Embed Size (px)
Citation preview
Staining
ParaffinCelloidin
Collodionization
General Staining ProcedureXylene(1)
Xylene(1I)
DEPARAFFINIZATION
Absolute Alcohol (I)
Absolute Alcohol (II)
90% Alcohol
70% Alcohol
Water
SECTIONS TO ALCOHOL
STAIN
70% Alcohol
90% Alcohol
Absolute Alcohol (II)
Absolute Alcohol (I)
Xylene
Xylene
MOUNT
DEHYDRATION
CLEARING
Staining of Paraffin Sections
Staining of Paraffin SectionsXylene(1)
Xylene(1I)
DEPARAFFINIZATION
Absolute Alcohol (I)
Absolute Alcohol (II)
90% Alcohol
70% Alcohol
Water
SECTIONS TO ALCOHOL
STAIN
70% Alcohol
90% Alcohol
Absolute Alcohol (II)
Absolute Alcohol (I)
Xylene
Xylene
MOUNT
DEHYDRATION
CLEARING
Staining of Celloidin Sections
Staining of Celloidin SectionsCellulose NitrateSoluble in alcohol, treatment
avoidedPRECAUTIONS
◦Stains on skin◦Dirty or oily slide◦Faulty staining solution◦Too much adhesive
Collodionization of SectionsDefinitionIf paraffin ribbons
◦Contain air bubbles◦Torn◦Inadequately infiltrated
PurposeProcedure
