1. Microbial Risk in a Hypothetical Indian River QMRA Workshop, Drexel university Woo Hyoung Lee, Qiaozhi li, Sharada Prasad, Matthew Verbyla1/10/141

2. Objective Stream AA (near Delhi, India) is polluted with domestic wastewater and surface runoff from Village BB. Objective: Assess microbial risks for drinking, swimming, and 1/10/14 irrigating spinach and2 3. Hazard (I): Vibrio Comma-shaped, gram-negative bacillus (1-3m(L)x 0.5-0.8m(D)) Infection: Fecal-oral transmission by food or water supply 2nd transmission: seasonal effect dense population Symptom/Disease: Diarrhea, dehydration, hypovolemic shock/death Incubation time: 4hrs-5 days (avg. 2-3 days) Mortality rate: 50% (untreated) 0.5 cases/106 population (U.S., 2003-2008)1/10/143 4. Hazard (II): Shigella Rod-shaped, gram-negative bacteria Infection: person-to-person contact , contaminated food and water 2nd transmission: mass outdoor gathering (+) vs. handwashing (-) Symptom/Disease: Diarrhea, dysentery, acute colitis Incubation time: 1-4 days Mortality rate: 99%>99%>99%99%Swimming (accidental water ingestion)Adults>99%>99%~79%~16%~2%Children>99%>99%~97%~34%~4%Working in the fieldFarmers~11%~1%