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Water, p
o d0
PDX 150 mg/d, p
o d0
Water, p
o d14
PDX 150 mg/d, p
o d140
2
4
6
8
Tota
llip
ids
(g/1
00g)
Water, p
o d14
PDX 150 mg/d, p
o d140
1
2
3
4
LDLr
mR
NA
/RPL
P0m
RN
A
Putaala H.1, Raza G. S.2, Tiihonen K.1, Mäkelä K. A. 2, Herzig K. H.2
BACKGROUND
METHODS
RESULTS
DISCUSSION
HYPOLIPIDEMIC EFFECTSOF POLYDEXTROSE IN MOUSE
1DuPont Nutrition & HealthActive NutritionSokeritehtaantie 2002460 KANTVIK, FINLANDE-mail: [email protected]
The information contained herein is based on data known to DuPont or its affiliates at the time of preparation of the information and believed by them to be reliable. This is business-to-business information intended for food, beverage and supplement producers, and is not intended for the final consumer of a finished food, beverage or supplement product. The information is provided “as is” and its use is at the recipient’s sole discretion and risk. It is the recipient’s sole responsibility to determine the suitability and legality of its proposed use of DuPont products for its specific purposes. Information and statements herein shall not be construed as licenses to practice, or recommendations to infringe, any patents or other intellectual property rights of DuPont or others. DUPONT HEREBY EXPRESSLY DISCLAIMS (I) ANY AND ALL LIABILITY IN CONNECTION WITH SUCH INFORMATION, INCLUDING, BUT NOT LIMITED TO, ANY LIABILITY RELATING TO THE ACCURACY, COMPLETENESS, OR USEFULNESS OF SUCH INFORMATION, AND (II) ANY AND ALL REPRESENTATIONS OR WARRANTIES, EXPRESS OR IMPLIED, WITH RESPECT TO SUCH INFORMATION, OR ANY PART THEREOF, INCLUDING ALL REPRESENTATIONS AND WARRANTIES OF TITLE, NONINFRINGEMENT OF COPYRIGHT OR PATENT RIGHTS OF OTHERS, MERCHANTABILITY, FITNESS OR SUITABILITY FOR ANY PURPOSE, AND WARRANTIES ARISING BY LAW, STATUTE, USAGE OF TRADE OR COURSE OF DEALING.
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Hyperlipidemia is a risk factor for cardiovascular and metabolic diseases. It is established that elevated plasma triglyceride-rich lipoproteins and low levels of HDL-cholesterol levels are directly related to the risk of cardiovascular disease [1].
Polydextrose (PDX, Litesse® Ultra™, DuPont) is soluble glucose polymer used as a fat and sugar replacer as well as dietary fibre. As PDX has potential to improve hyperlipidemia [2-4] and satiety [5-7] we investigated effects of PDX on lipid profiles in mice fed with fiber-deficient and western diet with an attempt to further characterize lipid-attenuating mechanisms.
Male C57BL/6 (n=36) mice were fed either fiber- deficient (<0.5% fibre 10% kcal fat) or western- diet (5% fiber, 41% kcal fat) for 2 weeks. Animals were gavaged twice a day either with water containing PDX 150 mg/day or water alone as control. Body weight, food intake and indirect calorimetric measure-ments were performed in a home cage- based monitoring system (LabMaster®). Fasting blood samples and feces were collected for lipid measurements. Gene expression for lipid metabolism was performed by real–time PCR in liver.
In a recent meta-analysis, it was concluded that PDX can reduce voluntary energy intake at a subsequent meal [5]. The present study shows that longer-term consumption of PDX might translate into a reduction of cumulative food intake and loss in body weight. Polydextrose has been shown to decrease postpran-dial elevation of triglycerides [2]. The present study shows a longer-term effect of PDX on triglycerides and total cholesterol reduction, and is in accordance with previous studies [3]. The upregulation of LDLreceptor gene in liver could increase LDL clearance by the liver which would then translate into reduction of total cholesterol and triglycerides [1]. The binding of lipids to fiber and evacuation through feces may be additional mechanism.
2Institute of Biomedicine,Dept.of Physiology and Biocenter of Oulu,Univ. of Oulu,Medical Research Center Oulu and Oulu Univ. Hospital,OULU, FINLAND
References:[1] Miller, M., et al., Circulation, 2011. 123(20): p. 2292-2333. [2] Tiihonen, K., et al., Nutrition Journal, 2015. 14(1): p. 23. [3] Pronczuk, A. and K.C. Hayes, Nutrition Research, 2006. 26(1): p. 27-31. [4] Schwab, U., et al., European Journal of Clinical Nutrition, 2006. 60(9): p. 1073-80. [5] Ibarra, A., et al., Appetite, 2014. 87C: p. 30-37. [6] Hull, S., et al., Appetite, 2012. 59(3): p. 706-712. [7] Ranawana, V., A. Muller, and C.J. Henry, European Journal of Nutri-tion, 2013. 52(3): p. 885-93.
Cumulative body weight gain
Days
Cum
ulat
ive
body
wei
ght(
g)
0 2 4 6 8 10 12 140.0
0.5
1.0
1.5
2.0
2.5
Water, poPDX 150mg/day, po
Cumulative food intake
Days
Food
inta
ke/b
ody
wei
ght[
g/g]
0 2 4 6 8 10 12 140.0
0.6
1.2
1.8
water, popdx 150mg/day, po
p<0,001
pd
Plasma triglyceride
Plas
ma
trig
lyce
ride
(mg/
dL)
Water, p
o
x 150m
g/day, p
o20
40
60
80
100
p<0,0005
pdx 1
Plasma cholesterol
Plas
ma
chol
este
rol(
mg/
dL)
Water, p
o
50mg/day
, po
100
120
140
160
180
p<0,019
Plasma triglyceride
Plas
ma
trig
lyce
ride
(mg/
dL)
Water, p
o
pdx 150m
g/day, p
o0
20
40
60
80
100Plasma cholesterol
Plas
ma
chol
este
rol(
mg/
dL)
Water, p
o
pdx 150m
g/day, p
o0
50
100
150
200
p<0,037
Figure 1. When fed with fiber-deficient diet, PDX reduced fasting plasma cholesterol (p<0.05) (A), while the reduction in plasma triglycerides was not significant (B).
A B
Figure 2. When fed with western- diet, PDX significantly reduced food intake (p<0.001) (A), which translated to a trend in body weight loss (p=0.074) (B). Both fasting plasma cholesterol (p<0.05) (C) as well as triglycerides (p<0.001) (D) showed reduced values compared to control.
Figure 3. When fed with western- diet, the total fecal output of lipid increased numeri-cally with PDX (A) and there was a trend for upregulation of LDLr gene expression in liver by PDX (B).
A B
C D
A B
