ISHS Acta Horticulturae 628: XXVI International Horticultural Congress: Issues and Advances in Postharvest Horticulture

ANTIFUNGAL ACTIVITY AGAINST POSTHARVEST FUNGI BY EXTRACTS AND COMPOUNDS OF PITHECELLOBIUM DULCE SEEDS (HUAMUCHIL)Authors:   L. Barrera-Necha, S. Bautista-Ba?os, L. Bravo-Luna, K. Bermudez-

Torres, M. Jimenez-Estrada, R. Reyes-Chilpa, F.J. García-Suárez

Keywords: Triglycerides, triterpene saponins, Pitheduloside, Fusarium oxysporum, Penicillium, Rhizopus stolonifer, Colletotrichum gloeosporioides

Abstract: Natural products may offer a new approach for control of postharvest diseases of horticultural products. Pithecellobium dulce is an evergreen tree indigenous to Americas widely distributed in Mexico. We have previously reported significant antifungal activity of powder and aqueous extracts from the seed and leaves. In order to identify the active compounds present in the hexane-dicloromethane and methanol-water extracts from seed powder, a biodirected chromatography was performed. The fractions and compounds were tested against various postharvest fungi. The most sensitive strain was Fusarium oxysporum, ten fractions tested retarded its growth, while seven of the fractions retarded development of Rhizopus stolonifer. The less sensitive strain was Penicillium digitatum. The 1H NMR spectrum of two active fractions indicated the presence of two triacyl glycerols: Glycerol 1,3-dilinoleoyl-2-decanoic; Glycerol 1-linoleoyl-2-docosanoic-3-olein. Several triterpene saponins: Pitheduloside A, B, E, F and I isolated from the methanol-water extracts inhibited or retarded in vitro mycelial growth of Rhizopus stolonifer and Colletotrichum gloeosporioides, respectively. Further investigation should be undertaken to determine the effects of these compounds with applied studies on fruit.

ISHS Acta Horticulturae 628

XXVI International Horticultural Congress: Issues and Advances in Postharvest HorticultureList price  

€ 168     This title is available both in print and ActaHort CD-rom format

Conveners   E.W. Hewett, R.K. Prange 

Editor   R.K. Prange 

Publicationdate  

12 December 2003 

ISSN   0567-7572 

ISBN   9066054786 

Number of articles   111 

Volumes   2 

Place   Toronto, Canada