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Purifier® Logic® Class II Biological Safety Cabinet
The material included in this presentation is copyright of Labconco Corporation.
Reproduction or use of the slides is not permitted without express permission from
Labconco Corporation.
COPYRIGHT NOTICE
Fume Hood or Biological Safety Cabinet?
Fume HoodsFume Hoods -- Enclosures that capture, contain and remove chemical fumesand vapors.
Biological Safety CabinetsBiological Safety Cabinets --Enclosures that capture, contain and remove biohazardous aerosols.
Purifier® Logic® Class II Biological Safety Cabinet
Optional 254 nm UV lamp
Bright, glare-free fluorescent lighting
Fully-closing, counterbalanced tempered safety glass sash
Large 21.7” sash opening height
Leak-tight Type 304 stainless steel liner
Type 304 stainless steel dished work surface with lift out knobs
Contain-Air Negative Pressure Channel
Innovative LCD information center display
Easy-to-clean touchpad controls
Reserve-Air Secondary Airflow Slots
Curved stainless steel inlet grille
Accessory Base Stand (sold separately)
Being Green is being responsible
Labconco is a member of the United States Green Building Council (USGBC).Three Labconco product lines contribute to LEED® points:
Purifier® Logic® Biosafety CabinetsFlaskScrubber® and SteamScrubber® Laboratory Glassware WashersProtector® XStream® Laboratory Fume Hoods
Purifier® Logic® Biosafety Cabinet
Purifier® Logic® Motor
New motor technology• Electronically Commutated Motor (ECM)• Over 3 million in use
Customer Advantages• Quieter • Cooler Operation • Energy savings of 60%• More reliable for correct airflow maintenance• Continuously outputs information to be
displayed and triggers alarms when out of parameters
Intelligence Built In
Purifier® Logic® Biosafety Cabinet
Why is it cooler and quieter?• The ECM is more energy
efficient ~ less energy is wasted as heat
Purifier® Logic® Biosafety Cabinet
How do we know it is running okay?• The motor is programmed to maintain constant
airflow as the filter loads, no sensors needed
How does that work?• Each BSC is tested at various airflows and
volumes• Software uses data to generate a motor-blower
curve programmed into each motor• The motor remembers the speeds and torques
required as filter pressures change
Intelligence Built In
Purifier® Logic® Biosafety Cabinet
How do we know the ECM is running correctly?• The ECM continuously outputs information to be displayed and
triggers an airflow alert when the motor speed changes suddenly
How do we know to change the filters?• Motor initial and maximum speeds are calculated and filter life
remaining is figured
The conventional motor used in biosafety cabinets is a Permanent Split Capacitor (PSC) motor. It is known as an induction motor.
Labconco Delta (pre-2008)NuAire LabGardESCO Labculture
ECM PSC motorPermanent magnet on the rotor Magnetic field must be inducedMore efficient Generates a lot of waste heatPerformance feedback No feedback
Purifier® Logic® Biosafety Cabinet
How the Logic ECM is betterHeat is the #1 killer of motors because it reduces the life of the bearing/bushing lubricant. Because the PSC runs hotter, it will fail faster than an ECM.The ECM uses hardened ball bearings on the rotor-most PSC motors use simple bushings. Cooler operation and bearings in the ECM means a much longer lifespan for the ECM…rated to 50,000 hours.
Purifier® Logic® Biosafety Cabinet
Logic ECM energy comparison
The ECM saves significant energy compared to other motor technologies used in biosafety cabinets.
Power Consumption (Watts)*
ECM PSC 3-Phase4' BSC** 290 582 4806' BSC** 490 1440 804
*At 120V** 10" sash height
Purifier® Logic® Biosafety Cabinet
Purifier® Logic® Biosafety Cabinet
LCD Information CenterLine-of-sight on inside wall
Filter status
Cabinet status
Error messages
Icons indicate features on/off
Interval or “stop watch” timer
Fluorescent/UV Hour meter with alarm
LCD Information CenterStatus area
• “OK”• “Sash is too high”• “Airflow Alert”• “System Error”
Data area• Displays the filter remaining,
inflow and downflow velocitiesIcon Area
• Displays icons of active features
Purifier® Logic® Biosafety Cabinet
IconsNormal blower operation
Night-Smart blower operation
Fluorescent light
UV light
Timer
Security Lock
Mute
Purifier® Logic® Biosafety Cabinet
Purifier® Logic® Biosafety Cabinet
Simple interior design• Easy to clean• Electrical duplex up and
out of work surface area• Accessories keep work
area uncluttered• Improved lighting
Dual fluorescent lamps instead of one
Purifier® Logic® Biosafety Cabinet
Simple interior design• No thumb screws • Lifting knobs on both ends• Work surface support to assist for
removal• Towel catch/Pre-filter retained
without fasteners• Smooth work surface
Towel Catch
Work Surface Support
Purifier Logic® Biosafety Cabinet
Sash Changes• Viewing height increased 1.75"• Angled sash with no sash handle
across the bottom edge for better viewing
• Formed handles attached to sash for easy gripping
Purifier® Logic® Biosafety Cabinet
Lower exterior height • Approx. 3/4" lower overall
Greater interior height• Approx. 2" taller in the front
Increased viewing height
Dimensions
Purifier® Logic® Accessories
Improved Filter Options• Accept ULPA filters• Optional pre-filters• Optional carbon post-filters on 4' - 6' models
Carbon Filtered ExhaustCarbon Filtered ExhaustStickStick--on Foam on Foam PrefilterPrefilter
Purifier® Logic® Biosafety Cabinets
Telescoping Base Stand
Adjusts in 1 inch increments from 27" to 34.5"NSF-listed and ADA-compliantDurable epoxy coatingAvailable with fixed leveling feet or toe-locking polyurethane castersSeismic models available
Accessories
Purifier® Logic® Biosafety Cabinets
Hydraulic Lift Base Stand
Built-in electric or manual hydraulic lift
Height is adjustable from 25.5" to 33.5"
Supports loads up to 1000 pounds
Available in 3', 4', 5' and 6' widths
NSF-listed and ADA-compliant
Left: Manual Hydraulic Lift Base Stand is shown with Caster Kit installed.
Accessories
Purifier® Logic® Biosafety Cabinets
Optional Wire Shelves (stainless steel snap-in)Optional Cord/Tubing management hooks
Accessories
Purifier® Logic® Biosafety Cabinet
Stainless Steel TurntableVacuum Aspiration Pump
Accessories
Airflow Sensor Kit (not shown)
• Kit allows for installation of airflow sensors in the field• Optional sensor can be factory installed• Recognizes sensor and displays inflow (after calibration)
Purifier® Logic® Biosafety Cabinet
Accessories
IV Bar supports intravenous solution bottles and bags
UV Light Kits include one 254 nm UV lampService Fixture
Purifier® Logic® Biosafety Cabinet
Provides convenient supply storage
Fits easily under the Purifier Logic Class II Safety Cabinets mounted on Base
Stand
2" casters provide mobility
Logic® Cart
Accessories
Purifier® Logic® Biosafety Cabinet
Accessories
Ergonomic Chair• 6-way articulating seat• Removable armrests
Adjustable Footrest• Elevate feet and permits angle
repositioning while in use
Purifier® Logic® Biosafety Cabinet
Accessories
Support Shelves• Provide work surface to support remote
equipment• Epoxy-coated steel shelf mounts to left-side
frame of base stand
Laptop Computer Arm• Securely supports laptop computer• Articulating arm raises, lowers and pivots to
a steady position
Purifier® Logic® Agency Approvals
NSF Standard 49 Listed• With carbon post-filter and pre-filter
installed• With accessory shelves, hooks, and IV bar
in place
ETL, ETL-C and CE markAccessory Base stand tip tested by NSF
Working in Biological Safety Cabinets
Planning
Thoroughly understand procedures and equipment required before beginning work
and arrange for minimal disruptions
Have disinfectant and spill cleanup materials prepared
Working in Biological Safety Cabinets
Start Up
Turn off UV light, open sash to its proper height, and turn on cabinet lights and blower
Check grille for obstructions and let the cabinet operate for 5 minutes
Wash hands and arms thoroughly with disinfectant soap; wear a long sleeved lab coat and gloves
Use eye protection
Working in Biological Safety Cabinets
Wipe-Down
Wipe down all interior surfaces of the work area with a solution of 70% ethanol or other suitable disinfectant
Working in Biological Safety Cabinets
Loading
Load only the materials needed. Do not overload the cabinet or obstruct the grille. Keep large objects separated.
Lower the sash until it is in its proper position. Allow the unit to operate for 2 to 3 minutes to purge any airborne contaminants.
Working in Biological Safety Cabinets
Work Techniques
Segregate clean and contaminated materials. Arrange materials to minimize movement of contaminated materials into clean areas. Keep all contaminated material in the rear of the work area.
Avoid excessive movement of arms or materials through the front opening during operation.
Keep materials at least 4" inside the sash opening and perform all contaminated operations as far to the rear of the work area as possible.
Working in Biological Safety Cabinets
Work Techniques
Use proper aseptic technique.
Avoid techniques that disrupt airflow patterns in the cabinet, such as an open flame.
If there is a spill or splatter during use, all objects must be decontaminated before removal. Thoroughly
disinfect the interior surfaces of the cabinet while it is still in operation.
Working in Biological Safety Cabinets
Final Purging
After completing work, allow the cabinet to operate for 2 to 3 minutes undisturbed to purge airborne contaminants from the work area.
Working in Biological Safety Cabinets
Wipe-Down
Periodically lift the work surface and clean underneath it. Clean the towel catch.
Dispose of rubber gloves and have lab coat properly laundered. Wash arms and hands thoroughly with germicidal soap.
Wipe down all interior surfaces of the work area with a suitable disinfectant.
Working in Biological Safety Cabinets
Shutdown
Turn off the fluorescent light and cabinet blower, close the sash and turn on the UV light if appropriate.
Cabinet Maintenance
User Performed Maintenance
Replace fluorescent and UV lamps annuallyReset circuit breakersAirflow smoke testEstablish a smoke split
• Mark work surface where smoke split occurs• May use smoke stick or dry ice to determine
where split occurs • User should work behind smoke split
Cabinet Maintenance
Certification
A validation of cabinet integrity and performance
Performed by a qualified independent technician
Performed when the cabinet is newly installed, annually thereafter, or if the
cabinet is moved to a new location
Cabinet Maintenance
Certification Tests
HEPA filter leak testInflow and downflow verificationAirflow smoke pattern testElectrical safetyVibration, sound and lighting intensity tests (optional comfort tests)
Cabinet Maintenance
What to Look For in a Certifier
Accredited by an independent organization (NSF, AFCA, CETA)
Has attended cabinet manufacturer certifier training course
Membership in professional organizations
Experience with the cabinet type and model
References
Written estimates before work is begun
Operating Procedures
Myths, Lies and Gobbledygook
During operation, the flame of a burner is very disruptive to the airflow patterns of the cabinet, and may actually increase the dispersion of aerosols throughout the work area.
If the flame of the burner is too large, the excessive heat may melt the adhesive holding the HEPA filter together or literally burn holes in the filter media. An unattended burner may blow out. In a Type A1 or A2 cabinet, the gas may reachexplosive concentrations.
"I've got to use a Bunsen burner in my Biosafety Cabinet..."
Use alternate methods, such as electric incinerators, or disposable inoculating loops. Don’t flame bottle mouths; the work area of the biosafety cabinet should be a sterile environment, if used properly.
Operating Procedures
Myths, Lies and Gobbledygook
The Fume Hood is designed to remove noxious or toxic fumes and aerosols away from the operator. It should be constructed of materials that are inert to a wide variety of chemical agents.
The Biosafety Cabinet's primary purpose is to protect the operator, environment, and often the product from biohazardous contaminants. The biosafety cabinet and its HEPA filters are constructed of materials that are inert to the chemicals used in connection with biological research, but may be damaged by some of the more corrosive chemicals commonly used in fume hoods.
"I can use a biological safety cabinet as if it were a fume hood..."
Do NOT use a biosafety cabinet like a fume hood!
Operating Procedures
Myths, Lies and Gobbledygook
Wrong. The biosafety cabinet will provide Personnel and Product protection only if used properly. Aseptic technique must be practiced at all times while working in a Biohazard Cabinet.
"If I work in a biohazard cabinet, I don't have to be as careful with my technique."
Operating Procedures
Myths, Lies and Gobbledygook
Yes. The UV light is only an adjunct, to minimize contamination of the work area when the cabinet is not in use. UV light has practically no penetrating power, and will not kill microbes protected by dust, dirt, or organic material.
The best method to prevent contamination in the cabinet is regular decontamination of the work area surfaces, before and after each work session.
"If I use the UV light, do I need to decontaminate the work area?"
Operating Procedures
Myths, Lies and Gobbledygook
You shouldn’t. Large objects placed in the work area will impede the airflow, reducing the efficiency of the cabinet. Electrical appliances like centrifuges, blenders, etc. often have cooling fans that can disrupt the cabinet’s airflow. It’s better to use sealed safety cups in the centrifuge that are loaded and unloaded in the safety cabinet.
"Can I put a centrifuge in the biosafety cabinet? "
Operating Procedures
Myths, Lies and Gobbledygook
Not Necessarily. If you’re talking about protection from volatile toxic chemicals, yes; if your talking about protection from biohazardous aerosols, no.
Assuming the units being considered are both NSF listed, they were both subjected to the same biological challenge testing. Any claims of superior containment should be documented.
"A Type B cabinet will give better protection than a Type A."
Operating Procedures
Myths, Lies and Gobbledygook
In some applications, such as working with cytotoxic drugs, it is recommended the cabinet operate continuously, to prevent the escape of toxic material.
In most applications, operating the cabinet continuously will only shorten the life of the HEPA filters.
"All biohazard cabinets should operate continuously.”
Operating Procedures
Myths, Lies and Gobbledygook
Assuming you have an air-tight room, with no ventilation system, and no activity in it, then a recirculating Type A cabinet mightclean the air to Class 5 levels. Unfortunately, as soon as the user entered the room, particle-laden air would re-contaminate the room.
All this will really do is shorten the life of the HEPA filters.
"If I leave the biosafety cabinet operating continuously, it will clean the air to Class 5 conditions.”
Operating Procedures
Myths, Lies and Gobbledygook
Not necessarily, HEPA filters should be replaced when the pressure differential across them becomes excessive. The Purifier Logic Filter Life gauge will alert the user when the HEPA filters should be replaced.
The two biggest factors in filter life are the level of contaminants in the room air, and how many hours the cabinet operates.
Arbitrarily replacing HEPA filters on a timetable, without regard to loading may be unnecessarily expensive and wasteful.
"I’ve heard that you should replace HEPA filters annually.”
Labconco Corporation 8811 Prospect Ave.
Kansas City, MO 64132
1-800-821-5525 816-333-8811
www.labconco.com
www.labconco.comwww.labconco.com