1

IN-VITRO EVALUATION OF ANTIOXIDANT AND ANTI-DIABETIC ACTIVITY OF KIGELIA AFRICANA

Sreejith P S (15MSB0045), Majesh Mathew (15MSM0070).

Research Guide: Dr. Devi Rajeswari V

Assistant Professor (Sr),School of Biosciences and Technology,

VIT University, Vellore-14.

13SETMSB1300180

ABSTRACT

• Aqueous leaf extract of medicinal plant Kigelia africana were screened for :

Phytochemical analysis

DPPH radical scavenging activity

Total Antioxidant activity

Hydrogen peroxide scavenging activity

In-vitro Anti-diabetic acitivity

Non enzymatic glycosylation hemoglobin assay

Inhibition of alpha amylase assay

Glucose uptake by yeast cells

INTRODUCTION• Diabetes – A gathering of metabolic illnesses described by hyperglycemia coming about because

of imperfections in insulin discharge, insulin activity, or both.

Causes: Diabetic ketoacidosis, coronary illness, stroke, unending kidney disappointment, foot

ulcers and harm to eyes.

• Antioxidants are involved in prevention of cellular damage- Cancer, aging and variety of

diseases.

• Medicinal plants assume a vital part in the administration of diabetes mellitus particularly in

creating nations where assets are pitiful.

• The antioxidant activity of Kigelia africana was evaluated by measuring reducing ability, free

radical scavenging activity by DPPH and hydrogen peroxide methods.   

4

METHODOLOGY

Sample collection Extraction process Phytochemical analysis

Antioxidant activityAnti –diabetic activity

Sample collection

Kigelia africana leaves

Extraction process

RESULTS AND DISCUSSION:

1. Phytochemical analysis

• Qualitative analysis

S.no Phytochemical tests

Qualitative analysis

Results

1. Alkaloids Wagner’s test - 2. Carbohydrates Benedict,s test - 3. Proteins and

aminoacid Millions test -

4. Glycoside Borntrager’s test ++ 5. Saponins Foam test +++ 6. Steroids and sterols Salkowski test ++ 7. Phenolic Lead acetate test +++ 8. Tannins Ferric chloride test +++

9. Flavanoids Sodium hydroxide test

++

2. Antioxidant activity:

DPPH radical scavenging activity

3. Total Antioxidant activity:

4. Hydrogen peroxide scavenging activity:

25 50 75 1000

10203040506070

Hydrogen Peroxide

Aqueous extractAscorbic acid

Concentration in µg/mlAb

sorb

ance

25 50 75 10001020304050607080

Total antioxidant activity

Aqueous extractAscorbic acid

Concentration in µg/ml

Abso

rban

ce

• 6. In-vitro anti diabetic activity• Non enzymatic glycosylation hemoglobin assay

200 400 600 800 10000

1020304050607080

Non Enzymatic Glycosylation of Haemoglobin Assay

tocopherolaqueous

concentration (µg/ml)

%in

hibi

tion

• Inhibition of alpha amylase assay

200 400 600 800 1000010203040506070

Alpha Amylase Inhibition

acarboseaqueous

Concentration (µg/ml)%

inhi

bitio

n

• Glucose uptake by yeast cells

40 80 120 160 20001020304050607080

Glucose uptake by yeast cell (10mM)

acarboseaqueous

Concentration (µg/ml)

% o

f upt

ake

40 80 120 160 2000

1020304050607080

Glucose uptake by yeast cells (5mM)

acarboseaqueous

Concentration (µg/ml)

% o

f upt

ake

Conclusion:

As indicated by all tried method, the cell reinforcement action of Kigelia africana was contrasted and that of Ascorbic

acid, which is an actually happening natural compound with cancer prevention agent movement. All in all, the present

study reports that Kigelia africana concentrate can be a decent premise of cancer prevention agent movement and it

likewise can hinder glucose dispersion, α–amylase action and to enhance glucose take-up action by yeast cells utilizing

as a part of vitro models. The confirmation recommend that the plant might be helpful in dynamic administration of

Type2 Diabetes mellitus. A great deal more examinations are required to clarify whether in-vitro impacts speak to

remedial probability in-vivo models for refining glycemic control in Type 2 Diabetes.

Reference

1. Diagnosis and Classification of Diabetes Mellitus, American Diabetes Association. Diabetes Care 2010

Jan; 33(Supplement 1): S62-S69.

2. Medicinal plants in therapy, Norman R. Farnsworth, Olayiwola Akerele, Audrey S. Bingel, Djaja D. Soejarto, and 

Zhengang Guo. Bull World Health Organ. 1985; 63(6): 965–981.

3. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity, Molyneux, P.

Songklanakarin J. Sci. Technol., 2004, 26(2) : 211-219

4. Medicinal plants with potential antidiabetic activity - A review of ten years of herbal medicine research (1990-

2000), Mohamed Bnouham*, Abderrahim Ziyyat, Hassane Mekhfi, Abdelhafid Tahri, Abdelkhaleq Legssyer. Int J

Diabetes & Metabolism (2006) 14: 1-25

5. Alfa-glucosidase-inhibiting activity of some Mexican plants used in the treatment of type 2 diabetes, Adolfo

Andrade-Cetto a,∗, Jaime Becerra-Jimenez ´ a, Rene C´ ardenas-V ´ azquez. Journal of Ethnopharmacology 116

(2008) 27–32

6. R. Sicree, J. Shaw and P. Zimmet., The Global Burden of Diabetes: Diabetes and Impaired Glucose Tolerance:

Prevalence and Projections, Diabetes Atlas, International Diabetes Federation, Belgium, 2003, pp. 27-6.