1. www.stemrx.inStemRx Bioscience Solutions Dr. Pradeep Mahajan Stem Cells Transplant Expert | Founder @ StemRx Bioscience Solutions, India

2. www.stemrx.inYou carry your own natural repairing kits in your own body 3. www.stemrx.inAging and death do seem nature has planned for us...The questions is Can we slow down this process? Develop clock of aging Time.. 3 4. www.stemrx.inThey fought for amrut ..to become immortal4 5. www.stemrx.in"the land of the immortals"OKINAWA 5 6. www.stemrx.inMankind is always in search of how to prolong life & above all, to make old age worth living & purposefulEveryone6wantstolivelongerandlookyounger 7. www.stemrx.inAging ?- How we age Breakdown in the cellular safety nets Functional decline of molecular, cellular activities to multiple organ level Decrease in the vitality of the body to adopt various diseases Decrease in the reproductivityDeath is the Final event 7 8. www.stemrx.inAging / Senesces Causes of AgingGeneti c | DNA Damag eFree radical metabolis mAGEs (Advanced Glycation End Products)Telomer sGene of Aging daf2Gene of longetivity Aging reflects over all body with different pace Aging does not occur in same way in every human being 8Lifestyle, genetic make up has influence on aging 9. www.stemrx.in Organ/ function BloodWBC and RBC count decreasesFace / skinWrinkles, facial hair, thinning cheek, double chinHairGraying, thinning, baldingBonesLoss of bone cells as they become porous and brittleMusclesLoss of muscular massFatFat content and distribution change, storage increasesJoints 9Aging SymptomsCartilage becomes cracks and frays 10. Therapy Cognitive Behavirol Therapy(W C Bushwell 2006)www.stemrx.inWhat Enhancing Self 10healing capacities Mobilization of dormant or sleeping stem cells Activate body immune system Decreasing Free radicals Link betweenHow Diet Veg & Fruits, Green Tea Workout, Meditation, Breathing Exercises Coping the stress Calorie Restrictions Antioxidants Growth hormone supplements 11. www.stemrx.inAverage lifespan with Aging diseases11 12. www.stemrx.inDeath Causes12 13. www.stemrx.inSo the question is can we stop agingAn Adult Human once a tiny cell, is a self assembling machine made of trillions of microscopic components 13 14. www.stemrx.inLets see what are the ways ??? Gene Therapy Narrow Bandwidth Cell Therapy Broad Bandwidth14 15. www.stemrx.inHow to Delay or Reverse The Aging? Antiaging Remove the damage or Inactive moleculesand cells Restore function to several molecules by repair & Replacement Modify the Genetic Program to prevent the aging process from continuing How are we going to do Above changes...??? 15 16. www.stemrx.in16 17. www.stemrx.inWho Can take Anti-Aging Treatment Absolutely healthy aging (King of Good Times) Patients with age related diseases (StrugglerKing) Aging patients with serious & Advanced diseases(Bankrupt King) 17 18. www.stemrx.in Aging can be reversed by set of cells at alaboratory level??? Do you believe ?18 19. www.stemrx.in19 20. www.stemrx.in20 21. www.stemrx.inStem cells proportion with agingHow the aging reflects in this graph?21growing cells > apoptic cells (Child) Growing cells = apoptic cells (Mature person) Growing Cells < apoptic cells (Aging Starts)Aging has been linked to age related inabilities of stem cells 22. www.stemrx.in22Stem Cells Therapy for AntiagingSource : Life Extension Magazine Ju 23. www.stemrx.inWhat makes given stem cells to reach to target/damaged site? Hypoxia SDF1 HGF Platelets MSC are magic cells as they release plethora ofnatural factors like chemokines and cytokines as well the microenvironment is equally responsible. 23 24. THE ADVANTAGES Of Stem Cell Anti-Aging Treatmentwww.stemrx.in Stem Cells from your own body are used No chemicals or drugs are needed Better breathing and sleeping pattern. Energy levels restored Age spots fade Complexion brightens and skin softens No more expensive supplements or creams required Hormones are re-balanced Sugar levels controlled Heightened sexual drive Increased metabolism and surprisingly an average 4-7 kg weight loss. Hair color darkening and volume increasing. There is also a better control of blood pressure and improved vision Emotional and cognitive improvement occurs, memory 24 improves, concentration ability increases and your ability to 25. Future Enhancements New Cutting Edge Technologieswww.stemrx.in1. VSEL 2. High Telomerase activity cells 3. Therapeutic application of cloned cells 25 26. www.stemrx.inVSEL VSEL : Very Small Embryonic/ Epiblast-like stem cells26Potentially represent the most regenerative adult stem cells having embryonic characterization. Proposed as a viable alternative to human embryonic stem cells. They home to site of injuries, upregulate angiogenesis, down 27. www.stemrx.inFirst reported in mouse bone marrow in 2006 by Kucia M et.al characterized by CXCR4(+)SSEA-1(+)Oct-4+ markers. Later identified in human blood and bone marrow as well. In 2011 Sovalat H et.al supported the evidence of presence of VSEL in human bone marrow. These relevant cells might be issued from very small embryonic-like stem cells deposited in the BM during ontogenesis and reside lifelong in the adult BM, and that they could be mobilized into peripheral blood by granulocyte colony-stimulating factor. very small embryonic-like stem cells constitute a "mobile" pool of primitive/pluripotent stem cells that could be released from the BM into the peripheral blood under the influence of various physiological or pathological stimuli like injuries, hypoxia, physical exercises etc. 27 28. www.stemrx.in1.Studies have shown that VSELs are deposited in adult tissues as backup population for TCSC2. An increase in chronic caloric uptake may prematurely deplete VSELs from adult tissues, thus contributing to aging. By contrast, caloric restriction has an opposing beneficial effect on the tissue-residing pool of VSELs and longevity. 28 29. www.stemrx.in29 30. www.stemrx.inIsolation of VSELs from blood by aphaeresisInjection of purified VSELs in bodyFollowed by GCS factor mobilization Characterizat ion and enrichmentMixed with collagen sponge scaffolds30 31. www.stemrx.in References 1. Kucia M, Reca R, Campbell FR, Zuba-Surma E, Majka M, Ratajczak J, Ratajczak MZ, (2006) A population of very small embryonic-like (VSEL) CXCR4(+)SSEA-1(+)Oct-4+ stem cells identified in adult bone marrow. Leukemia. May;20(5):857-69. 2. Ratajczak MZ, Zuba-Surma EK, Machalinski B, Ratajczak J, Kucia M. (2008), Very small embryonic-like (VSEL) stem cells: purification from adult organs, characterization, and biological significance.Stem Cell Rev.,4(2):8999. doi: 10.1007/s12015-008-9018-0. Epub 2008 May 6. 3. Heider A, Danova-Alt R, Egger D, Cross M, Alt R. (2013),Murine and human very small embryonic-like cells: a perspective. Cytometry A.;83(1):725. doi: 10.1002/cyto.a.22229. Epub 2012 Nov 16.314. Sovalat H, Scrofani M, Eidenschenk A, Pasquet S, Rimelen V, Hnon P (2011) Identification and isolation from either adult human bone marrow or G-CSFmobilized peripheral blood of CD34(+)/CD133(+)/CXCR4(+)/ Lin(-)CD45(-) cells, featuring morphological, molecular, and phenotypic characteristics of very small embryonic-like (VSEL) stem cells. Exp Hematol. Apr;39(4):495505. doi: 10.1016/j.exphem.2011.01.003. 32. www.stemrx.in5. Aaron M. Havens, Yusuke Shiozawa, Younghun Jung, Hongli Sun, Jincheng Wang, Samantha McGee, Anjali Mishra,L. Susan Taichman,Theodora Danciu,Yajuan Jiang,Gregory Yavanian,Elizabeth Leary,Paul H. Krebsbach,Denis Rodgerson,and Russell S. Taichman, Human Very Small Embryonic-Like Cells Generate Skeletal Structures, In Vivo; STEM CELLS AND DEVELOPMENT Volume 00, Number 00, 2012 Mary Ann Liebert, Inc. DOI: 10.1089/scd.2012.032732 33. www.stemrx.in33