The The Isotonix® Advantage Advantage

Mark Lange, Ph.D.

Director, Quality Control

Market America

OVERVIEWOVERVIEW Understanding Your Digestive Understanding Your Digestive

SystemSystem Transporting Nutrients into CellsTransporting Nutrients into Cells The Isotonix Delivery SystemThe Isotonix Delivery System Isotonix Advantage #1,2,3…..Isotonix Advantage #1,2,3…..

STOMACHSTOMACH

Secretes 5 substances mainly:Secretes 5 substances mainly: Water – diluent Water – diluent Chloride ionsChloride ions Hydrogen ionsHydrogen ions Pepsin – proteolytic enzyme (proteins)Pepsin – proteolytic enzyme (proteins) Intrinsic factor – vitamin B-12 binding Intrinsic factor – vitamin B-12 binding

protein released by parietal cellsprotein released by parietal cells

Hydrochloric acid Hydrochloric acid HCl = pH 1.0 – 2.8HCl = pH 1.0 – 2.8

Isotonix Advantage #1

Nutrients are not exposed to acidic environment of stomach, where they would otherwise degrade.

Customer money is not wasted.

Isotonix Advantage #2

Reduced delivery time.

Example: B vitamins for energy

Minutes to uptake – not hours

SMALL INTESTINESMALL INTESTINE ACIDIC FOOD SOLUTIONACIDIC FOOD SOLUTION

stimulates pancreas to release stimulates pancreas to release numerous digestive enzymes in a numerous digestive enzymes in a sodium bicarbonate solutionsodium bicarbonate solution

NEUTRALIZATIONNEUTRALIZATION pH of food pH of food solution changed from acidic to solution changed from acidic to nearly neutral nearly neutral

SMALL INTESTINESMALL INTESTINE BILE is a product of the liver, BILE is a product of the liver,

collected in the gall bladder, and collected in the gall bladder, and secreted onto the food solution.secreted onto the food solution.

Contains surfactants that disperse Contains surfactants that disperse fat.fat.

Lipases act on triglycerides, Lipases act on triglycerides, releasing fatty acids.releasing fatty acids.

Isotonix Advantage #3

Carbon dioxide created by the effervescent reaction reduces the thickness and viscosity of the mucus layer of the intestinal tract, promoting absorption into the cells.

Pharm. Res. 1998, vol 15, pp 925-930. “Mechanistic studies on effervescent-induced permeability enhancement.”

FOLDS FOLDS IN THE SMALL INTESTINEIN THE SMALL INTESTINE

Villi

Mucosa

Sub -Mucosa

The Cell Membrane

Cell Membrane Barriers

Lipid bi-layers are impermeable to most essential molecules and ions.

Oxygen, Water, & Carbon Dioxide can freely pass.

Many nutrients cannot freely pass.

Movement through Differences in Concentration

Diffusion is moving molecules from a region of higher concentration to a region of lower concentration.

Simple Diffusion

Higher concentrations move nutrients into cells

Membrane (lipid bi-layer)

Isotonix Advantage #4

Favorable concentration gradient for absorption of some nutrients. Example: Calcium/Magnesium

Nutrients are delivered to the small intestine in a concentrated solution.

Facilitated Diffusion

Takes place through channels embedded in the membrane.

Example: Fructose moved into cell by facilitated diffusion.

Active Transport Pumping of molecules or ions

through a membrane into a region of higher concentration. It requires a trans-membrane protein and energy input.

Example: Amino Acids, ATP, Sodium, Potassium moved into cell by Active Transport.

SMALL INTESTINESMALL INTESTINE Absorption of nutrients occurs Absorption of nutrients occurs

throughout entire length of small throughout entire length of small intestine (90 – 95% efficient)intestine (90 – 95% efficient)

Calcium absorption occurs as late as Calcium absorption occurs as late as ileo-cecal juncture (appendix) ileo-cecal juncture (appendix) transport into cells via calcium channelstransport into cells via calcium channels

Under neutral pH, intrinsic factor binds Under neutral pH, intrinsic factor binds to vitamin B-12 to form a complexto vitamin B-12 to form a complex

SMALL INTESTINESMALL INTESTINE Intrinsic factor/vitamin B-12 complex Intrinsic factor/vitamin B-12 complex

binds to receptors (specialized binds to receptors (specialized docking structures) on intestinal docking structures) on intestinal membranesmembranes

Last 1/3Last 1/3rdrd of small intestine is main of small intestine is main absorptive siteabsorptive site

COLONCOLON Most nutrients will have been absorbed by the Most nutrients will have been absorbed by the

time they reach the large intestine —an time they reach the large intestine —an additional 3-5% possible.additional 3-5% possible.

Major functions of colon:Major functions of colon: Site of bacterial cell growth and residenceSite of bacterial cell growth and residence An estimated 300-400 species or strains of An estimated 300-400 species or strains of

bacteria continue digestion of waste bacteria continue digestion of waste productsproducts

Regulates water in bodyRegulates water in body Some vitamins produced in low amounts by Some vitamins produced in low amounts by

bacteria and absorbedbacteria and absorbed

COLONCOLONBacteria adjust the pH of the colon into Bacteria adjust the pH of the colon into the acidic range – prevents overgrowth the acidic range – prevents overgrowth of harmful bacteria.of harmful bacteria.

Continued growth of friendly bacteria Continued growth of friendly bacteria help maintain healthy balance.help maintain healthy balance.

What does “isotonic” What does “isotonic” mean?mean?

ISO = SAMEISO = SAME

TONIC = Tension or PressureTONIC = Tension or Pressure

An Isotonic Cell is a Happy Cell.An Isotonic Cell is a Happy Cell.

Cells act like little bags of salty water…..

The movement of water in and out of a cell is called “Osmosis.”

Cells want the same concentration

of dissolved particles (salt) on both sides of the membrane.

ISOTONIC

(NORMAL)

HYPOTONIC (WATER

GOING IN)

HYPERTONIC (WATER GOING

OUT)

Isotonix Advantage #5

Isotonic solutions are the optimal delivery system.

Isotonix products work with – not against – normal body physiology.

Composition of Composition of Isotonix Delivery System Delivery System Active ingredients:Active ingredients: vitamins, vitamins,

minerals, oligomeric minerals, oligomeric proanthocyanidins (OPC), proanthocyanidins (OPC), flavonoids, coenzyme Q10, etc.flavonoids, coenzyme Q10, etc.

Base ingredients:Base ingredients: fructose, fructose, glucose, citric acid, malic acid, glucose, citric acid, malic acid, calcium sulfate, maltodextrin, calcium sulfate, maltodextrin, silica, potassium bicarbonatesilica, potassium bicarbonate

Composition of Composition of Isotonix Delivery System Delivery System Glucose and fructoseGlucose and fructose: :

Sweet-tasting simple sugarsSweet-tasting simple sugars Add significant osmotic pressureAdd significant osmotic pressure Caloric value (Caloric value (~ 8 cal/serving)~ 8 cal/serving) Fructose: principle sugar in formula Fructose: principle sugar in formula

scores 20 out of 100 on glycemic scores 20 out of 100 on glycemic index (i.e., relatively low value)index (i.e., relatively low value)

Composition of Composition of Isotonix Delivery System Delivery System

Citric acid and malic acidCitric acid and malic acid: : Adjust pH to acid range (about 3.5 – Adjust pH to acid range (about 3.5 –

5.0)5.0) Adjust taste to “citrus” flavorAdjust taste to “citrus” flavor Minor osmotic pressure adjustmentMinor osmotic pressure adjustment

Composition of Composition of Isotonix Delivery Isotonix Delivery SystemSystem

Calcium sulfateCalcium sulfate: : Desiccant (takes up moisture in Desiccant (takes up moisture in

powder) / free-flow agent powder) / free-flow agent Minor osmotic pressure adjustmentMinor osmotic pressure adjustment Minor nutritive valueMinor nutritive value

Composition of Composition of Isotonix Delivery Isotonix Delivery SystemSystem

MaltodextrinMaltodextrin: : Glucose polymer has minor caloric Glucose polymer has minor caloric

valuevalue Powder — bulking agentPowder — bulking agent Prevents citric acid from reacting with Prevents citric acid from reacting with

potassium bicarbonate and waterpotassium bicarbonate and water Thickening and suspending agent in Thickening and suspending agent in

solutionsolution

Composition of Composition of Isotonix Delivery Isotonix Delivery SystemSystem

Potassium bicarbonatePotassium bicarbonate: : Potassium and bicarbonate ions have Potassium and bicarbonate ions have

significant osmotic pressure significant osmotic pressure Helps adjust and buffer pHHelps adjust and buffer pH Release of CORelease of CO2 2 stirs liquidstirs liquid

Isotonix Advantage #6 Isotonix offers an alternative to

pills.

Survey shows 40% of American adults have experienced difficulty swallowing pills

The survey of 679 adults (513 ages 18-64, 166 age 65 and older) was funded by

the US affiliate of Germany's Schwarz Pharma.

Isotonix Advantage #7 People with stomach and esophageal

challenges benefit from Isotonix delivery. Acid reflux sufferers Bariatric patients Hiatal Hernia (stomach protrudes up

through diaphragm) People who have trouble swallowing Elderly (reduced acid production)

Conclusion

Isotonix® product line offers customers a wide selection of nutrient combinations with the advantages of isotonic delivery.

Isotonix® is the logical choice of those seeking fast and optimal nutrient uptake.