N a t i o n a l I n s t i t u t e Fo r C e l l u l a r B i o t e c h n o l o g y

Increasing Biopharma production

efficiency using DECOY-7N a t i o n a l I n s t i t u t e f o r C e l l u l a r B i o t e c h n o l o g y ,

D u b l i n C i t y U n i v e r s i t y , D u b l i n , I r e l a n d

e m m a . o n e i l l @ i n v e n t . d c u . i e

Biopharma Challenges• High Cost of Biologics Production

• Greater Demands on Cell Line Development• Higher Titres• Higher Cell Densities

• Greater pressure on pipelines (turnaround, etc.)

Greater Efficiency

Improving processes using miRNA s

microRNAs:• Post-transcriptional regulators• Many targets• Known to influence many processes

in cell• Not translated

miRNA-7 as a Process Target• microRNA-7 (miR-7)• Active following temperature shift (37°C-31°C)• miR-7-depletion tool: DECOY-7 • Functional studies demonstrate miR-7 depletion has a beneficial

effect on CHO bioreactor performance:• increased cell density • increased viability • US8,476,244) EP 2046970 B1

Gammell et al. 2007. Initial identification of low temperature and culture stage induction of miRNA expression in suspension CHO-K1 cells. Journal of

Biotechnology, 130, 3, pp213-218

Barron et al. 2011. Engineering CHO cell growth and recombinant protein productivity by overexpression of miR-7. J.Biotechnology 151(2):204-11

Sanchez et al. 2013. MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple genes including Skp2 and Psme3. PLOS One. 8(6):e65671

increased product yield

DECOY-7: Cell Density

Ctrl K1

DECOY-7 K1

DECOY-7: Viability

Ctrl K1

DECOY-7 K1

DECOY-7: Cell Productivity

Ctrl K1

DECOY-7 K1

What DECOY-7 means for you

• These features together have the potential to:• increase per-run profitability,• decrease time required to deliver requisite titres,• facilitate downstream purification

How we can work with youCollaborate to find a comprehensive solution to your upstream cell line development challenges;

- Transient transfection of your existing processes (as a media additive)

- Licence/technology transfer to implement DECOY-7 under your in-house control

- Collaborative research on your specific cell lines at the NICB

Scientific TeamBiopharma & CHO Cell Engineering Group

Multidisciplinary research centre, with over 25 years experience in integrated research in fundamental and applied cellular

biotechnology, molecular cell biology and biological chemistry

The mission of the NICB is to provide targeted and applied solutions to challenges facing the Biopharma industry, utilising our unique

multi-disciplinary team, our extensive clinical & industry network and our collaborative problem-solving expertise to deliver value for

stakeholders, collaborators and patients.

N a t i o n a l I n s t i t u t e Fo r C e l l u l a r B i o t e c h n o l o g y

Contact detai ls N i a l l B a r r o n , D i r e c t o r , N I C B

e : n i a l l . b a r r o n @ d c u . i e / t : + 3 5 3 1 7 0 0 5 8 0 4

Pa d r a i g D o o l a n , S e n i o r Re s e a r c h Fe l l o w , N I C B

e : p a d r a i g . d o o l a n @ d c u . i e / t : + 3 5 3 1 7 0 0 5 7 9 6

E m m a O ’ N e i l l , D i r e c t o r o f B u s i n e s s D e v e l o p m e n t , L i f e S c i e n c e s

D C U e : e m m a . o n e i l l @ i n v e n t . d c u . i e /

t : + 3 5 3 1 7 0 0 7 7 4 1