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Consider Safety: Preparing Samples for Cryopreservation
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- 1. CONSIDERSAFETY: PreparingSamplesforCryopreservation
- 2. LOWTEMPERATUREPROTECTION
Whenhandlingcryopreservedsamplesandcold temperatures,stashouldwear:
Paddedgauntletsforhand/armprotection Long-sleevedlabcoats
Closed-toefootwear Afull-facevisor
- 3. SAMPLEPREPARATION
Additivesareaddedtocellswhenpreparingthemfor
cryopreservationtoprotectthecellsfromdamage:
dimethylsulfoxide(DMSO) polyethyleneglycol glycerol
DMSOiseasilyabsorbedthroughskin.Long-sleevedlabcoats,
faceprotectionandglovesshouldbewornduringpreparation.faceprotectionandglovesshouldbewornduringpreparation.
- 4. BIOHAZARDSANDCRYOPRESERVEDSAMPLES
Whendealingwithbiosamples,stashouldtakethefollowing precautions:
Beawareofthebiosafetylevels Properlylabelsamples
Practicetheappropriatesafetyproceduresforthegiven samples
- 5.
- 6. CONTAMINATION
Pathogenscansurvivethelowtemperatures.Itisimportant
towipetheoutsideofvialswithgauzesoakedinalcohol beforeopening.
Wherecontaminationissuspected,stashouldfollow
appropriatedecontaminationproceduresforcleaning
liquidnitrogenstoragetanks.
- 7. SOURCES
ConsiderSafety:PreparingSamplesforCryopreservationBlog
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