2. POST TRANSCRIPTIONAL PROCESSING Primary transcript made by
RNA polymerase normally undergo further alteration, called post
transcriptional processing 3. POST TRANSCRIPTIONAL PROCESSING 4.
POST TRANSCRIPTIONAL PROCESSING 5. POST TRANSCRIPTIONAL PROCESSING
Different types of processing Cleavage of precursor of RNA Terminal
addition of nucleotides Base modification Splicing 6. POST
TRANSCRIPTIONAL PROCESSING Prokaryotic mRNA is not post
transcriptionally processed 7. POST TRANSCRIPTIONAL mRNA PROCESSING
mRNA processing 5 capping Addition of poly A tail Splicing 8. POST
TRANSCRIPTIONAL mRNA PROCESSING 5 capping Poly A tail 9. POST
TRANSCRIPTIONAL mRNA PROCESSING SPLICING SnRNPs 10. POST
TRANSCRIPTIONAL mRNA PROCESSING 11. POST TRANSCRIPTIONAL rRNA
PROCESSING PROKARYOTIC rRNA processing 12. POST TRANSCRIPTIONAL
rRNA PROCESSING EUKARYOTIC rRNA processing 13. POST TRANSCRIPTIONAL
tRNA PROCESSING tRNA PROCESSING Cleavage of a 5 leader sequence
Splicing to remove intron Replacement of 3terminal UU by CCA
Modification of several bases 14. POST TRANSCRIPTIONAL tRNA
PROCESSING 15. POST TRANSCRIPTIONAL tRNA PROCESSING 16. ALTERNATE
SPLICING 17. ALTERNATE SPLICING 18. RNA EDITING A change in the
base sequence of RNA after transcription by process other than RNA
splicing is called RNA editing 19. RNA EDITING ApoB-48 ApoB-100
ApoB gene Transcription CAA5 3 Unedited mRNA NH4 RNA editing by
deaminase 5 3 UAA Translation in intestine Translation in liver
4536 amino acids 2152 amino acids 20. INHIBITORS OF RNA SYNTHESIS
Binds to DNA Actinomycin D Binds to subunit of prokaryotic RNA
polymerase Rifampicin Inhibits eukaryotic RNA polymerase -Amanitin
21. REVERSE TRANSCRIPTION 22. GENETIC CODE The genetic code is the
system of nucleotide sequences of mRNA that designates particular
amino acid sequences in the process of translation 23. GENETIC CODE
The genetic code is in the form of CODONS. CODONS are a group of
three adjacent bases that specify the amino acids of protein 24.
GENETIC CODE 25. CHARACTERISTICS OF GENETIC CODE Number of codons
Stop or nonsense codons Code is degenerate but unambiguous The code
is almost universal The code is non- overlapping 26. Codon-
anticodon recognition 27. Codon- anticodon recognition 28. WOBBLE
HYPOTHESIS The first 2 bases of codons are same, third is
different, wobble Wobble allows some tRNAs to recognize more than
one codon
