Review of slides in

mycology

Pictures c/0 Karentan.Content c/o chumtemp. OLFU Med II 2008

Dermatophytosis/cutaneous mycoses

“Tineas”

FUNGIFACTS: DERMATOPHYTESGenus Species Macroconidia Microconidia Disease(s) Produced

Epidermophyton floccosum Numerous Smooth- walled

Absent Tinea corporis, pedis, ungium, cruris

Microsporum canis Numerousthick walledRoughPOINTED ENDS(+)Spiny tips

Rare Tinea capitisTinea corporis

Microsporum gypseum NumerousthiN walledRoughBLUNT ENDS

Rare Tinea capitisTinea corporis

Trichophyton mentagrophytes Rare, thin walled, smooth*Hypha: COILED

Grape-like Tinea pedis, ungium, cruris, barbae

Trichophyton Rubrum Absent Teardrop Tinea corporis, pedis, ungium, cruris

Epidermophyton floccosum

Numerous, smooth walled MACROconidia

Thick-walled, pointed end MACROconidia

Microsporum canis

With spiny tips

Microsporum gypseum

Blunt end MACROconidia

Trichophyton mentagrophytes

Grape-like clusters (“en grappe”) microconidia

macroconidia

*Coiled hyphae

Trichophyton rubrum

Tear drop (enthyrse) microcondia

systemic mycosisHistoplasmosis

Histoplasma capsulatum

tuberculate MACROconidia

FUNGIFACTS

HistoplasmosisLaboratory Diagnosis: •KOH: organisms found inside histiocytes•Wright/Giemsa•Culture: SDA, BAP (Smith & Goodman)•Serology

•Skin test: Histoplasmin Ag•CF

opportunistic mycosesA. Aspergillosis

B. Candidiasis

C. Cryptpococcosis

D.Mucormycosis

E. Penicilliosis

Aspergillus fumigatus

conidiophore

conidia

vesicle

Biseriate phialide

- phialide

- metulaFUNGIFACTS

Aspergillosis

(+) Fungus ball- spherical mass of intertwined septate branching hyphal elementsLaboratory Diagnosis: •septate hyphae that branch at regular intervals seen in clinical specimens

Candida albicans

FUNGIFACTS

*Chronic Mucocutaneous Candidiadis

Laboratory Diagnosis:•(+)germ tube test (rapid diagnosis)

•(+)Budding yeast cells•(+)pseudohyphae •(+)septate hyphae

Budding yeasts

Germ tubes

Cryptococcus neoformans

Encapsulated yeast

FUNGIFACTS

CryptococcosisLaboratory Diagnosis: •India ink: encapsulated yeast

Mucor sp.

Sporangium*sporangiospores inside

FUNGIFACTS

Mucormycosis

Laboratory Diagnosis:•(+)coenocytic hyphae•(+) sporangium containing sporangiospores•*Ubiquitous in the environment and encountered as contaminants

sporangiospores

Coenocytic (aseptate) hyphae

Rhizopus sp.

sporangiospores

sporangium

rhizoids FUNGIFACTS

Mucormycosis

Laboratory Diagnosis:•(+) RHIZOIDS•(+) sporangium containing sporangiospores

Penicillium sp.

Chains of conidia

Branching conidiophore

Phialides

Subcutaneous mycoses

Sporotrichosis

Chromomycosis

Sporothrix schenckii

Daisy-like microconidia

FUNGIFACTS

Sporotrichosis (Lymphocutaneous)

•Dimorphic

•Yeast: cigar body (budding yeast cells)

•Mold: numerous micro conidia in a rosette/daisy pattern

Cladosporium carrionii

Long chain of conidia

FUNGIFACTS

Chromomycosis

•Dematiaceous

•Mold: branching conidiophore

•Chains of conidia

•Septate hypha

Phialophora verrucosa

Flask-shaped conidipohore

FUNGIFACTS

Chromomycosis

Verrucous dermatitis

•Dematiaceous

•Septate hypha w/ short conidiophore & collarate mass of conidia

Fonseca pedrosoi

Club-shaped conidiophore

Pattern of sporulation: acrotheca

Conidia @ lateral sides