, exact copies; genetic replicasHad very successful nuclei used, cumulus cellsMore successful cloning rate than the Rosalin techniqueCan help scientists do further research on egg reprogramming in a nucleus

Cons of Cloning

It was a process of trial and error, so before there were good clones there were many mistakes and mutations madeReduces genetic diversityExpensiveOne virus could knock out an entire species of clones because of a lack of genetic diversity

Uses of Honolulu Cloning

To further cell researchTo produce transgenic animalsTo produce drugs from transgenic animalsUse clones to “evaluate molecular mechanisms”Critique current techniques and create better ones

The End.