HEMOLYTIC ANEMIA CH.VYSHNAVIMSC MLT 1st YEAR
CLASSIFICATIONDEFINITIONHaemolytic anaemia is a condition in which red blood cells are destroyed and removed from the bloodstream before their normal lifespan is over.
Haemolytic anaemia are the anaemia's that result due to increase in the rate of red cell destruction.
About 1%blood is destructed ever day in normal adultRBCs normally survive 120 days . Bone marrow has the capacity to increase erythropoiesis 6 - 8 times than normal.Therefore Hemolytic Anemia may not be seen until the red cell lifespan is less than 30 days.Anemia is the result of premature destruction of red cells exceeding the erythropoietic capacity of the bone marrowWhen blood cells die, the body's bone marrow makes more blood cells to replace them. However, in haemolytic anaemia, the bone marrow can't make red blood cells fast enough to meet the body's needs.
An increase in destruction and production of erythrocytes can result in a compensated haemolytic state without anaemia being present so a called compensated haemolytic disease
Red cell destructionExtravascular IntravascularRESHaemGlobin Plasma iron poolPlasma protein poolProtoporphyrinExpired COUnconjugated bilirubinLiverConjugated bilirubinGI tractUrobilinogenFaecesUrineFree plasma HbHb- Hpt complexLiver Hpt and HpxHaemopexin-methem complx Excess HbKidneyHaemosiderinuriaHaem+globinHbmethemHemoglobinuria
6CLASSIFICTIONHemolytic anemias are classified in a variety of ways
Location of hemolysis INTRA VASCULAR (vascular space)
EXTRA VASCULAR(reticuloendothelial system)Source of defect causing hemolysis: Intra corpuscular defectExtra corpuscular defect
Mode of onset:Hereditary Acquired
INTRA CORPUSCULAR DEFECTSHereditary
AcquiredRed cell Membrane defect spherocytosis elliptocytosis
Red cell Enzyme deficiency G-6-PD deficiency PK deficiency
Deficient globin synthesis ThalassemiasStructurally abnormal globins [hemoglobinopathies] sickle cell anemia Unstable haemoglobins
Paroxysmal nocturnal hemoglobinuria
9EXTRA CORPUSCULAR DEFECTSImmune haemolytic anaemia(antibody-mediated destruction) Autoimmune haemolytic anaemiaWarm antibody auto immune haemolytic anaemiaCold antibody auto immune haemolytic anaemiaParoxysmal cold haemoglobinuria
Alloimmune haemolytic anaemiaHaemolytic disease of the newbornHaemolytic transfusion reactions : mismatched blood transfusion Microangiopathic haemolytic anaemiaDisseminated intra vascular coagulation(DIC)Hemolytic uremic syndromeThrombotic thrombocytopenic purpura
Sequestration(hyperspleenism & Spleenomegaly)
Drugs and chemicals
11Physical/chemical traumaThermal injury/burnsMarch haemoglobinuria
Infections and infectious agentsMalariaClostridium infectionsBartenellosisCholeraAnimal venoms
Symtoms of HemolysisPaleness of skinFatigueFeverConfusionLightheadednessDizzinessLab findings of Hemolytic Anemia Hemoglobinuria & hemoglbinemia
Peripheral smear- anisocytosis , poikilocytosis
Haptoglobin &hemopexin -decreased
Indirectbilirubin - increased.
Increased serum and stored iron.
Increased activity of bone marrow (erythroid hyperplasia)
Urine & feacal urobilinogen - elevated
Red cell Membrane defect
The most common defect of red cell membrane due to deficiency of cytoskeleton protein
- SPECTRIN - ANKYRIN - BAND 3
Deficient of membrane protein causes change of shape (round, no central pallor)
Hereditary spherocytosis is caused by a genetic defect. RBCs: abnormally small, round without center pallor.
Osmotic fragility is increased there is shift of curve to right
Hereditary elliptocytosisRed cell membrane defect.Abnormality in the skeltal protien
Causes : 1. Defective spectrin chain 2. Abnormality in the integral protiens 3. Deficiency or defect in band 4.1 4. Abnormally permeable to Na
Cells become elliptical due to the stress after entering the microcirculation.
Normal cells remains without any change.
21RED CELL ENZYME DEFICIENCIES
Glucose-6-Phosphate-Dehydrogenaze (G6PD) deficiency G-6-PD catalyses the conversion of g-6-phosphate into ribulose 5 phosphate
A deficiency of this enzyme reduces the ability of RBCs to with strand the effect of various oxidising drugs/agents, resulting in haemolytic anaemia
Fava beans High feversSevere infections Anti malarials-primaquine /quinine and choloroquine
In G6PD RBCs show moderate degree of anisopoikilocytosis with polychromatophilia, microspherocytes and bite cells
Pyruvate kinase deficiencyPyruvate kinase deficiency is an autosomal recessive disorder which causes congenital chronic hemolytic anemia In pyruvate kinase deficiency ATP is not formedATP is required for maintaining cell permeability.Potassium is lossed result in dehydration and hemolysis.The peripheral blood film shows normocytic normochromic anemia and increased reticulocytesP-smear shows : Irregularly contracted cells Echinocytes.
Sickle cell anaemiaSubstitution of valine in place of glutamic acid at the 6th position of chain Mutant Hb is the Hb-SHb-S : minimum solubility in deoxygenated state.Deoxy Hb is formed and polymerizeRBC become stiff and sickle shapeExtravascular hemolysis
30DIAGNOSTIC TESTSHb electrophoresis
HPLC: high performance liquid chromatography is useful for confirmation of diagnosisThalassemiasThe thalassemia syndrome is a heterogeneous group of inherited disorder in which one or more globin chain synthesis is either absent or reduced.
Thalassemia syndrome results in microcytic hypochromic anemia due to decreased production of hemoglobin with erythroid hyperplasia of the bone marrow.
The imbalance of globin synthesis causes an excess of the normally produced globin chain. This results in damage to red blood cells or their precursors and hemolysis
Thalassemia syndrome is mainly of two types,
1. Alpha thalassemia 2. Beta thalassemia
-THALASSEMIA -Thalassemia major(homozygous) -Thalassemia minor(trait) -Thalassemia intermedia -THALASSEMIA -Hydrops foetalis -Hb-H disease --thalassemia trait
THALASSEMIA ASOCIATED WITH OTHER HAEMOGLOBINOPATHIES -Hb-S thalassemia -Hb-E thalassemia -Hb-D thalassemia -HPFH(Hereditary Persistence of Foetal Hb)
ACQUIREDParoxysmal nocturnal hemoglobinuriaParoxysmal nocturnal hemoglobinuria is a rare , acquired , potentially life-threatening disease.Characterized by complement-induced intra vascular hemolytic anemia(anemia due to destruction of red blood cells in the blood stream),red urine(due to the appearance of hemoglobin in the urine)It is the only hemolytic anemia caused by an intrinsic defect in the cellIt may develop on its own or in the context of other bone marrow disorders such as aplastic anemia36
DiagnosisThe gold standard is flow cytometry for CD55 and CD59 on red blood cells. Based on the levels of these cell proteins, erythrocytes may be classified as type I, II, or III PNH cells. Type I cells have normal levels of CD55 and CD59; type II have reduced levels; and type III have absent levels.The fluorescein-labeled proaerolysin (FLAER) test is being used more frequently to diagnose PNH on WBCs (neutrophil and monocyte).
EXTRA CORPUSCULAR DEFECTSImmune haemolytic anaemiaImmune haemolytic anaemia is a group of anaemia's in which premature red cell destruction is mediated by antibodies that bind to red cells.
The antibodies may be either allo or auto antibodies
The haemolysis is caused by extracorpuscular mechanism
The site of haemolysis may be extravascular or intravascular
Classifications of immune hemolytic anemiasAlloimmune hemolytic anemia Hemolytic disease of new bornHemolytic transfusion reactionAutoimmune hemolytic anemiaWarm antibody type (IgG antibodies active at 37Oc)Primary(idiopathic)SecondaryAutoimmune disorders(systemic lupus erythematosus
ALLOIMMUNE HEMOLYTIC ANEMIAIn alloimmune hemolytic anemia allo-antibodies are present either in serum or bound to red cells
Hemolytic disease of the new born(HDN)
This is an immune hemolytic anemia which affects the fetus and newborn babyIt is due to maternal foetal blood group incompatibility.
HDN develops when mother lacks an antigen that expressed by the foetal red cells
HDN may be due to incompatibility of Rh type or ABO group.
AUTOIMMUNE HEMOLYTIC ANEMIAAutoimmune hemolytic anemia(AIHA)are a group of disorders in which antibodies against self-antigens on the RBC membrane cause premature destruction of RBCs.
Interaction of the autoantibody with the red cell antigen is dependent on the temperature, i.e. warm or cold type.
Warm antibody type
Warm antibody type is the most common type(50to 70%) of immunohemolytic anaemia
This can be further divided into idiopathic (primary)or secondary to drug exposure or predisposing diseases.
The antibodies are mainly of IgG type.
The antibodies combine with red cell antigen at 34c (warm antibody).
There IgG bound RBCs bind to Fc receptors on phagocytes (macrophages)