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BIOGLYPHICSVISUAL LAB PROTOCOLS. CREATED USING A SIMPLE DRAG & DROP WEB APP.100% OPEN SOURCE. COMPATIBLE WITH LAB AUTOMATION SOFTWARE.
11 2 3 4
MIX
42 C 0 C
37ºC
A 2014 GENSPACE IGEM PROJECT
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There are 4 problems with text protocols.
1. Poor Consistency Each person wri0ng a protocol phrases things a bit differently and focuses on different content. For a beginner in the lab this can be confusing. “Spin down” vs. “Centrifuge”
2. Poor VisualizaBon It can be hard for a beginner in a lab to connect the text of a protocol with clear ac0ons they need to perform.
3. Low EfficiencyWri0ng text protocols is not very 0me efficient. You need to spend 0me deciding word choice and what level of detail to cover.
4. Machine IllegibleBecause the text of protocols is not consistent in language or formaHng, computer programs that run lab automa0on soJware are not able to use it.
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MAKING THE DREAM REAL: WE GOT THE COMMUNITY INVOLVED
We brought together graphic designers and ar0sts from all over the NYC metro area and invited them to design BIOGLYPHICS at a event hosted in a Brooklyn community work space.
On 7/7/14 we hosted a public event -‐ The BioGlyphics Iconathon.
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An Early Prototype MATERIALS
PURPOSE
1
2
B
C
3
W
A
IGEM TRANSFORMATION PROTOCOL
competentcells
IGEM distributionw/ DNA
petri dish w/ LB agar & appropriate antibiotic
2 ml tube
deionizedwater
SOCmedia
RFPcontrol
This procedure allows you to insert a BioBrick part (from the IGEM distribution kit) into cells. The IGEM distrubution kit contains an array of wells that contain plasmids. These plasmids contain the DNA for the BioBrick part. This plasmid will be inserted into the cell and then the cell will be plated on a petri dish.
BIOGLYPHICS: A GENSPACE PROJECT 7/3/14
42 C
11 2 3 4
MIX
2µL2µL50µL MIX 2µL
30 MIN 1 MIN
0 C
A1 W 2A A
A A 3
200µL
A
5 MIN
0 C
A
BBB
37ºC
SHAKE OR TUMBLE
A
2 HOURS
200µL
A C
SPREAD
C
37ºC
13 HOURSAGAR SIDE UP
C
PROCEDURE1 2 3 4 5
6 7 8 9 10
11 12 13
The complete IGEM transforma0on protocol in BIOGLYPHICS.
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Why We Like It.100% consistent.Each protocol follows the same logical format.
Easy to understand and visualize. Materials and Procedures are visually defined just like IKEA instruc0ons.
Fast & easy to create. Simple drag and drop web/android/iOS app interface.
Will be 100% machine readable for lab automaBon so^ware.Making a protocol in this format will automa0cally build machine code behind the scenes.
MATERIALS
1
2
B
C
3
W
Acompetent
cells
IGEM
distribution
w/ DNA
petri dish
w/ LB agar
& appropriate
antibiotic
2 ml tube
deionized
water
SOC
media
RFP
control
42 C
11 2 3 4
MIX
2µL2µL50µL MIX 2µL
30 MIN 1 MIN
0 C
A1 W 2A A
A A 3
200µL
A
5 MIN
0 C
A
BBB
37ºC
SHAKE OR TUMBLE
A
2 HOURS
200µL
A C
SPREAD
C
37ºC
13 HOURSAGAR SIDE UP
C
PROCEDURE1 2 3 4 5
6 7 8 9 10
11 12 13
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Our Dream is...
To create a fully featured online community and repository of visual laboratory protocols.
Imagine a Thingiverse.com or allrecipies.com for visual laboratory protocols.
Drag and drop web app for creaBng a BIOGLYPHICS protocol.Simply drag and drop the correct icons and then fill in the details.
In under 2 mins you can build a complete protocol.
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It works just like IKEA InstrucBons
STEP 1: DEFINE YOUR MATERIALS
We have a test tube of Soc Media defined as 1
STEP 2: CREATE YOUR PROCEDUREUse procedure icons and materials to create your protocol.
We have an empty 2ml Tube defined as A
We are pipeHng 5 microliters of 1 into A.
We centrifuge A for 30 sec.
We incubate & shake A for 24 hours.
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When you create a visual protocol...
The code you see here is an early a4empt at translaBng Bioglyphics input into Pr-‐Pr
The web app will automaBcally generate lab automaBon code.
BIOGLYPHICS: A 3 STAGE PROCESS OF DEVELOPMENT
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STAGE 1Proof of concept and basic early prototype. Ini0al development of Bioglyphic icons and explora0on into lab automa0on programming.
STAGE 2Raise funding and find dedicated volunteers for the full development of the web applica0on and online community.
STAGE 3Spread BioGlyphics to other community labs, teachers, and bio makers across the country.
WE ARE HERE 2015-‐2016 Late 2016