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A product launch power point for NGS sequencing qPCR Quantification kit. A unique kit directly measures library concentration, providing the fast and reliable solution for cluster density estimation for NGS.
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Molecular Cloning Laboratories
MCNextTM SYBR® Fast qPCR Library Quantification Kit- The Revolution is Here
Molecular Cloning Laboratories
Molecular Cloning Laboratories
The Only Ngs Library Quant Kit Using A Library Standard
Simpler workflow – direct (MC kit) vs. indirect (KAPA kit) cluster density conversionBroader dynamic range of measurement – 7-log (MC kit) vs. 6-log (KAPA kit) Superior accuracy – Phi X library (MC kit) vs. single DNA fragment (KAPA kit) as standard Reliable – more consistent in quantification comparing with KAPA kit
Molecular Cloning Laboratories
Kit Components
Component name Volume
2x MCNext SYBR Fast qPCR Master Mix 5 mL
10x Primer Mix 1 mL
Phi X Library Standards 10 µL
Storage Buffer 3 mL
50x Low ROX (optional) 200 µL
50x High ROX (optional) 200 µL
Molecular Cloning Laboratories
Kit Types
Kit type Description Cat #
Universal kit No ROX added to 2x qPCR Master Mix IQPQ-UN
Low ROX kitLow concentration ROX in 2x qPCR Master Mix
IQPQ-LR
Regular ROX kitRegular concentration ROX in 2x qPCR Master Mix
IQPQ
Molecular Cloning Laboratories
Broad Compatibility Coverage
Reference Dye Compatible Instruments
No ROXBioRad iCycler MiniOpticon, Opticon 2, iQ5; Roche LightCycler 480; MJ Research DNA Engine Opticon 2, Chromo 4; Corbett Rotogene 3000, 6000
Low ROX ABI 7500 qPCR Systems, ViiA 7, QuantStudio 12K Flex, Agilent Mx3000P and Mx4000
Regular ROX ABI PRISM 7000, 7700, 7900HT, ABI 7300 qPCR Systems, Ge-neAmp 5700, StepOne, and the StepOnePlus
Molecular Cloning Laboratories
Higher Signal Strength and Better Efficiency for Library Quantification
Higher fluorescence signal
Better efficiency
1.000
1.000 E+1
Cycle
10 15 20 25 30 35
MCNext Library qPCR Quantification Kit
KAPA SYBR FAST qPCR Kit®
Molecular Cloning Laboratories
Broader Dynamic Range for Library Quantification
10x better sample quantification range
Better efficiency
Broader dynamic range
MCNext Library qPCR Quantification Kit
KAPA SYBR
FAST qPCR Kit
0
5
10
15
20
25
30
-4 -3 -2 -1 0 1 2
Cty = -3.426x + 13.67R² = 0.9997
y = -3.423x + 14.078R² = 0.9998
®
Molecular Cloning Laboratories
Superior Accuracy for Library Quantification
Electropherogram from Agilent 2100 Bioanalyzer indicates library average size of 500bp size
The only kit on the world wide market using an NGS library to quantify NGS libraries
KAPA kit uses a 525 bp single cDNA fragment to quantify NGS libraries
Molecular Cloning Laboratories
High Quality of the Library Quantification Standards
Melting curve of library standards after PCR amplification
Single peak
No nonspecific amplification products
6.900 E-2
5.900 E-2
4.900 E-2
3.900 E-2
2.900 E-2
1.900 E-2
9.000 E-3
60.0 65.0 70.0 75.0 80.0 85.0 90.0
Der
ivat
ive
Temperature (˚C)
Molecular Cloning Laboratories
Better Quantification Consistency than KAPA kit
* Actual Illumina Phi X library concentration = 10 nM
