Staining

ParaffinCelloidin

Collodionization

General Staining ProcedureXylene(1)

Xylene(1I)

DEPARAFFINIZATION

Absolute Alcohol (I)

Absolute Alcohol (II)

90% Alcohol

70% Alcohol

Water

SECTIONS TO ALCOHOL

STAIN

70% Alcohol

90% Alcohol

Absolute Alcohol (II)

Absolute Alcohol (I)

Xylene

Xylene

MOUNT

DEHYDRATION

CLEARING

Staining of Paraffin Sections

Staining of Paraffin SectionsXylene(1)

Xylene(1I)

DEPARAFFINIZATION

Absolute Alcohol (I)

Absolute Alcohol (II)

90% Alcohol

70% Alcohol

Water

SECTIONS TO ALCOHOL

STAIN

70% Alcohol

90% Alcohol

Absolute Alcohol (II)

Absolute Alcohol (I)

Xylene

Xylene

MOUNT

DEHYDRATION

CLEARING

Staining of Celloidin Sections

Staining of Celloidin SectionsCellulose NitrateSoluble in alcohol, treatment

avoidedPRECAUTIONS

◦Stains on skin◦Dirty or oily slide◦Faulty staining solution◦Too much adhesive

Collodionization of SectionsDefinitionIf paraffin ribbons

◦Contain air bubbles◦Torn◦Inadequately infiltrated

PurposeProcedure