PowerPoint Presentation
Luciferase Applications in Environmental BiotechnologyBy: Sajad
Rafatiyan1
ContentWhat is bioluminescence LuciferinLuciferaseApplication
for marine animalsApplicationsMedicine Molecular
BiologyEnvironmentReferences2
What is bioluminescence
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luciferin
Bacterial LuciferinDinoflagellate LuciferinFungal
luciferinsVargulinFirefly Luciferin Coelenterazine4
Phylogenic tree of luciferase
1991 Hastinger and moorinEstimated it has at least 30 different
evolutionary origins5
luciferase
E.C. 1.13.12.7pH: 7-8.5T: 20-371962 Discovered by Shimo mura
from Aquorea aquorea (Starfish)Photinus pyralis (Firefly)M.W.:
61kDaFirst cloning of firefly luciferase: 1985Stractur detection:
1996 2 DomainN-terminal (1-435)C-terminal (441-550)Loop
(436-440)6
Luciferase activityAdenylation of D-LuciferinOxygenation of
LuciferiladnylatLuciferaseMg 2+
ATP + Luciferin + O2 Oxyluciferin + AMP + Ppi + CO2 + LIGHT7
Milky sea
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Application for marine animalsFinding or attracting
preyDefinition against predatorscommunication
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ApplicationsMedicine Molecular BiologyEnvironmentToys
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Application in medicineTissue imaging to diagnosing abnormal
metabolic activity
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Bioluminescence Imaging (BLI)
InfectionTumors growthMetastasisToxicologyInteraction between
Protein-Protein (BRET)
Detected by Charged-Cooled Device camera (CCD)
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Cell imaging
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Application in Molecular biologyPyrosequencingGene
reporter15
Pyrosequencing
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Gene reporter
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Application in environmentBiosensorsHeavy metalsToxic
compoundsToluene compoundsLandmine detectionProduce Light
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Heavy metalsCo-transcription of resistance genes with luciferase
gene
Serratia marcescensmer OperonHg resistance
Staphylococcus aerousCad OperonCd resistance
Cloning of this resistant genes in expiration vectors with Lux
gene in E.coli 19
Toxic compounds
Photobacterium phosphoreum
1999 Hollis Clone this genes in S.cervisciae20
Toluene compoundsPseudomonad putidapWWO plasmidCloning of fluc
reporter gene in tol OperonBiosensor for TNT
1998 WillardsonpGLTUR in EcoliUse fluc
2003 BrahamepGLTUR in P.ptiadaUse GFPMore effective
biosensorToluene compoundsActivation of XyIRActive transcription of
Pu genesResistance enzymes21
Landmine detection
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Genetic engineered plants
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Biotechnology for landmine detection
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Produce light
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Produce light
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Produce light
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Toys or fantasy things
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ReferencesFutra, D., 2014. Microencapsulated Aliivibrio fischeri
in Alginate Microspheres for Monitoring Heavy Metal Toxicity in
Environmental Waters. sensors, Volume 14, pp. 23248-23268.
Habib, M. K., 2007. Controlled biological and biomimetic systems
for landmine detection. Biosensors and Bioelectronics, Volume 23,
pp. 1-18.
Miller, S. D., 2005. Detection of a bioluminescent milky sea
from space.
Sharp, N. J., 2016. Rapid Detection of Viable Bacillus anthracis
Spores in Environmental Samples by Using Engineered Reporter
Phages. Applied and environmental microbiology, 82(8), pp.
2380-2387.
Steinberg, S. M., 1995. REVIEW OF ENVIRONMENTAL APPLICATIONS OF
BIOLUMINES CENCE MEASUBEMENTS. Chcmmphcre, 30(11), pp.
2155-2197.
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Thank you31
