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Tissue Culture
What is Tissue Culture ?
• Refers to technique of growing plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium
Media
• must be sterile• autoclave at 250 F at 15 psi
for 15 minutes• Murashige and Skoog media is commomly used
Explant• portion of plant removed and used for T.C.
Patterns of multiplication
• stage II - light 100-300 foot candles
• callus - shoots - roots• stage III - rooting - light intensity 1000-3000 foot candles
THANK YOU FOR YOUR ATTENTION