What’s in the Water?

Particle Characterization using AFM/IR Topographic and Chemical Information

Mark Banaszak Holl January 2016

Anasys NanoIR2

Height Deflection Optical Microscopy

Differentiating Composition PMMA Beads in Epoxy

AFM Images

IR Spectra PMMA

Epoxy

IR Imaging

1512 cm-1

epoxy 1730 cm-1

PMMA Ratio

Polystyrene (0.5 µm ) and Melamine (1 µm ) Beads

Height (tapping)

Height (tapping)

Mel

amin

e

Pol

ysty

rene

AFM-IR on Lake Water Sample Rachel Merzel and Rachel Cable

Sample from Duhaime Lab

Sample: 20-100 nm fraction Cloudy, yellow-brown supernatant sediment at the bottom

Optical image

AFM topography image

Silica!

Stiffness map