Stimulus-triggered fate conversion ofsomatic cells into pluripotency

Japanese research team discovered an unexpected phenomenon of somatic cell reprogramming into pluripotent cells

Introduction

• Stem cells : ability to develop to any kind of cells

• Can be found in body or induced in laboratory

Why stem cells ?

• Cell-based Therapy .. Replace failed systems

• Research tool .. Study development

• resource for testing new medical treatments

Sources of stem cells

• Naturally :

When cells mature from embryo , some still immature

Bone marrow , blood , adipose tissue

• Induced :

• Transplantation of nuclei

• Modification of somatic cells

• Harsh conditions . . Acid bath

What is new in this research ?

• Researcher develop a way to generate SC* from adult cells ( acid bath )

• Easy

• Fast

• No risk

* SC : stem cells

Designing the experiment

• Cell model : hematopoietic spleen (CD 45+)

• Animal model : mouse

• Conditions : acidic

• pluripotency markers : Oct 4

( Nanog, Sax2, Ecat1, Esg1, Dax1 )

No study so far has proven that such pluripotent cells can arise from

differentiated somatic cells

Results and discussion

Conclusion

This study show that when adult cells put in sub-lethal conditions it would not die ,

Instead it would converted to the un differentiated situation in which it Would

develop to any kind of cells

References

Haruko, Obokata, Wakayama Teruhiko, Kojima Koji, Vacanti Martin P, Niwa Hitoshi, Yamato Masayuki, and Vacanti Charles A. "Stimulus-triggered Fate Conversion of Somatic Cells into Pluripotency." Nature.com. Nature Publishing Group, 29 Jan. 2014. Web. 18 Mar. 2014.

Done by : Belal Abu Haneya

Hashemite University – Jordan

Supervisor : Dr. Abd Allatif Al Ghzawi

19 - 3 - 2014