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Toxicological Risk Assessment

An initiative of

24 October 2013 - UCL - Brussels (Woluwe)

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Laboratory of Cancerology and Experimental Toxicology - Faculty of Pharmacy

Université Libre de Bruxelles

Pr Véronique Mathieu MD, Ph.D.

Associate Professor

Fields of expertise

• Natural (derived) compounds

• Apoptosis-resistance

• migration- invasion

• Anti-cancer activities

Industrial applications fields

• Discovery of new chemical entities to combat apoptotic

resistant cancers like glioma, melanoma, non-small cell lung

carcinoma….

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Fields of expertise and industrial targets

• Galectin-1

• Actin

• Gene silencing: siRNA

• Videomiscroscopy

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Applied research projects: example of galectin-1

Pub. No.: WO/2006/108474 International Application No.: PCT/EP2006/002170

Publication Date:

19.10.2006

International Filing Date:

09.03.2006

Chapter 2 Demand Filed: 09.02.2007

IPC: C12N 15/11 (2006.01), A61K 48/00 (2006.01)

Applicants: UNIVERSITE LIBRE DE BRUXELLES [BE/BE]; Avenue F.D. Roosevelt 50, CP 161, B-1050 Brussels (BE) (All Except US). UNIVERSITE CATHOLIQUE DE LOUVAIN [BE/BE]; Place De L'université 1, B-1348 Louvain-la-Neuve (BE) (All Except US). CAMBY, Isabelle [BE/BE]; Rue Ferrer 22, B-1480 Tubize (BE) (US Only). HENRIET, Patrick [BE/BE]; Rue De La Briqueterie 17, B-1400 Nivelles (BE) (US Only). LEFRANC, Florence [BE/BE]; H. Consciencestraat 34, B-1600 Sint-Pieters-Leeuw (BE) (US Only). COURTOY, Pierre [BE/BE]; Avenue De Gomrée 4, B-1150 Brussels (BE) (US Only). KISS, Robert [BE/BE]; H. Consciencestraat 34, B-1600 Sint-Pieters-Leeuw (BE) (US Only).

Inventors: CAMBY, Isabelle; Rue Ferrer 22, B-1480 Tubize (BE). HENRIET, Patrick; Rue De La Briqueterie 17, B-1400 Nivelles (BE). LEFRANC, Florence; H. Consciencestraat 34, B-1600 Sint-Pieters-Leeuw (BE). COURTOY, Pierre; Avenue De Gomrée 4, B-1150 Brussels (BE). KISS, Robert; H. Consciencestraat 34, B-1600 Sint-Pieters-Leeuw (BE).

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Special equipments

Examples

• Culture rooms • Flow cytometry (3 colors) • Fluorescent microscope • ELISA • (RT)-PCR, qPCR • WB • …

• Quantitative videomicroscopy • Animal facilities and cancer aggressive models

16 videomicoscopic devices

> 20 years of expertise

Computer-Assisted Phase-Contrast Microscopy

(Quantitative Videomicroscopy)

Debeir O et al. IEEE Trans Med Imaging 2005 SKMEL28 Melanoma Cells / Control

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Soft agar test

MTT colorimetric test

The cells are cultured for 3 days in presence of the compound

Cytotoxic ?

Cytostatic ?

Anti-adhesive ?

Computer assisted videomicroscopy

Dumont P et al., Neoplasia 2007

x 250

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Examples • MTT assay on normal and cancer cells

• Quantitative videomicroscopy experiment

• Cell cycle and / or apoptosis analysis

• ROS production analysis

• Migration assays (boyden chambers, quantitative scratch

wound assay)

• Actin or tubulin cytoskeleton analysis (fluorescence)

• Mechanism of action deciphering (adapted to your project)

• Maximal tolerated dose in vivo in conventional mice

• Anti-cancer activity on cancer bearing mice

• …

Services provided to companies

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• PhD training

• Post-Doctoral fellowship

Training proposals to companies

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Contacts

Université Libre de Bruxelles - Faculty of Pharmacy Laboratory of Cancerology and Experimental Toxicology

Boulevard du Triomphe

Campus de la Plaine, CP205/1 Building BC

1050 Brussels http://www.ulb.ac.be/rech/inventaire/unites/ULB646.html

Pr Véronique Mathieu

Associate Professor

vemathie@ulb.ac.be +32 478 317 388

Pr Jean-Michel Kauffmann

Head of the Laboratory

jmkauf@ulb.ac.be +32 2 650 52 15