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presents
Functional properties of Thermostable lactic acid-
forming bacteria (B. coagulans)
Dr. Maryam Tajabadi Ebrahimi
Iranian Society of Probiotic and
Functional Food
1014 micro-organisms, >400 differentes species
stomach
Jejunum
Ileum
Colon with appendix
Duodenum
102 to 103
<105
104 to 107
109 to 1012
Lactobacilli
Streptococci
Lactobacilli
Enterobacteria
Enterococcus
Faesalis
Bacteroides
Bifidobacteria
Peptococcus
Raminococcus
Clostridia
Lactobacilli 3 Takgene BC
Takgene.com
2. Moreover, we also consume
antibacterial substances ranging
from vinegar to antibiotics
1. The changing food habits and
lifestyle force us to take processed and
sterile food, which affects our access
to, and colonization, by certain type of
bacteria.
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“Death sits in the bowels; a bad digestion is the root of all evil” - Hippocrates, ca. 400 BC
Takgene BC Takgene.com
First described by Metchnikoff in 1908“Live microbial food ingredients that alter the microflora and confer health benefit”
WHO definition(2001): “Live micro-organisms which, when administered in adequate amounts, confer a health benefit on the host”
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Probiotic
Prebiotic
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Awareness of the terms are moving into "mainstream"… with 3 of 5 consumers now aware of the term probiotics
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Dairy 38%
Bakery&cereal 23%
Beverage 12%
Meat, Fish&egg 7%
Soya Products 6%
Fat& Oil 8%
Other 6%
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L. sporogenes (Bacillus coagulance)
Contain a large number of viable lactobacilli that retain viability
during preparation in pharmaceutical dosage forms and during
storage before consumption.
The spores are thermostable as against viable L. acidophilus cells which may not withstand spray drying.
Survive in gastric secretions and bile of the upper digestive
tract and reach the intestine safely.
Settle in the digestive tract and produce enough lactic acid and
other antagonistic substances to inhibit the growth of
pathogenic bacteria.
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Takg
ene
BC
Takg
ene.
com
Bacillus coagulans has been added by
the EFSA to their Qualified
Presumption of Safety list approved
for as GRAS by the U.S. Food and
Drug Administration's Center
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ene
BC
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ene.
com
• Maturation of mononuclear phagocytes toward both
macrophage and dendritic cell
• T helper cell differentiation
• Generating T regulatory cells
• Cell wall & metabolites modulate inflammatory
processes
• Induction of IL-10,
• Possible treatment of inflammatory bowel diseases
• Adjunct therapy for relieving symptoms of rheumatoid
arthrite
Bebsons et al. World J Gastroenterol 2012 April 28; 18(16): 1875-1883
Mandel et al. BMC Complementary and Alternative Medicine 2010, 10:1
Jensen et al. BMC Immunology 2010, 11:15
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• Improve bowel movement
• Defecation frequency
• Increase number of intestinal bifdobacteria
• Decrease intestinal clostridium perfringens
• Improvements in GSRS abdominal pain
• Concentrations of intestinal ammonia, indole and p-cresol
decreased
• Improved abdominal pain and bloating in patients with
IBS
Phalguni Dutta et al. Tropical Medicine and International Health
Kal man et al., BMC Gastroenterology 2009
Larysa Hun et al., Postgraduate Medicine, 2009
Katsutoshi Ara, et al., 2002, Microbial Ecology in Health and Disease
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• Decrease in serum insulin levels
• hs-crp,
• Uric acid
• Reduces levels of lipid profile
• Increase of glutathione
• Increase of TAC Z. Asemi et al., Clinical Nutrition, 2013 Z. Asemi et al., Lipid, 2013 Shakeri et al., Lipid, 2014 Tajabadi Ebrahimi et al., 2014, Ann Nutr Metab
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• Screening for Aflatoxin binding
• Alanin transferase (ALT)
• Aspartat aminotransferase (AST)
• Alkalin transferase (ALP)
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Control Aflatoxin B1 Aflatoxin B1+ B. Coagulance
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• Take care of microbiota
• Probiotic can improve healthy
microbiota
• B. coagulance is heat resistant
probiotic
- Immune modulation
- Metabolic status in diabetic patients
- Gut health
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