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MORPHOLOGICAL CHERACTERIZATION OF MICRO-ALGAE AND CYNOBACTERIA

PRESENTING BYSunirmal DasB.Tech ,Final yearBio engineering

Under the guidance of Dr. Tridib Kumar BhowmickCo-guideDr. Kalyan Gayen

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Micro-algae and Cynobacteria

• Found in freshwater and marine systems living in both the water column and sediment.

• They are unicellular species which exist individually, or in chains or groups.

• Microalgae, capable of performing photosynthesis, are important for life on earth.

• Bio diversity of microalgae is enormous estimated that about 200,000-800,000 species in many different genera exist of which about 50,000 species are described.

• Algae can be use as Fuel source (biodiesel,bioethanol,biobutanol), food supplement, Stabilizing agent, Fertilizer.

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• PERMANENT SLIDE PREPARATION OF MICRO-ALGAE AND CYNOBACTERIA.

• MORPHOLOGICAL CHERACTERIZATION OF MICRO-ALGAE AND CYNOBACTERIA.

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Permanent slide preparation

What...??

A permanent slide is a slide can be fixed and locked into place. While temporary slides usually have a top and a bottom with the object secured in-between, permanent slides have a top and bottom that are glued or sealed together for longer time.

Why…??

• Permanent slides have the advantage of being vacuum-sealed within frame to prevent movement or leakage.

• Ideal for transport or repeated presentational viewings.

• With permanent slides we can preserve the specimen for longer than temporary slide.

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Permanent slide preparation

How…??

Materials & equipment required1. Fresh water.2. Sample of algae.3. Formaldehyde(40%).4. Filter paper.5. Slide.6. Cover slip.7. Oven.8. Microscope.9. Glycerine jelly.

Referance: Text book of algae by O.P.Sharma , passage no 6.5.1, laboratory culture and staining page no:71

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Permanent slide preparation

Procedure

1. Wash the slide and cover slip properly so that there is no particles .2. Put a drop of fresh water on slide.3. Wash the algae with PBS 10m for 2 times.4. Place the algae on the slide .5. Add a drop of formalin (40%).6. Use filter paper to drain away excess liquid.7. Put some glycerine on algae.8. Keep the slide on oven for 2-3 minutes at 60°c.9. Put washed cover slip on it.10. Again keep the slide in oven for 2-3 minutes .11. Remove the excess glecerine .12. Seal it properly with glu or nail polish .

Referance: Text book of algae by O.P.Sharma , passage no 6.5.1, laboratory culture and staining page no:71

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Morphology Characterization

What…??

Morphological characterization is identification of species and structural analysis of it . Task is to identify the species from different classes of micro-algae and cynobacteria.

How…??

Stage 1

Preparation of good permanent slide.

Stage 2

Take good sample photos using microscope & camera.

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Morphology Characterization

Stage 3

Identification and morphology characterization.

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Month Week Task Status

Dec 1st week Mid term 2nd week Literature morphology 3rd week Literature morphology 4th week Literature morphology

Jan 1st week Literature morphology 2nd week Basic operations of lab 3rd week Basic operations of lab 4th week Basic operations of lab

Feb 1st week Preparation of slide & microscopic photos

2nd week Preparation of slide & microscopic photos

3rd week Preparation of slide & microscopic photos

4th week Preparation of slide & microscopic photos

Mar 1st week Morphological study 2nd week Morphological study 3rd week Morphological study 4th week Mid term

Apr 1st week Final report preparation 2nd week Final report preparation 3rd week Final presentation

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Thank you