投影片 1

Stem cell research Stem cell research

laboratory laboratory

and tissue bank and tissue bank

Coordinator: ： Su-Peng YehTel ： +886-4-22052121ext1513

資料更新日期： 98.7.2

Introduction Supervisor: Dr. Su-Peng Yeh

Dr. Su-Peng Yeh graduated from Taipei Medical College in 1991 and received resident training in internal medicine at Veteran General Hospital-Kaohsiung. He completed his fellowship training in Medical Oncology and Hematology at Veteran General Hospital-Kaohsiung and National Chan-Gon University Hospital in 1998. He was recruited to be an attending physician in Division of Hematology and Oncology at China Medical University Hospital in Dec. 1998. Dr. Yeh is an experienced specialist in medical oncology, haematology, and blood and bone marrow transplantation. He leads the team of hematopoietic stem cell transplantation in adults at China Medical University Hospital since 2001 and is the director of Stem Cell Research Laboratory and Tissue Bank since 2007. Because of his active leadership as well as the well-integrated team work, China Medical University Hospital becomes the referral center of hematopoietic stem cell transplantation at central Taiwan in the recent years. In addition to the clinical services, Dr. Yeh also actively leads the Stem Cell Research Lab. in doing research. The major interests of research are transplant immunology, mesenchymal stem cell, and cancer/leukemic stem cell. He has published many papers in peer review journals such as leukemia, British Journal of Haematology, and Bone Marrow Transplantation. He also presented the research results in the major international conference of haematology and transplantation such as annual meeting of ASH, ESH, and EBMT. He is now an assistant professor of medicine at China Medical University Hospital.

Introduction

SUPERVISOR PROFILE(主管簡歷 )

Photo(生活照或大頭照 )

Speciality(專科 ) Hematology, Oncology

Name (姓名 ) Su-Peng Yeh

Gender (性別 ) Male

Language Spoken(語言 ) 1.Chinese, 2.English

Qualification (Qualification ( 學歷、資格證明學歷、資格證明 )) YearYear

1. MD, School of Medicine, Taipei Medical College 1991

2. Fellowship, Division of Hematology/Oncology, Veterans General Hospital-Kaohsiung 1996-1998

3. Domestic:Certified medical oncologist / Hematologist 1998 / 1999

4. Visiting Scientist, University of Texas, MD Anderson Cancer Center 2006-2007

Excellent Practice (Excellent Practice ( 特殊專長特殊專長 ))

1. Cancer chemotherapy and high dose chemotherapy

2. Autologous and allogeneic hematopoietic stem cell transplantation

3. Transplant immunology and mesenchymal stem cell

Experience (Experience ( 經歷經歷 )) YearYear

1.Resisdent, Department of Medicine, Veterans General Hospital-Kaohsiung 1993-1996

2.Attending physician, Division of Hematology/Oncology, China Medical University Hospital Since 1998

3. Director of stem cell research laboratory and tissue bank; China Medical University Hospital Since 2007

Award (Award (得獎得獎 )) YearYear

1.

2.

3.

Publication (Publication ( 論文發表論文發表 ))

Essay(論文名稱 ) Journal (刊名 ) Date (出版日期 )

Speciality(專科 )Pediatric Resident 、 Pediatric Hematology/Oncology

Name (姓名 ) Kang-Hsi Wu, M.D.

Gender (性別 ) male

Language Spoken(語言 )

Chinese

Qualification (學歷、資格證明 ) Year

1. China Medical College, Taichung, Taiwan, M.D. 1994

2. Pediatric Resident, 1st, 2nd and 3rd year & Chief Resident 1year - China Medical College Hospital

1996

3. Fellow, Pediatric Hematology/Oncology – The China Medical University Hospital and the National

Taiwan University Hospital1999

4. Chief of Pediatric Hematology/Oncology Division, China Medical University Hospital

2002~present

Excellent Practice (特殊專長 )

1. Pediatrics

2. Pediatric Hematology/Oncology

3. Hematopoietic stem cell transplantation

Experience (經歷 ) Year

1. Chief of Pediatric Hematology/Oncology ward, China Medical University Hospital

2002~present

2. Staff Pediatrcian: China Medical University Hospital 2002~present

3. Adviser of blood bank, China Medical University Hospita

2002~present

4. Adviser of Stem cell research labaratory, China Medical University Hospital,

2002~present

5. Taiwan Pediatric Society 2005~present

6. Consultant of Taiwan Thalassemia Society 2005~present

7. Consultant of Make-A-Wish Foundation 2006~present

8. Taiwan Society for Stem Cell Research 2006~present

9. Taiwan Society of Blood Transfusion 2007~present

10.Consultant of Formosa Cancer Foundation 2007~2010

11.Taiwan Snriety of Blood and Marrow Transplantation 2008~present

12. Hematology Society of Republic of China (Taiwan) 2008~present

13.Consultant of Taiwan Pediatric Oncology Group 2008~2010

Award (得獎 ) Year

1. The prize of the fine pape-12th International Conference on Oral Chelation in the Treatment of Thalassaemia and Other Diseases(In Greece).

2002

2. The fine doctor of China Medical University Hospital.

2003

3. The fine doctor of China Medical University Hospital.

2004

4. Youth outstanding paper prize of the Hematology Society of Taiwan..

2006

Publication (論文發表 )


Normal values of inner canthal distance, interpupillary distance and palpebral fissure length in normal Chinese children in Taiwan.

Acta Paediatr Taiwan. 2000; 41: 22-7.

Virus infection in pediatric patients with congenital anemia in Taiwan: detection of erythrovirus B19, Epstein-Barr virus and cytomegalovirus.

Mid Taiwan J Med. 2000; 5: 207-15.

Hydrops foetalis caused by anti-Mur in first pregnancy--a case report.

Transfus Med. 2002; 12: 325-7.

Monosomy of chromosome 10q26 with mild psychomotor retardation: report of one case.


Haemolytic disease of the newborn due to maternal irregular antibodies in Chinese population in Taiwan.

Transfus Med. 2003; 13: 311-4.

Growth hormone (GH) deficiency in patients with -thalassemia major and the efficacy of recombinant GH treatment.

Ann Hematol. 2003; 82: 637-40.



Combined therapy with deferiprone and desferrioxamine successfully regresses severe heart failure in patients with beta-thalassemia major.

Ann Hematol. 2004; 83: 471-3.

Tricuspid regurgitation in patients with beta-thalassemia major.

Ann Hematol. 2004; 83: 779-83.

Interleukin 4, interleukin 6 and interleukin 10 polymorphisms in children with acute and chronic immune thrombocytopenic purpura.

Br J Haematol. 2005; 128: 849-52.

Hb Tak: a beta chain elongation at the end of the beta chain, in a Taiwanese.

Hemoglobin. 2005; 29: 65-7.

Amino acid concentrations in cerebrospinal fluid in children with acute lymphoblastic leukemia undergoing chemotherapy.

Eur J Cancer. 2005; 41: 1158-63.

Deferiprone or deferoxamine vs. combination therapy in patients with beta-thalassemia major: a case study in Taiwan.

Hemoglobin. 2006; 30: 125-30.


Essay(論文名稱 ) Journal (刊名 )Date (出版日

期 )

No association between TAP1 DpnII polymorphism and bronchopulmonary dysplasia.


Effect of deferiprone on liver iron overload and fibrosis in hepatitis C virus infected thalassemmia.

Hemoglobin. 2006; 30: 209-14.

Liver fibrosis and iron levels during long-term treatment of thalassemia major patients with deferiprone.

Hemoglobin. 2006; 30: 215-8.

Regression of myocardial dysfunction after switching from deferoxamine to deferiprone in -thalassemia major patients.

Hemoglobin. 2006; 30: 229-38.

Glutathione S-transferase M1 gene polymorphisms are associated with cardiac iron deposition in patients with -thalassemia major.

Hemoglobin. 2006; 30: 251-6.

Comparison of oral and subcutaneous iron chelation therapy in prevention of major endocrinopathies in -thalassemia major patients.

Hemoglobin. 2006; 30: 257-62.


Essay(論文名稱 ) Journal (刊名 )Date (出版日

期 )

Pilot Study on “Quality of Life” as Reflected by Psychosocial Adjustment of Children with Thalassemia Major Undergoing Iron-Chelating Treatment in West Taiwan.

Hemoglobin. 2006; 30: 291-9.

Primary B cell non-Hodgkin’s lymphoma of the penis in a child.

J Pediatr Hematol Oncol. 2006; 28: 479-80.

Fulminant pulmonary hemorrhage as the initial manifestation in systemic lupus erythematosus: a case report and review of the literature.

J Pediatric Pulmonology. 2006; 5: 81-8.

Interleukin 1β Exon 5 and interleukin 1 receptor antagonist in children with acute and chronic immune thrombocytopenic purpura.

J Pediatr Hematol Oncol. 2007; 29: 305-8.

Improvement of cardiac function in thalassemia patients using deferiprone.

Tzu Chi Med J. 2007; 19: 192-9.

Effects of chelation therapy on cardiac function improvement in thalassemia patients: literature review and the Taiwanese experience.

Hemoglobin. 2008; 32: 49-62.



Mechanisms of and obstacles to iron cardiomyopathy in thalassemia.

Frontiers in Bioscience. 2008; 13: 5975-87.

Octreotide therapy in asparaginase-associated pancreatitis in childhood acute lymphoblastic leukemia.

Pediatr Blood Cancer 2008; 51(6): 824-8.(SCI)

Granulocytic sarcoma of the colon in a child with acute myeloid leukemia presenting as hematochezia.

J Pediatr Hematol Oncol. 2008; 30(12): 981-3.

Introduction to Stem Cell Research Lab. and Tissue

Bank As the hospital pertaining to the China Medical University (CMU), the China

Medical University Hospital (CMUH) is committed to providing excellent medical services, clinical education and research. Opened for service in November 1980, there were only 200 beds in the beginning of operations. With only 20 years, passing through the initial hard time, CMUH has developed into 700-bed regional teaching hospital in 1988 and promoted into over 1700 bed medical center in 2000.

High dose chemotherapy and chemoradiotherapy followed by hematopoietic stem cell transplantation (HSCT) is an important treatment for leukaemia, lymohoma, as well as some other solid tumors. Started in 1997, Dr. Chang-Fang Chiu organized the 3-member team for the preparation of HSCT in CMUH. Dr. Chiu built the first BMT room in CMUH and educated the senior nurses in the oncology ward to take care the patients of HSCT. Mrs. Chiao-Lin Lin and Mr. Wen-Jyi Lo, senior medical technicians in blood bank, were sent to Veteran General Hospital-Taipei to learn the technique of leukapheresis, cryopreservation of stem cell, and flow cytometry. With these initial effort, CMUH conducted the first autologous peripheral blood stem cell transplantation in May 1998 and the first allogeneic peripheral blood stem cell transplantation in Nov. 1998. Dr. Su-Peng Yeh joined CMUH and led the team in Dec. 1998. Because of his active leadership and the well-integrated team work, CMUH become the major referral center for HSCT in Taiwan in the recent years.

Introduction to Stem Cell Research Lab. and Tissue

Bank Attaching to the hospital’s central vision in “to become the world’s top education and

medical center of Chinese and Western Medicine”, we will continue to offer excellent and holistic medical services as well as enhance the educational and research functions of a university hospital. To cope with the increased need of both clinical service and research in the field of stem cell transplantation and cancer treatment, a new intramural unit under the Department of Medical Research – the Stem Cell Research Laboratory and Tissue Bank (SCRL-TB) was established in Jun 2002 and Professor Chi-Long Chen was the first director. The central goal of SCRL-TB is to promote HSCT clinical service, stem cell-related clinical research, and to cryopreserve tissue/blood/body fluid in good quality to facilitate medical research. In addition to Professor Chen and the initial core members of HSCT team (Dr. Yeh, Mrs. Lin, and Mr. Lo), Dr. Kang-Hsi Wu, a pediatric haematologist, and Mrs. Liu Ya-Lin and Lai Yu-Hsiu joined the SCRL-TB in 2002,2006 and 2007 respectively. Professor Chang JG, a well-known physician scientist in the field of molecular biology, brought in the new concept and technique and became the 2nd director of SCRL-TB in 2005. Dr. Su-Peng Yeh, who led the transplantation team in CMUH, took over the place of Professor Chang as the 3rd director of SCRL-TB in 2007. Professor Chen, Professor Chang, as well as Professor Chang-Fang Chiu, continue to be a valuable advisor to SCRL-TB. With the uninterrupted effort, SCRL-TB is now a 1938 square-foot laboratory located at the 5th floor of the First Medical Building of CMUH and equipped with cell separator, cell sorter, flow cytometry, computer-programmed cooler, and various incubators and liquid nitrogen tanks. In addition to its own research, SCRL-TB also collaborates extensively with other researchers/laboratory in the field of stem cell research, such as Center of Molecular Medicine of CMUH. We welcome any domestic or international collaboration in the clinical or basic research of stem cell and stem cell transplantation. To know more about SCRL-TB or have any proposal of collaboration, please contact Dr. Su-Peng Yeh at [email protected] or Mr. Wen-Jyi Lo at [email protected].

Major Clinical Achievement of Stem Cell Research lab.

(in cooperation with adult haematologist and pediatric haematologist)

Augologous stem cell transplantation: 71 cases

Allogeneic stem cell transplantation: 141 cases

Umbilical cord blood transplantation: 7 cases Non-myeloablative stem cell transplantation:

8 cases CD34+ positive selection autologous stem

cell transplantation: 4 cases Unrelated donor stem cell transplantation: 39

cases

Specimens Cryopreserved at Tissue Bank

YearTissu

e cases

Tube number of tissue cryopreserved

Case number of blood cryopreserved

Tumor

Non-Tumor

Junction

OCTSeru

mPlasma

Buffy coat

2003 377 2442 1919 161 266 271 318 302

2004 762 2383 2126 384 1183 887 898 904

2005 687 2047 1761 231 902 820 847 860

2006 742 2107 1850 320 637 1254 1289 1293

2007 1029 2927 2475 412 352 1744 1777 1772

2008 1142 2892 2781 615 12 1493 1523 1510

2009 581 1308 1163 245 0 645 672 668

Total 5320 16106 14075 2368 3352 7114 7324 7309

Compile statistics to May 31, 2009

Overview and Research Interests

Clinical hematopoietic stem cell transplantation: infectious complication, viral hepatitis activation after transplantation, and long-term complications

Transplant immunology: the role of T helper 1, T helper2 and related cytokines in the course of graft-versus-host disease

Bone marrow-derived mesenchymal stem cell in the pathogenesis and drug resistance in leukaemia and myeloma

Bone marrow-derived mesenchymal stem cell as vehicle of gene therapy for cancer treatment

Leukemic stem cell isolation and characterization

Research Grant in SCRL-TB

2003: The role of Th1/Th2 polarization in the pathogenesis of graft-versus-host disease. (NSC-92-2314-B-039-004)

2004: The role of inflammatory cytokines in the pathogenetic mechanism of BCNU-mediated pulmonary fibrosis. (NSC-93-2314-B-039-008).

2005: Predictive value of gene expression profile of bone marrow mesenchymal stem cells determined by cDNA microarray in patients of myeloma at different stage and different clinical condition. (NSC-94-2314-B-039-022)

2006: To investigage the pathophysiological role of bone marrow-derived mesenchymal stem cell in human hematological malignancies.


2007: Generation of large-scale human red blood cells from cord blood co-cultured with mesenchymalstem cells derived from umbilical cord

2007-2009: Signal pathways and epigenetic regulation of stem cells- Human Mesenchymal Stem Cell Harvest Core (NSC 96-3111-B-039 –001)

2007: Transactivation of vimentin by beta-catenin regulates migration and invasion capacity of human mesenchymal stem cells (DMR-97-118)

2007: Dynamic change of stem cell niche adhesion molecules in patient receiving Stem cell transplantation or chemotherapy. (DMR-97-019)

2007: Animal Study of Gene Therapy Using Cancer-Specific Promoter For Breast Cancer (DMR-97-018)

2007: The difference in malignant transformation of mesenchymal stem cell in colorectal cancerous part and near-by normal part (DMR-97-020)


2008: Analysis of CD5+ diffuse large B cell lymphoma cell in immunologic tolerance and anti-apoptotic character (DMR-98-123)

2008: A Pilot Study of The Assessment of Esophageal Cancer Patients' Burden of Illness (DMR-98-009)

2008: A Pilot Study of The Assessment of Hematopoietic Stem Cell Transplantation Patients' Nutritional and Bio-physical Status (DMR-98-076)

Equipment of SCRL-TB






Published papers of SCRL-TB

Tsai MH, Chow KC, Lin TY, Yeh SP, Hsueh CT, Chi KH; 2002, 9(2): Expression of Fas ligand in patients with evident skull base involvement of nasopharyngeal carcinoma. Oncol Rep, :247-51.

Su-Peng Yeh, Ming-Tsong Yu, Kuan-Chih Chow, Ling-Ya Lai, Chang-Fang Chiu; 2002, 139: Novel clonal t(14;22)(q13;q13), der(8)(8;14)(p10;q10), del(9)(q22.3) in a patient with fulminant adult T-cell leukemia/lymphoma; Cancer Genetic and Cytogenetic, p34-37

Su-Peng Yeh; Chang-Fang Chiu; Wen-Jyi Lo; Chiao-Ling Lin; Chung-Tsen Hsueh; Yu-Mine Liao; Ying-Chun Shen; 2003; 82(1): Low Infectious Morbidity in Patients of Heavily Pretreated Hematological Malignancies Receiving Autologous Peripheral Blood Stem Cell Transplantation Without Antimicrobials Prophylaxis; Annals of Hematology, p24-29

Su-Peng Yeh, Chang-Fang Chiu, Chien-Chung Lee, Ching-Tien Peng, Chih-Yu Kuan, Kuan-Chih Chow; 2004; 126(3); Evidence of parvovirus B19 infection in patients of preeclampsia or eclampsia with dyserythropietic anemia; British Journal of Haematology, p428-33

Ying-Chun Shena, Chang-Fang Chiua,e, Kuan-Chih Chowb, Chi-Long Chenc,d, Yu-Ching Liaw d, Su-Peng Yeha,d,e (corresponding author); 2004, 34: Fatal pulmonary fibrosis associated with BCNU: the relative role of platelet-derived growth factor-B, insulin-like growth factor I, transforming growth factor-(1, and cyclooxygenase–2; Bone Marrow Transplant, p609-614


Su-Peng Yeh, Yu-Mine Liao, Chang-Hu Hsu, Chi-Long Chen, Ying-Chun Shen, Chung-Tsen Hsueh, Hsin-Hui Huang, Chang-Fang Chiu. 2004; 122(6); Gastric bleeding due to graft-versus-host disease: discrepancy between endoscopic and histologic assessment. American Journal of Clinical Pathology, p919-925.

Su-Peng Yeh, Chang-Fang Chiu, Yu-Min Liao, Wen-Jyi Lo, Yu-Ching Liaw, Chiao-Lin Lin, Jan-Gowth Chang, Chung-Tsen Hsueh. 2004; 104(11); Serum level of IL-10 correlates well with the activity of chronic graft-versus-host disease and the responsiveness to corticosteroid treatment. Blood, 458a.

S-P Yeh, J-G Chang, C-L Lin, W-J lo, C-C Lee, C-Y Lin, C-F Chiu. 2005; 19 (8): Mesenchymal stem cells can be easily isolated from bone marrow of patients with various hematological malignancies but the surface antigens expression may be changed after prolonged ex vivo culture. Leukemia [letter]: 1505-1507

Su-Peng Yeh, Chang-Fang Chiu, Yu-Min Liao, et al. 2005; 106(11); Abnormal bone marrow derived mesenchymal stem cell: another clone in hematological malignancy? Blood, 404a.(SCI, abstract)

Yeh SP, CF Chiu, WJ Lo, et al. 2006; 20; Induction of CD45 expression of bone marrow-derived mesenchymal stem cells. Leukemia (SCI, research letter): 894-896


S-P Yeh, C-F Chiu, W-J Lo, C-L Lin, Y-M Liao, C-C Lee. 2006; supplement (1); Cytokines and T cell subsets change in patients having graft-versus-host disease. Hematologica: a0309 [SCI, abstract]

Li-Yuan Bai, Chang-Fang Chiu, Yu-Mine Liao, Su-Peng Yeh, Chen-Yuan Lin, and Hsin-Hui Huang. 2007 Feb 20;25(6) Recurrent Lymphoma Presenting as Brachial Plexus Neuropathy. J Clin Oncol., p726-8 (SCI, Letter)

Lin CY, Yeh SP, Huang HH, Liao YM, Chiu CF. 2006; 85 (8): Perianal tuberculosis during neutropenia: a rare case report and review of literature. Ann Hematol, p547-548 (SCI, letter)

Chung LW, Yeh SP, Hsieh CY, Chiu CF. 2007, 86(5). Thrombotic thrombocytopenic purpura secondary to mifepristone in a patient of medical termination in early pregnancy. Ann Hematoll, p385-6 [SCI, letter]

Su-Peng Yeh, et al. accepted for publication 2009 June. Iron Chelation Therapy for Myelodysplastic Syndrome. Hemoglobin

International Conference Reports of SCRL-TB

2004 Annual meeting of American Society of Hematology; San Diego, Su-Peng Yeh, Chang-Fang Chiu, Yu-Min Liao, et al. Serum level of IL-10 correlates well with the activity of chronic graft-versus-host disease and the responsiveness to corticosteroid treatment. Dec. 4-7/2004

2005 Annual meeting of American Society of Hematology; Atlanta, Su-Peng Yeh, Chang-Fang Chiu, Yu-Min Liao, et al. ; Abnormal bone marrow derived mesenchymal stem cell: another clone in hematological malignancy? Dec. 10-13/2005

2006 Annual meeting of European Society of Hematology; Amsterdan, S-P Yeh, C-F Chiu, W-J Lo, C-L Lin, Y-M Liao, C-C Lee. ; Cytokines and T cell subsets change in patients having graft-versus-host disease. June 2006

International Conference Reports of SCRL-TB

2009 Annual meeting of European Group of Blood and Marrow Transplantation; Gothenburg, Sweden,. Su-Peng Yeh, Chang-Fang Chiu, Yu-Min Liao, Wen-Jyi Lo, Chiao-Lin Lin, Chen-Chun Lee. The Frequencies of Th1, Th1 in CD4+ T-Cells and Plasma Interkeulin-10 Are Good Biomarkers of Graft-Versus-Host Disease. March 2009

2009 Chinese Blood & Marrow Transplantation Forum in Hong Kong. Su-Peng Yeh: Effect of TBI on MSC: great concern for clinical application, Feb. 27, 2009

2009 The 2nd Pan Pacific Symposium on Stem Cells Research. Su-Peng Yeh: Clinical Application of Bone Marrow-Derived Mesenchymal Stem Cell: What I Have Learned From Patients of Hematological Malignancies, April 11, 2009

Health & Medicine

投影片 1