Microbiology Practical I

The following slides were contributed by your fellow BIO 251 students…

Use side “” key to view slides…

Lab 1: Eukaryotes and Microscopy

Know the parts of the microscope…

Lab 1: Eukaryotes and Microscopy

Paramecium caudatum

Lab 1: Eukaryotes and Microscopy

Euglena gracilis

Lab 1: Eukaryotes and Microscopy

Amoeba proteus

Lab 1: Eukaryotes and Microscopy

Fungus – Saccharomyces cerevisiae

Lab 1: Eukaryotes and Microscopy

Cyanobacteria ~ Nostoc

~Bacteria slides~

Micrococcus luteus

~Bacteria Slides~

Bacillus subtilis

~Bacteria Slides~

Bacillus anthracis

~Bacteria Slides~

Rhodospirillum rubrumSorry not in focus…

~Bacteria Slides~

See other file for Gram Stain, Endospore, Acidfast, and Capsule Staining…

[ Adobe Acrobat of Gram and Endospore ]

Some Endospore Staining ~

Streak Plate:

Know colony characteristics (color, elevation, margin, etc.)

Spread Plate vs. Streak Plate:

Streak Plate:

Spread Plate:Mixed – Bacillus subtilis & Serratia marcescens

Practical I Unknown Tests:

~ Simple Stain~ Gram Stain~ Endospore Stain~ Acid-Fast Stain (Demo)~ Capsule Stain (Demo)~ Motility (Hanging Drop, Wet Mount, Soft Agar Deep)

~ Obligate Aerobe, Facultative Anaerobe, Aerotolerant Anaerobes, Microaerophiles (Gas Pak, Eugon Agar, Thioglycollate Broth)

Gas Pak Anaerobic vs. Aerobic:

Dilution Problems:

Dilution Problems:

Bacterial Growth Curve:Make sure you know how to:

Use a spectrophotometer to measure absorbance.

Produce a log graph of the calculations.

Calculate generation time from a growth curve.