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Dr Chalmers is a cell biologist based at the University of Bath, UK and he currently leads a team investigating the role of stem cell regulators in prostate cancer. In 2014 he was promoted to a Senior Lectureship. Dr Chalmers have published over 25 peer reviewed papers, been invited to national and international conferences and been awarded major research grants from research councils and charities. He is also the founder of CiteAb, a citation based antibody search engine. The idea of CiteAb was birthed through frustration with using failed commercial antibodies. CiteAb aims to provide a better way for researchers to find antibodies which are suitable for their experiments. CiteAb was spun out of the University of Bath in January 2014 and Dr Chalmers is the currently the Chief Scientific Officer. For more details about 1st international antibody validation forum please check on http://www.stjohnslabs.com/ac_cms/blog
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Andrew Chalmers @Chalmers_AD
CiteAb and Antibody Validation
1st International Antibody Validation Forum October 2014
Approaches to antibody validation
• There are multiple approaches to antibody validation.
• Ranging from simple controls to high throughput approaches.
b-Tubulin
72kD-
52kD-
42kD-
34kD-RASSF7
control RASSF7 KD
b-Tubulin
Recino et al Biochem J 2010
Application specific antibodies
• Antibodies may work in one application but not another.
Anti-GFP antibodies which are suitable for IF, FACS and ChIP but
not Western Blotting
Sanchez et al Monoclonal Antib. Immunodiagn. Immunother. (2014)
Species cross reactivity ?
?
Antibody validation is an ongoing process
• Some suppliers do more validation than others.
• No supplier can test an antibody for every application with every tissue from every species!
• When choosing an antibody it is important to look for independent validation.
Or a never ending struggle!
Types of third party validation
• Contract research organisations
• Antibody reviews
• Peer reviewed scientific publications
CiteAb: The Antibody Search Engine
• Ranked by citations
• Independent and free to list
CiteAb: Antibody search
CiteAb: An antibody page
CiteAb: A publication page
CiteAb: How do we keep going?
• Enhanced profiles
• Custom antibody services listing
• Providing citations
• Antibody market data
Company X’s share in the USA
Institution Share of
citations
University College London 17.66%
University of Cambridge 11.90%
University of Oxford 10.15%
University of Edinburgh 7.32%
King's College London 7.32%
Top Institutions for using neuroscience antibodies
Swiss-Prot Number Number of
antibodies
Protein W 0
Protein X 0
Protein Y 1000
Protein Z 100
How many antibodies are there to each human protein
It seems useful!
Thousands of returning users
Users from 160 countries
A high proportion link to antibody companies
UniversitiesBathUniversity of LondonCambridgeYaleHarvard
Research InstitutesNIH MITKorea advanced institute of science and technologyIndian institute of science education and researchInstitute of Cancer research.Karolinska Institute
Pharmaceutical companiesBristol-Myers SquibbGSKAstraZenecaPfizerNovartisEli Lilly
Once you have chosen your antibody
• You still need to validate it in your own lab!
• Cite the antibody properly when you publish your data
Acknowledgements
• CiteAb (@citeab)
Matt Helsby, Dave Kelly, Adam Pope, Katrina Kelly, Paul Leader and Mark Farmer.
• University of Bath (@uniofbath)
Biology and Biochemistry, RDSO (especially Graham Fisher) and the Legal Services Team.
