1. MARILYN M. BALAIS LEIRA JIM V. TANGIAN MYRTLE ANNE FAYE N.
VIRATA ENTOMOPATHOGENIC NEMATODES AGAINST Ostrinia furnacalis
(ASIAN CORN BORER)
2. INTRODUCTION 3. Background of the Study Insects play
important parts in the ecosystem Entomopathogenic nematodes (EPNs)
as biopesticide to control the Corn borers Achroia grisella (Lesser
Wax Moth) as alternative EPN bait 4. About A. Grisella (lesser wax
moth) Wax moth is a serious pest in beehives and can cause
substantial losses of combs, damage to beehive material and spoil
beehive products. 10 to 13 mm long 5. Larval stage 6. GROUP OF
NEMATODES!!! THE GOOD THE BAD THE UGLY Entomopathogenic nematodes
(EPN 7. Entomopathogenic nematodes 8. Entomopathogenic nematodes 9.
Ecological indicators/Pollution studies Species Interaction studies
Biological Control Agents Genetics studies Molecular studies 10.
Why BIOLOGICAL CONTROL Agents? NO TO EXTENSIVE SYNTHETHIC CHEMICAL
APPLICATION 11. Entomopathogenic Nematodes (EPNs) in the genera
Heterorhabditis and Steinernema are used as biological control
agent (BCA) for diverse insect pests. Family : Steinernematidae
with 45 known Steinernema sp. Family : Heterorhabditidae with 11
known Heterorhabditis sp. The enemy of my enemy is my friend THE
AMBUSHERS THE CRUISERS EPN pest 12. Objectives of the Study The
study aims to apply isolated EPNs from soil samples from PSHS CMC
grounds against the Asian Corn Borer (Ostrinia furnacalis).
Specifically, the study aims to: 1. extract the EPNs present from
the wax moth larva; 2. identify the EPNs up to its species level;
13. Objectives of the Study 3. apply the extracted EPNs to the
larva of the corn borer; 4. note the mortality rate and qualitative
morphology descriptions of the effects of the EPNs on the Asian
Corn Borer larvae; 14. Significance of the Study EPNs as
biopesticide and growth control of insects Cheap and easy way to
obtain EPNs Introduces a new and environmental friendly way to
eliminate plant pests 15. Life-cycle of EPNs in vivo and in vitro
Culturing of EPNs 16. In the PHILIPPINES? Recent studies are
undergone in MSU-IIT and now in PSHS-CMC 17. Corn borers are one of
the major pests in the corn production industry. Eliminating pests
from corn can make a great impact in the Philippine agriculture 18.
Scope and Limitations of the Study Usage of the lesser wax worms as
bait for the EPNs from the soil sample Pathogenicity test in the
laboratory is limited to the counting of the number of dead Asian
Corn Borer larvae, as well as on noting on the qualitative effects
of the EPNs on the external morphology of the 19. Scope and
Limitations of the Study Only one site is selected within the
PSHS-CMC campus Collected EPNs will be used against the larvae of
the corn borer in the laboratory 20. MATERIALS AND METHODS 21.
Materials and Methods Flow Chart Wax Worms Subculture Pathogenicity
Test on Corn Borers Nematode Subculture Collection of Soil Samples
Quartering Method Nematode Baiting Artificial Media Preparation
Analysis of Data 22. How to obtain Dauer Juveniles from the Soil?
23. Ten-points Plotting Ten points represented by circle with 1
meter distance in each point will be made in the sampling area 24.
Quartering Method 25. Nematode Baiting After soil collection from
different areas, collect subsamples and place in a small cup (100g
soil only) Put at least 5 larval stage of A. grisella inside the
cup, cover using the lid and put the sample under room temperature.
After 24 hours, examine the larvae in the soil, if morbid leave for
another 24 hrs and if found dead after 3-7 days depending on the
species, collect the cadaver Rinse the cadaver using either
distilled water Transfer the cadaver to white trap 26. White Trap
27. Subculture of EPNs in RINGER's solution Apply subcultured EPNs
against the Corn Borers 28. Application of the Isolated EPNs
against Corn Borer last stage of the corn borer larva was used and
small plastic cups with filter paper were each filled with one
larva (1 larva/concentration times 3 replicates) times 3 trials
nematodes were counted by putting small drops of the solution on
the Petri dish and then the droplets were examined under the
spectroscope add drop/s with 5, 10, 20, 30, 40, 50 EPNs) on each
borer filter paper in the cup is moistened 29. 24-well plate The
same procedure will be done for the lesser wax 30. Gathering of
Data checked after 12, 24, 36, 48 and 72 hours to see if the corn
borers were dead Death of larva is caused by successful invasion of
EPNs if the cadaver shows discoloration and presence of EPNs when
dissected Note on the mortality rate per 31. Analysis of Data Corn
Borer as bait The t-test computed the permutation t-test p-value
t-test had been acquired using (PAST) software Pathogenicity Test
Probit analysis Abbot formula 32. Results and Discussion Corn Borer
as bait cadavers of the corn borer larva cup were then collected
and recorded on the 3rd, 5th, and 7th day of the baiting procedure
Discoloration Heterorhabditis group no significant differences
between the permutation t-test values since the results were more
than 0.05 Pathogenicity test LC50 of the pathogenicity test,
derived by probit analysis 44% control mortality 33. Figure 1.
Photo of EPNs seen under the dissecting microscope (magnification
40X) (taken on September 15, 2012, MSU - IIT). 34. Results and
Discussion Pathogenicity test Abbots formula n = Insect population,
T = treated, Co = control Other observations 35. Conclusions and
Recommendations corn borer larva can be used as an alternative EPN
bait corn borers are effective bait for Heterorhadbitis group 40
concentration/larva is best effective since it has the highest
percent mortality from the LC50 as well as in the Abbot mortality
after 12 and 72 hours suggest that the set-up should be monitored
regularly and new insect larva may be applied with the nematodes
recommended that there will more specific and 36. Thank you for
listening! We are now ready for your questions.
