Glutamine methylation in Histone H2A is an RNA Polymerase I dedicated modification

Peter Tessarz1, Helena Santos-Rosa1, Sam C. Robson1, Kathrine B. Sylvestersen2,Christopher J Nelson1,3, Michael L. Nielsen2, and Tony Kouzarides1,*

Speaker : Indra Lasmana Tarigan (英德拉 )Advisor : Prof. Chien-Chia Wang (王健家教授 )

Seminar of Department Life Sciences, National Central University

Introduction

Modification Histone

Scheme Epigenetic Mechanism

Methylation in Lysine

(Heinrich, et.al, 2014)

Result

Identification and localization of methylated H2A glutamine 105

Identification of Nop1/Fibrillarian as the methyltransferase of Q105

b

C

Genomic landscape of Q105me

Genomic landscape of Q105me

Unmodified Q105 is part of a recognition motif for FACT

Unmodified Q105 is part of a recognition motif for FACT

Discussion

Thank you for your attention

Replication through Nucleosomes

Immunofluorescence Microscopy

Coomassie Brilliant Blue

CFU