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IMPACT OF FOOD HABIT ON BODY ELEMENTS LEVEL AND HEALTH

 

 Dr. G. NAGARAJ

Lab. Asst. in Zoology  

    

 ZOOLOGY SECTIONREGIONAL INSTITUTE OF EDUCATION (NCERT)

MYSORE e-mail: gnriem@gmail.com

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Background • Every food item is a source for multi-

element exposure

• Food habit may have association with changes in

body element levels

• Such altered status of body elements may leads to

health disorders

• It is needed to assess concentration of elements in

subjects with chronic health hazard

• Such assessment is done by biological monitoring

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Objectives of the study

To find out the influence of food habit on body levels of trace elements and in turn it’s effect on physical health of Mysore subjects.

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MATERIALS AND METHODS

1. Subjects: The residents of Mysore city & surrounding villages (M:100 & F:76)

2. Samples: Teeth, blood, urine, scalp hair & fingernail3. Sampling places: Teeth: JSS dental college, Mysore Blood, scalp hair, fingernails and urine: Kamakshi,

Bassappa Memorial and Vickram Hospitals, Mysore.

4. Questionnaire: personal and sample characters were collected

5. Processing of samples:Washing: T, F - DDW, H, N, - DDW & Acetone-wit magnetic stirrerDrying: Hot air oven - 50C - 24hrDigestion: HNO3 & HClO4 (4:1), Kjeldhal apparatus – 80’C - 8 hr Make up to 10 ml with DDW and stored in refrigerator at 4’C

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Element Analysis:

Sample Element Instrument Place

TeethCr, Cu, Fe, Ni, Pb, Zn

AAS 6 VARIO

AAS-GBC 902

CES- Anna Univ, Chennai.

SES-JNU- New Delhi.

Blood, urine, hair,

fingernail,

Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, Zn

ICP-AES - JY-IYON-2002–2 DEE - SJCE,

MysoreQuality control studies: Certified Reference Material (CRM) i.e. human hair powder supplied by the National Institute of Environmental studies (NIES), Japan, (NIES-CRM) was analysed Anna University, Chennai Jawaharlal Nehru University (JNU), New Delhi.

Statistical analysis: Mean, Standard Deviation, Student ‘t’ test.

Mean elements concentration for food habit and blood sugar level were computed in each sample & given in following tables;

ResultTable 1. Comparison of element levels of blood

Note: Significance: * -P 0.05, N – Number of samples, SD- Standard Deviation.

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Table 2. Comparison of element levels of teeth

Note: Significance: * -P 0.05, N – Number of samples, SD- Standard Deviation

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Table 3. Comparison of element levels of urine

Note: Significance: **-P 0.01, N – Number of samples, SD- Standard Deviation.

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Table 4. Comparison of element levels of scalp hair

Note: Significance: * -P 0.05, N – Number of samples, SD- Standard Deviation

Table 5. Comparison of element levels of fingernail

Note: Significance: **-P 0.01, N – Number of samples, SD- Standard Deviation.

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FINDINGS

1.Rural vegetarian had lower Cd-B & Pb-B than non-veg.

2.Rural diabetics had higher Co-H & Fe-H than non-diabetics.

3.Urban diabetics showed lower Zn-FN, & higher Cd-FN,

Cr-FN, Cu-FN, Mn-FN, Ni-FN, Pb-FN & Pb-T.

4.Lower Zn & Higher Pb are due to food habit & environment;

is one of the casual factors related to diabetes.

5.Positive correlation exists between elements & diabetes.

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CONCLUSION 

Food habit affects/changes the elements level and causes elements imbalance leading to altered element metabolism, which may be one of the causal factor for diabetes.

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THANK YOU