Session 7: Development of a female Q-fly lure

biosecurity built on science

Development of a female Qfly lure

Andrew Finlay, Summerfruit Australia&

Paul CunninghamResearch Leader, AgriBio, DEDJTR, VIC.


Female Qfly LuresFeeding

Egg laying

Bactrocera tryoni


Phase 1: Proof of concept

Blends containing fruit ripening volatiles attract female Qfly

Proof of concept

11V

• analytical chemistry• blend formulation• electrophysiology• cage trials

A SYNTHETIC FRUIT ODOUR TOATTRACT MATED FEMALE FLIES


Phase 2: Formulations & Field studiesFinding the right mix


Phase 2: Formulations & Field studiesFinding the right mix FEMALE PHEROMONES

Day 12 mated

Day 3 virgin

Deterrent?

• Solvent extraction• Rectal gland extracts• Insect emissions


Phase 2: Formulation & Field studiesFinding the right mix YEASTS Cryptococcus flavescens

Aureobasidium pullulans

Rhodotorula sp (2)

Hannaella sp (3)

Pichia fermentans

Sydowia eucalypti

C. flavescens


Phase 2: Formulation & Field studiesFinding the right mix Deterrents

• Females avoid larval infested fruit• Acetoin emissions


Phase 2: New technologies

Applying calcium imaging of the antennal lobe to insect lure development

Volatile-evoked responsesin the Qfly antennal lobe

Qfly brain

Improved methods for attractant formulation across insect pests

• blend synergy• volatile detection• valence


Fieldwork 2016/17Field trials:

2. Mated vs unmated capture rates• feeding vs egg laying lures

1. Trial lure formulations • x 4 formulations• New dispensers

Tatura (VIC)

Stanthorpe (QLD)


Benefits of female lure research

Commercial fruit growers / consultants Monitoring / surveillance Reducing Qfly damage Lowering within / between season populations Reducing urban Qfly populations Improved market access Reduction in production losses

Sterile Insect Technique Assessment of success Lowering populations

Scientists Olfactory research Developing new technologies for pest management


Thank you

Project TeamJane Royer (QDAF)Pawel Sadowski (QUT/CARF)Mikael Carlsson (Stockholm University, AL imaging)Tony Clarke (QUT)

Tommaso Villa (female pheromones)Owen Webb (acetoin emissions)Alex Piper (yeast analysis)

Summerfruit AustraliaAndrew Finlay (Chairman)Rowan Berecry

Science

Session 7: Development of a female Q-fly lure