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1
MicrobeSoil
Prof. Liz WellingtonProf. David Scanlan
Dr Gary Bending Dr Alex Jones
Dr Jonathan MooreDr Ian Lidbury
Andrew MurphyChristopher Hale
Prof. Mark TibbettDr Tandra Fraser
Plant
Dr John HammondDr Andrew Goodall
PHOSPHORUS CYCLING IN THE SOIL-MICROBE-PLANT CONTINUUM
2
Fertiliser P
Soil P
Organic/microbial P
Non labile Soil P Labile P
Investigating phosphate cycling in agricultural soils
Plant driven processes
Microbial driven processes
How do these processes interact?
Which microbes are driving this process?
How do these processes change during the season?
Can we reduce inputs, by improving below ground processes?
Can we manipulate the root to optimise the cycling of phosphorus?
• Plants response to P depletion?
• Is there a difference between expressed genes and actively translated genes?
• Are genes “primed” for expression in response to stress?
0%
5%
10%
15%
20%
25%
Abun
danc
e (%
NSA
F)
0%5%
10%15%20%25%30%35%40%45%50%
PHOSPHORUS CYCLING IN THE SOIL-MICROBE-PLANT CONTINUUM
Table 1. Concentrations (mg/kg) of phosphorus forms and their percentages (in parentheses) of NaOH-EDTA extractable total P as detected by 31P NMR.
Sample Ortho-P Monoesters Diesters Pyrophosphate Total P
A 287 (80) 71 (20) 0 0 358B 262 (77) 77 (23) 0 0 339C 249 (70) 70 (22) 0 0 317
Tandra Fraser (Mark Tibbett), Phosphorus Cycling, University of Reading
Phosphorus biochemistry in the rhizosphere
• NaOH-EDTA extracts• 31P NMR analysis• P- soil