doc No. RdtB000003, Revision 1.01, dtd 03 January 2011.

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ubio quickVet RaPId canine Parvovirus

antigen lateral Flow Immuno-assay

ubio Biotechnology systems Pvt. ltd.

Ph: +91-484-2532966, +91-9446438678

web: http://www.ubio.in email: contact@ubio.in

ubio quickVet canine Parvovirus antigen Rapid test Kit is a qualitative immunochromatographic assay

for the detection of Parvovirus cPV2 antigen in faeces in just 5-10 minutes.

PaRVoVIRus cPV2

cPV2, is a virus that attacks and destroys cells in lymph nodes, intestinal crypts and bone marrow. In-

fected dogs excrete the virus along with faeces. dogs are infected by oral contact with infected material.

the virus causes inflammation of intestines in adult dogs with occasional mortality and fatal cardiac ar-

rests in pups. Infected dogs show symptoms within 5-10 days.

the major clinical features of the disease are sudden persistant vomiting, refusal to eat or drink, lethargy,

fever, watery diaorrhea with blood, rapid dehydration and weight loss. untreated animal dies within 48-

72 hours, mainly due to dehydration, secondary infections and endotoxemia.

cPV2 infection is not transmissible to humans.

assay oVeRVIew

ubio quickVet canine Parvovirus antigen Rapid test is a lateral flow

assay designed so that even a technician with limited expertise can

perform the test easily. the veterinarian or the technician collects

a sample of faeces using the swab provided with the test. the swab

is inserted into the assay diluent vial and agitated so that the sample

is extracted into the diluent. three drops of this diluent is then

added to the sample hole on the test card using the dropper pro-

vided with the test.

the results can be read in just 5-10 minutes - the control line,

marked c, should be always appear if the test procedure is per-

formed properly and the test reagents are working. a red test line,

marked t, will be visible if canine Parvovirus cPV2 antigen is pres-

ent in faeces.

the test detects cPV2, cPV2a, cPV2b and cPV2c variants of the

virus.

assay comPoNeNts aNd PRINcIPle

ubio quickVet RaPId canine Parvovirus antigen lateral Flow Immuno-assay is a sandwich assay that

consists of the following components:

• sample pad

an absorbent pad onto which the test sample is applied.

• conjugate pad

this pad contains cPV2 antibody conjugated to red-coloured colloidal gold coloured parti-

cles.

Positive, Negative and

Invalid Results

doc No. RdtB000003, Revision 1.01, dtd 03 January 2011.

a printed copy of the document is uncontrolled

these components are fixed on an inert plastic backing ma-

terial mounted inside a plastic cassette with properly sized

ports for adding the sample and for reading the result. the

sample addition port is aligned along the top of the sample

pad and the result port is aligned along the reaction mem-

brane showing the test and capture zones.

the sample that is added to the sample port migrates from

the sample pad along the conjugate pad where any cPV2

antigen present in the sample will bind to the conjugated

antibody. the sample then continues to migrate across the

membrane until it reaches the capture zone where the anti-

gen-conjugate complex will bind to the immobilised cPV2

antibody producing a visible line on the membrane. If cPV2

antigen is not present in the sample, no reaction occurs in

the capture zone and no test line is formed. the sample

then migrates further along the strip until it reaches the

control zone, where excess antibody conjugate will bind to

the immobilized cPV2 antigen and produce a second visi-

ble line on the membrane. this control line indicates that

the sample has migrated across the membrane as intended.

• Reaction membrane

a hydrophobic nitrocellulose membrane through which the test sample can flow.

• capture/test zone

an area on the reaction membrane onto which cPV2 antibody is immobilized in a line.

• control zone

an area on the reaction membrane onto which cPV2 antigen is immobilized in a line.

• wick or waste reservoir

an absorbent pad designed to draw the sample across the reaction membrane by capillary

action and collect it.

assay PaRameteRs

Sensitivity

98.5%

Specificity

98.0%

data based on comparison with

industry-standard elIsa tests. Positive

result confirms diagnosis, but negative

result does not exclude parvovirosis as

virus excretion in the faeces is intense

but short. False positives may occur if

the test is performed within 12 days of

vaccination.