CYCLOPHOSPHAMIDE

Ferdinand F. Fatiga, RMT

CYCLOPHOSPHAMIDE

Used in the treatment of WBC cancers

(AML/ALL) and Lymphoma

Cell Cycle – Non specific

Alkylating agent Adds Alkyl group (CnH2n+1) to DNA

CYCLOPHOSPHAMIDE

Mechanism of Action

• the main effect of cyclophosphamide is due to its metabolite Phosphoramide mustard

• Phosphoramide mustard forms DNA crosslinks between (interstrand crosslinkages) and within (intrastrand crosslinkages) DNA strands at guanine N-7 position, this eventually leads to cell death

CYCLOPHOSPHAMIDE Activation

CYCLOPHOSPHAMIDE

CONCLUSION

Expression of genes that code for Cytochrome P450 (Oxidase Enzyme) and ALDH enzymes affects the sensitivity of Cancer cells to Cyclophosphamide since these enzymes affect the metabolism of the prodrug (Cyclophosphamide) to its active Phosphoramide mustard metabolite.