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High Performance Liquid Chromatography
High Performance Liquid ChromatographyGiven By : Syed Murtaza Shah Roll no : 16CH48
SUBTOPICS :Chromatography DefinitionHistory of ChromatographyWorking Principle of ChromatographyTypes of ChromatographyHigh Performance Liquid Chromatography(HPLC)Working, Parts and Applications of HPLC SYSTEM
How does it Works??
Simple Experiment to understand Chromatography
History of Chromatography
Experiment of M.S Tswett
Basic Termologies used in ChromatographyTermDefinitionMobile phase or carriersolvent moving through the columnStationary phase or adsorbentsubstance that stays fixed inside the columnEluentfluid entering the columnEluatefluid exiting the column (that is collected in flasks)Elutionthe process of washing out a compound through a column using a suitable solventAnalytemixture whose individual components have to be separated and analyzed
Working Principle of Chromatography
Chromatography works on Adsorption Principle
Types of Chromatography
Paper Chromatography
Gas Chromatography
Column Chromatography
High Performance Liquid Chromatography
Parts of Hplc System
Working of HPLC system
How does the User knows what and how many types of Elements collected form sample
Scales of HPLC
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