Don’t let your cells escape!chips™ Arena

Don’t let your cells escape!

Benefits and key features • Cell confinement areas (adhesive discs of 80, 140, 225, 500 and 1000 μm in diameter) corresponding to magnifications of 63x to 5x • No cell loss during time lapse experiments• Easy cell tracking • Easy acquisition automation on multiple areas thanks to the integrated cell localization grid • Designed to be compatible with 1 or 4 well CYTOOchambers

RPE1 cells were monitored in 10× time-lapse phase-contrast microscopy to measure cell division time with respect to time of starvation and observe daughter cell fates. Colored dots are used as visual marks to follow individual cell fates: (i) the blue cell’s daughters were starved in late G1 and divided again; (ii) the red cell’s daughters were starved in early G1 and didn’t divide again; (iii) the green cell’s daughters adopted asymmetric behavior: one divided again and not the other. From Pitaval et al. J Cell Biol. 2010, 191(2): 303-12.

HeLa cells confined on 500μm Arena discs imaged under 5x magnification

CYTOOchips Arena provide users with cell confinement adhesive areas of different sizes compatible with observations under a wide range of magnifications (5x to 63x). The chips are particularly useful for monitoring highly motile cells as well as for cell proliferation, cell lineage and cell fate studies. The chips can also be used for standardized cell colony growth. Micropatterns are available coated in fibronectin (fluorescent or not) or as ready-to-coat. Protocols are available to coat proteins such as collagen, Poly-L-Lysine, laminin or Matrigel®.

Applications • Time-lapse measurements• Observation of highly motile cells• Monitoring of cell fate • Precise cell proliferation studies • Standardized cell groups • Cell colony growth• Precise cell cycle measurements

Example: Monitoring cell fate upon serum starvation CYTOOchambers™ for Live Cell ImagingCYTOOchambers are easy-to-use devices designed for time-lapse experiments on CYTOOchips 20x20. Available in 1- or 4-well formats, CYTOOchambers have a standard foortprint and fit into all 35 mm microscope stage adaptors.The CYTOOchip Arena has been designed to be compatible with both 1 well and 4 well CYTOOchambers.

CYTOOchip layout

The discs of different diameters (from 500 down to 80 μm) are organized over the chip in 4 identical quadrants. Each quadrant is again divided into 4 zones and each disc diameter width is arrayed over 3x3 blocks. The blocks separating the 4 quadrants feature the discs of 1000 μm (one disc per block).

Fibronectin, Discs 500 μm, RPE1 cells, 10x

2.0 h 12.0 h 36.0 h 50.0 h

Arena discs imaged at 5x

80 μm 225 μm 500 μm 1000 μm

4 well CYTOOchamber

1 well CYTOOchamber

chips Arena

Product specifications

Ordering information

SubstrateChip sizePatterned areaMicropattern geometriesDisc diameter, μmCorresponding objective (one disc in the FoV)Pattern area, μm²Micropatterns per blockNumber of micropatterns per chip Pitch between patterns, μmGap between patterns, μmAdhesion proteinAdhesive separation bands between blocksCell localization grid Error-free identification of patterned side Chip identification letter Compatibility with CYTOOchambers 35 mm Packaging

Cat. No.10-020-10-06 CYTOOchips Arena FN Glass 20x20 mm; 170μm; Gridded; Disc patterns; Diam. 80, 140, 225, 500 and 1000 μm; Fibronectin; set of 610-020-13-06 CYTOOchips Arena FN650 Glass 20x20 mm; 170μm; Gridded; Disc patterns; Diam. 80, 140, 225, 500 and 1000 μm; Fibronectin labeled 650 nm; set of 610-020-00-06 CYTOOchips Arena A Glass 20x20 mm; 170μm; Gridded; Disc patterns; Diam. 80, 140, 225, 500 and 1000 μm; Activated (ready to coat); set of 630-010 CYTOOchamber 1 well Ext. diameter 35 mm; with lid; 1 well; Max. imaging area 16.5 x 16.5 mm2

30-012 CYTOOchamber 2 wells Ext. diameter 35 mm; with lid; 2 wells; Max. imaging area 16.5 x 7.5 mm2

30-011 CYTOOchamber 4 wells Ext. diameter 35 mm; with lid; 4 wells; Max. imaging area 7.5 x 7.5 mm2 per well30-020 Microscope adaptor plate With clips; accept up to 6 CYTOOchambers

Product Description

High quality low fluorescence glass (170 μm thick, 1.5)20 x 20 mm² (CYTOOchip 20x20 format)16.9 x 16.9 mmDiscs from 80 to 1000 μm of diameter80 140 225 500 100063x 40x 20x 10x 5x5,000 15,400 39,800 196,000 785,00036 25 16 4 11,296 900 576 144 25217 260 325 650 1,300137 120 100 150 300Fibronectin (FN), FN650* or Ready-to-coat (A)**NoYesYes Yes 1, 2 or 4 wells By 6; in a blister pack sealed in aluminium bag under inert gas

* Fluorescent micropatterns (FN labeled with fluorophore with exc. =650 μm): useful for easier pattern identification or autofocusing as well as for automated image analysis over multiple patterns. ** Activated CYTOOchips for adsorption of the protein of your choice (Collagen, Laminin, Poly-Lysine, Matrigel®, specific antibodies etc.). Protein may be fluorescently labeled. Contact us for recommended coating protocols and specific needs.

view

box.

fr

Adhesive micropattern products provided by CYTOO are covered by European and US Patents and patents pending as well as their foreign equivalents. These products may be covered by one or more Limited Use Label Licenses. The purchase of this product conveys to the buyer the non-transferable right to use the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer this product to a third party or otherwise use this product for Commercial Purposes. By the use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. For research use only. Not intended for any animal or human therapeutic or diagnostic use.

For inquiries please contact us at www.cytoo.com/contact

Find here a list of relevant publications: www.cytoo.com/publications

IP Rights

Further Reading

Example of 500 μm discs (for 10x objective)

Pitch

CYTOO S.A. 7, parvis Louis Néel BHT 52, BP50 38040 Grenoble cedex 9 - FRANCE Tel. +33(0) 438 88 47 05

CYTOO, Inc Harvard SquareOne Mifflin PlaceSuite 400Cambridge, MA 02138Tel. +1 617 674 7711

www.cytoo.com - info@cytoo.com © 2009 CYTOO S.A. CYTOO™, CYTOOchips™, CYTOOplates™, CYTOOchambers™ and Reference Cell™ are trademarks of CYTOO S.A.

Gap