Biodegradation of Polyacrylamide by Bacteria Isolated from Sewage

Sludge

—— ZhengGuolin

Main Contents

To choose a background

Research methods

Expected results

To choose a background

Polyacrylamide(PAM)is a general term for acrylamide homopolymers and copolymers.As an important product of chemical industry with high-tech and high accessional values,polyacrylamide has been widely applied to different fields of industry and agriculture,and even to people’s daily life.

It was generally considered that polyacrylamide

was an extraordinarily stable macro mole

cularpolymer. But unfortunately, polyacrylamide

is found with slow physical degradation (heat,

cutting) , slow chemical degradation (hydrolysis ,

oxidation,catalysis oxidation) and slow

biodegradation under natural conditions,and it

finally produced different oligomers, as well as

acrylamide, which possess neurotoxicity,and are

directly and indirectly harmful to humanhealth.

as well as acrylamide, which possess

neurotoxicity, and are directly and indirectly

harmful to humanhealth. So,the studies on

degradation of polyacrylamide are greatly

inportant.However,there have been very few

studies on biodegradation of

polyacrylamide till now.

Research methods Strains of screening and separation

Active sludge many microbial contact a period of

time after just possess the inhibition of content

degradation ability, Therefore need to sludge

domestication. Will 100mL canister of sludge

added to the container with 1000 mL beaker of

based medium, In 35 thermostatic slot, use ℃aquarium inflatable can aeration.

To cultivate activation, after 3 d every 72 h

replacement 1 times and gradually increase the

mixture containing poly sewage concentration

( polyacrylamide concentration: 50 ~ 500 mg, L -

1), cultivate domesticated after 45 d bacteria

separated

With flat crossed separation by bacteria, will be placed in the flat 37 biochemical ℃incubator cultivate 48 h, then pick take different forms of colonies on flat crossed purification bacteria According to the colony morphology and microscope observation results, and repeat crossed separation, until they get the pure bacteria. Then again to the strains were on physiological and biochemical identification.

Expected Results

some strain bacteria which degraded polyacrylamide were screened from activated sludge. One of the three strains,which could degrade polyacrylamide efficiently.

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