BiODX - Antimicrobial Technology Presentation

An industrial biotech company situated in the CSIR Mining Innovation Centre,

with over 6 years already dedicated to R&D in conjunction with the Council for

Science and Industrial Research (CSIR) to develop sustainable biocides.

Based on the development of DECONT-X, a mild organic biocidal active ingredient,

BiODX products have gained popularity due to its non-corrosive properties and non-

toxicity surrounding microbial decontamination.

Industrial Formulations

Food and Beverage

Health

Agricultural

Copyright BiODX 2013 All Rights Reserved

Who We Are?


• BiODX’s research was based upon a principal which assumed that triggering

an action on sensory parameters of citrus would yield high dosages of

biological yield from the target gene.

• BiODX’s innovative processes use the natural enzyme reaction occurring from

the time of picking the fruit, where degradation of the antimicrobial component

starts as the fruit converts all resources (sugars, bioflavonoids, antimicrobials

etc.) in order to stay alive.

• Innovation is also seen in the formation of two active compounds that do not

exist in the raw fruit; these two compounds are formed by virtue of the digestion

process that converts citric acid and vitamins.

• Formulating preservative into the juice is a stoichiometric reaction. With

reference to the formation of vitamin C, a citrus oxidation-reduction system has

electron donating and electron accepting properties.

The Technology


Active Citrus Extract

GC (gas chromatography) analysis identified active peak in citrus extract.

Bioflavonoids which is a main component of citrus has previously been reported

as having a growth inhibitory effect on bacteria such as colibacillus,

Staphylococcus aureus or methicillin-resistant Staphylococcus aureus.


GC (gas chromatography) analysis of DECONT-X showing activity compounds and

newly formed sub compounds post digestion.

Stabilised Citrus Extract

The reaction between the citrus extract and QAC is a stoichiometric reaction

causing an ion exchange between the two compounds allowing the big molecule

(citrus extract) to digest certain sugars and other elements into newly formed

compounds.


Regulatory Compliance

Originating in response to pollution laws in the USA, organic chemists use all the tools

and training of traditional chemistry to create non-hazardous chemicals and processes

that are "benign by design".

SABS SPECIFICATION

NUMBER

DESCRIPTION FDA & EPA (United States of

America) Regulation

SANS 636 Disinfectants based on Quaternary

Ammonium Compounds

400ppm

SANS 1615 Detergent-Disinfectants based on

Glutaraldehyde

400ppm

SANS 1828 Cleaning Chemicals for the Food

Industry

400ppm

SANS 1853 Disinfectants and Detergent

Disinfectants for use in the Food

Industry

400ppm

SABS MARK Quality Management System ISO Accredited

KOSHER Certification Eco-Friendly Disinfectant

HALAAL Pending


Product Comparative

Safety Matrix

Ingredient Toxicity Environmental

Concerns Handling

Sensory

Effect Corrosion Natural

Alcohols N N Y N N N

Aldehydes Y N N N N N

Paracetic Acid Y N Y Y Y N

Phenols Y Y N Y N N

Hypochlorite Y Y Y N Y N

Hydrogen

Peroxide N N Y N Y N

DECONT-X N Genopole N N N N

Organic chemistry describes a way of carrying out industrial activities from start (design)

to finish (production), it is driven by overwhelming consumer demands, regulatory and

economic factors.


BiODX Products VS.

Conventional Chemicals

Chlorine,

Heavy Metals,

Solvents,

Paracetic Acid,

Caustic Soda,

Aldehydes

Conventional 60% Natural 80% Natural

Pollution, effluent fines, corrosive,

toxic waste, kills enzymes,

damage to the environment

Earth Friendly, biodegradable,

non-corrosive, non-toxic,

harmless to the environment

Bring innovation to your customers to have differentiation from competition.


About the Product

• All South African

• Earth friendly and fully biodegradable.

• Bactericidal, fungicidal and virucidal.

• Pleasant characteristic fragrance due to citrus content.

• Has characteristic detergent properties.

• Unaffected by ammonia & nitrates.

• Not reactive with organic matter (no THM).

• Fast kill kinetics on both sessile and planktonic bacteria.

Properties of DECONT-X:


• Non-irritant at normal use levels

• Non-carcinogenic

• Non-mutagenic

• Non-embryotoxic

• Non-teratogenic

• Contains no alcohol, formaldehydes or other harmful chemicals.

• Chloride Free - does not generate any induced chemical corrosion regardless

the residence or contact time.

Safety Characteristics

SS


Compatibility,

Solubility & Stability

• DECONT-X is miscible with water and polar organic solvents.

• Stable in acid and alkaline pH environments from pH 2 up to pH 11.

• Compatible with a wide range of additives and auxiliary products including

surfactants, flocculants, dispersants, corrosion inhibitors and other biocides.

• In common with other non-oxidising biocides, strong reducing and oxidising

agents should be avoided.

• A potent antimicrobial with broad spectrum activity against Gram negative and

Gram positive bacteria, SRB’s, APB’s, anaerobes, yeasts and fungi.

• Possible anion replacement for stabilization under acid condition.


The “intelligence” of DECONT-X kills 99.9 % of pathogenic micro-organisms & MIC’s (microbials influencing corrosion).

Product Performance

• Escherichia coli sp.

• Pseudomonas sp.

• Staphylococcus sp. + Streptococcus sp.

• Listeria sp.

• Effective against legionella at as low as 30 ppm.

• Black, green & brown moulds (Stachybotrys)

• SRB’s, ARB’s & Anaerobes (MIC’s)

• Remarkably effective against Pseudomonas, Stachybotrys and MIC’s.


Performance

on Hard Surfaces

Mic

rob

ial C

ou

nt

The following results show the microbial control obtained at the beginning,

during and completion of a 3 month trial.


DECONT-X Benchmarking VS. Conventional Chemicals

The number of internal failures related to MIC and chemical corrosion issues

degrease by 80% with the use of DECONT-X.

Am

ou

nt

of

Bacte

ria c

fu/m

L


Comparative MIC Agent

Corrosion Rates

DECONT-X allows long residence time without generating any induced chemical

corrosion and offers a technical alternate to reduce dependency on Glut and THPS.

MPY

Autoclave Corrosion Results on 1018 Steel


• Oil / Petroleum (fuel additive)

• Fracking

• Pulp / Paper Mill Industry

• Water Treatment / Cooling Towers

• Biofilm Control

• Disinfectant Formulation

• Preservation / Coatings / Paint

• Printing Chemicals / Ink

Markets for DECONT-X

BiODX have positioned DECONT-X selectively in these markets where a

sustainable biocide will have a competitive advantage due to end-user concerns

related to the environment, product performance and toxicity.


Toxicity &

Corrosion Matrix

Conventional chemicals as demonstrated pose harmful risks to the labour force

promoting absenteeism and corrosive properties leads to equipment damage

resulting in huge financial losses.

Conventional

Chemicals

Toxicity /

Corrosion

Max Paracetic Acid Phenols

Hypochlorite Hydrogen Peroxide

Aldehydes

Min

Natural

Sustainable

Chemicals

Natural

Health /

Financial

Losses


Product Advantages

One of many comparative examples of sustainable VS. conventional chemicals

as applicable to cooling towers and there affect on water chemistry.

DECONT-X CHLORINE

Unaffected by ammonia & nitrates Affected by ammonia and nitrates

Non-toxic, no health & safety concerns Major health & safety concerns

pH Neutral Affects water pH

Perceived as a protein/food - no resistance Bacterial strains can resist it

Non-corrosive Highly corrosive

Outstandingly effective against biofilm No efficacy against biofilm

No reaction to organic matter Produces trihalomethanes / carcinogens

Stand alone product Require extra chemicals

Stable at 5°c to 112°c Unstable

At 15-25 ppm (in water) is cheaper than chlorine Higher costs


Time in Hours

- Control

- DECONT-X

Mic

rob

ial L

oad

(cfu

/cm

2)

Total Bacteria Control in

Cooling Tower Water

The water sample was taken directly from a cooling tower that had undergone

standard treatment of a two ton shock treatment of chlorine added every two weeks.

The control did not get treated with any additional biocide during the 72 hours.


• Stronger chemical concentrations

• Increased chemical costs

• Increased water / rinse costs

• Increased electricity / heating costs

• Increased process costs

• Increased labour / absenteeism costs

Bacteria do not identify DECONT-X as a poison and therefore do not imitate

development of resistance.

Bacterial Resistance

Bacteria learn to acquire immunity to conventional chemicals, which means:


The Knock-On Effect - slide 1 of 2

Conventional

Chemicals

Sustainable

Products

Sustainable Chemicals VS. Conventional Chemicals

The Knock-on Effect

Water Electricity Effluent Corrosion Absenteeism

Pro

du

ct

Co

st

Savings to a companies bottom-line are accrued by using sustainable chemicals, this

is the responsibility of the CEO as it influences many knock-on effects.


The Hidden Costs to the

Knock-On Effect slide 2 of 2

Conventional chemicals require heat, rinsing, water chemistry and affect the pH,

this mixture of chemicals is harmful to the environment, human health and is

highly corrosive thus affecting the companies bottom-line!

Cost Conventional DECONT-X

Product Cost € 3.00 € 7.50

Electricity € 0.40 € 0.00

Water € 0.20 € 0.00

Effluent € 1.00 € 0.00

Stabilising Chemicals € 2.50 € 0.00

Corrosion € 0.50 € 0.00

Absenteeism € 2.00 € 0.00

Total € 9.60 € 7.50


Marketing green & overcoming green-washing.

Are you Green or

Green-washed?

Become an authority!


Drop the Sentiment!

Time for green sentiment to get out of the boardroom!

Tree huggers out!

Green business is about making profits greener – with Industrial Ecology

Bunnies go home!


An economy based on the intelligence of natural systems!

What are we really

selling?


Graham Poultney

Tel: 011 358-0052

Cell: 082 447 3467

[email protected]

www.biodx.co.za

Contact Us

Tel: 011 358-0052

[email protected]

www.biodx.co.za

CSIR – Mining Innovation Centre

Building 2

Cnr. Carlow & Rustenburg Rd

Melville, Johannesburg

South Africa

mailto:[email protected]

http://www.biodx.co.za/

mailto:[email protected]

http://www.biodx.co.za/