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Christiane’s Super Crazy Complicated Difficult Challenging Super Crazy
ProfessionalGuide to DNA Extraction
Christiane’s Guide to DNA Extraction
1. Spit in a cup 2. Add a drop of soap 3. Add a pinch of salt and mix4. Add three spoonfuls of alcohol
Mouth
Saliva
Soap
DNA
DNA
Water
Phosphate Backbone (DNA)
Phosphate Backbone (DNA)
Salt
Alcohol
Christiane’s Guide to DNA Extraction
• Spit in a cup (~1mL of saliva)• Add a drop of soap and mix
You do not need a lot• Add a pinch of salt and mix• Add three spoonfuls of alcohol
– Cover the top of the container– Swirl the cup, but DO NOT mix the contents with a spoon,
otherwise you will break up the DNA into fragments– Allow for the contents to settle– If DNA is not visible after a few minutes, add another
spoonful of alcohol