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Parasitology
Malaria
JMJ 1
JMJ
O Great St. Joseph of Cupertino who while on earth
did obtain from God the grace to be asked at your
examination only the questions you knew, obtain for
me a like favors in the examinations for which I am
now preparing. In return I promise to make you
known and cause you to be invoked.
Through Christ our Lord.
St. Joseph of Cupertino, Pray for us.
Amen.
Plasmodium vivax – Ring trophozoite
Red cells enlarged
Trippling (Schuffner’s dot) in the RBC cytoplasm
Signet ring appearance (ring is coarse and large)
JMJ 2
N- Nucleus/ chromatin R – Normal RBC V- Vacuole P- Parasitic cytoplasm S – Schuffner’s dots
Plasmodium vivax – Amoeboid trophozoite
Red cells enlarged, strilling more marked
Vacuole starts disappearing.
Parasite nucleus is NOT divided
Parasite cytoplasm developed pseudopodia
(Parasite looks like an amoeba)
Few granules of malaria pigment may be present
JMJ 3
N- Nucleus/ chromatin R – Normal RBC M- Malaria pigment P- Pseudopodia S – Schuffner’s dots
Plasmodium vivax – Immature schizont
Red cells enlarged,
Schuffner’s dots are more marked
Parasitic nuclear fragments are still dividing
Malaria pigment ++
JMJ 4
N- Nucleus/ chromatin R – Normal RBC M- Malaria pigment S – Schuffner’s dots
Immature schizont
JMJ 5
Plasmodium vivax – Mature schizont
Red cells enlarged,
Schuffner’s dots are more marked
Parasitic nuclear is divided into 12-24 segments
Parasitic cytoplasm is compact and collected around nuclear fragment (Pink/red)
Malaria pigment +++
JMJ 6
N- Daughter nuclei R – Normal RBC M- Malaria pigment S – Schuffner’s dots
Mature Schizont
JMJ 7
Plasmodium vivax –mature schizont
JMJ 8
Plasmodium vivax – Male gametocyte/ microgametocyte Red cells enlarged,
Gametocyte is large, round and entirely fills the RBC
Parasitic nucleus is not distinct but dispersed and located centrally
Parasitic cytoplasm is pinkish in colour (Due to dispersed nucleus)
Malaria pigment (Yellowish brown) is prominent
Strippling is visible
No vacuole
JMJ 9
N- Nuclei M- Malaria pigment P- Parasitic cytoplasm
Micro gametocytes
JMJ 10
Plasmodium vivax – Female gametocyte/ macrogametocyte Red cells enlarged,
Gametocyte is large, round and entirely fills the RBC
Parasitic nucleus is very distinct and pushed to a side
Parasitic cytoplasm is bluish in colour
Malaria pigment (Yellowish brown) is prominent
Strippling is visible
JMJ 11
N- Nuclei M- Malaria pigment P- Parasitic cytoplasm
Plasmodium vivax – Female gametocyte/ macrogametocyte
JMJ 12
Plasmodium vivax – Female gametocyte/ macrogametocyte
JMJ 13
JMJ 14
Vivax
JMJ 15
Ring trophozoite Amoeboic trophozoite
Vivax
JMJ 16
Vivax
JMJ 17
Mature schizont
Vivax
JMJ 18
Plasmodium falciparum
JMJ 19
Accole form
A- cytoplasm ring
B- chromatin
C- Ring with 2 chromatin dots
JMJ 20
Plasmodium falciparum – Ring trophozoite
Red cell is NOT enlarged
Strippling is seen as coarse maurer’s dots or clefts in the RBC cytoplasm (Few dots compared to P.vivax)
Ring is very delicate and small
Multiple invasion (Polyparasitism)
Double nuclear phenomenon
Applique form (Accole form)- chromatin and cytoplasm of parasire is applied to the membrane of the RBC
JMJ 21
A- Ring stage B- Double nuclear phenomenon C- Applique form D- Multiple invasion M- Maurer’s dots R- Normal RBC
Ring trophazites
JMJ 22
Plasmodium Falciparum – Mature schizont
Red cell is not enlarged,
No strippling
Parasitic nucleus is divided
Parasitic cytoplasm is compact and collected around nuclear fragments
Malaria pigment appears inside the RBC as a clumped black body
JMJ 23
N- Daughter nuclei R – Normal RBC M- Malaria pigment
JMJ 24
Plasmodium falciparum – Male gametocyte/ microgametocyte Gametocyte is sausage shaped with blunt
rounded ends
Parasitic nucleus (pink / red ) is not distinct but diffused.
Parasitic cytoplasm is pinkish in colour.(Due to diffused nucleus ).
Malaria pigment (jet black ) is scattered inside.
No vacuole
JMJ 25
N- Nuclei M- Malaria pigment P- Parasitic cytoplasm
Microgamete
JMJ 26
Microgamete
JMJ 27
Plasmodium falciparum – Female gametocyte/ macrogametocyte Found inside RBC .
Gametocyte is cresentic in shape with pointed ends.
Parasitic nucleus (pink /red ) is distinct, well defined and located at the centre.
Parasitic cytoplasm is bluish in colour.
Malaria pigment (jet black ) is scattered inside.
No vacuole.
JMJ 28
N- Nuclei M- Malaria pigment P- Parasitic cytoplasm
Macrogamete
JMJ 29
Gametocytes
JMJ 30
Oocysts in mosquito gut
JMJ 31
Plasmodium vivax- Ookinate
JMJ 32
Thank You!
JMJ 33