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Image Analysis of Daphnia Ahyun Son

Image analysis of daphnia

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Image Analysis of Daphnia

Ahyun Son

Daphnia is widely used in biotoxicity study The change of heart rate after in-taking

toxic substance is the measure of biotoxic-ity

Unfortunately, counting heat beat with naked eye is not easy because it jumps around continuously.

Fixing Daphnia on slide glass is also not a good solution because it causes big stress to the animal

Background

Non-invasive measurement. Fully automated Big data, presented as time series

curve on 2-dimensional area, is gener-ated that can be further analyzed

Complex pattern can be visualized

Advantages of image analysis of Daphnia motion

Experimental setup

Thin-layer cell made with cover glass(10mm x 10mm x 1.2mm

Camera with close-up lensComputer for image analy-sis

Daphnia trapped in a air bubble

Image analysis

Original image

conversion Track center of gravity

1. Download ‘Roborealm’ software2. Make a script (see right)3. Run the script, COG and time is saved au-tomatically

Made of cover glass Daphnia lives for a long time Thickness can be adjusted Two cover glasses are sandwiched with

molten paraffin Cheap and easy

Mini glass aquarium

Grow Daphnia in a aquarium Feed Chlorella Make mini aquarium with cover glass Inject a Daphnia into it. Seal the opening with paraffin Put it on camera and run RoboRealm Run the script and collect data Feed different substance to Daphnia Make a software that display the COG, time data as a

2D curve

Method

Result

Result 1

A spike caused by error image analysis software

A Daphnia is moving along the edge.

After 48 hours, mother Daphnia is died. More than ten baby daphnia are hatched and gathered at this area

A Daphnia is left in the cell for 24 hours

Before Adrenaline injection

AfterAdrenalineinjection

BeforeAcetyl choline injection

AfterAcetyl choline injection

Beforeenergy drink injection

Afterenergy drink injection

Image analysis may provide more useful in-formation of biotoxicity

Motion pattern could be analyzed if neces-sary

Conclusion

1. RoboRealm : http://www.roborealm.com/tutorials.php

2. Image processing software – provided by Biolocs Co. Ltd. Korea

Reference