IN DEPTH EFFICACY CHARACTERIZATION CRO

SYNCROSOME

In Depth Efficacy Characterization CRO

IN DEPTH EFFICACY CHARACTERIZATION CRO

Animal models Manipulation Read-outs

- Emesis & Nausea- Safety evaluation- Efficacy Studies

- Irritable Bowel Syndrome

- Inflammatory Bowel Disease

- Ulcer

- Gastric Emptying

- Intestinal Transit

- All type of treatments- Small molecules- Immunization- Nutraceuticals

- All routes of administration - po, ip, iv, sc, im, it, in,ir

- Sample collection- Organs removal- Blood - Fecal pellet

- Tissue preparation- Dissection of organs

- Emesis, vomiting- Nausea

- Abdominal Contractions

- Inflammation- Histology, Histopathology- Biomarkers (MPO activity…°- Cytokines dosages- Intestinal motility

OVERVIEW OF SYNCROSOME’S GASTRO MODELS

IN DEPTH EFFICACY CHARACTERIZATION CRO

EMESIS AND NAUSEA

IN DEPTH EFFICACY CHARACTERIZATION CRO

EMESIS AND NAUSEA

SPECIES : Ferret & Piglet

TECHNIQUE : Behavioral assessment by trained observers

PARAMETERS : Number of episodes of retching and vomiting or emesisNausea

REF. COMPOUNDS : Emetic and anti-emetic agents

IN DEPTH EFFICACY CHARACTERIZATION CRO

EMESIS: LIST OF EMETOGENS IN TWO SPECIES

Ferret Piglet

Ipecac syrupAVP

Morphine PDE4 inhibitor

Copper sulfate PGE2

Apomorphine PGF2a

Cyclophosphamide Apomorphine

PDE4 inhibitor PAF

GalantamineStaphylococcalenterotoxin A

PGE2 Metformine

Isolflurane LVP

LPS

IN DEPTH EFFICACY CHARACTERIZATION CRO

IRRITABLE BOWEL SYNDROME

IN DEPTH EFFICACY CHARACTERIZATION CRO

SPECIES : Rodent, Guinea pig, Piglet

INDUCTION : Irritation, stress

TECHNIQUE : Manometric recording

PARAMETERS : Contractions

REF. COMPOUNDS: NK2 antagonist, 5-HT3 antagonist, 5-HT4 agonist

IRRITABLE BOWEL SYNDROME (IBS)

IN DEPTH EFFICACY CHARACTERIZATION CRO

INFLAMMATORY BOWEL DISEASE

IN DEPTH EFFICACY CHARACTERIZATION CRO

SPECIES : Mice

INDUCTION : DSSPARAMETERS : Disease Activity Index

InflammationMPO activity Cytokines dosages (TNFα, Il-1β, Il-2, IFNγ)

REF. COMPOUNDS : Sulfasalazine

INFLAMMATORY BOWEL DISEASE (IBD)

IN DEPTH EFFICACY CHARACTERIZATION CRO

GASTRIC EMPTYING AND INTESTINAL TRANSIT

IN DEPTH EFFICACY CHARACTERIZATION CRO

GASTRIC EMPTYING AND INTESTINAL TRANSIT

MODEL : Charcoal test and Red Phenol test

SPECIES : Rats

INDUCTION : Pharmacological

PARAMETERS : - Gut motility- Gastric emptying

REF. COMPOUNDS : Atropine

IN DEPTH EFFICACY CHARACTERIZATION CRO