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Meeting welcome and overview of Eukaryotic -Omics ressearch at UC Davis
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#SMBEeuks
April 29 – May 2, 2013UC Davis Conference Center
Logistics
• Wi-Fi• Lunch/Dinner
Logistics
• Wi-Fi• Lunch/Dinner• Instructions for posters• Instructions for talks• Sponsor thanks• Prize for travel award recipients
It all started in Florida…
2011 Catalysis Meeting at NESCent
Future Goal:
ResearchCoordinationNetwork
Building a Eukaryotic Community
• Relaxed meeting schedule• Emphasis on speaker diversity• Breakout groups on Thursday - outputs
• Get to know your neighbor – socialize!• Tweet! (Profusely, if possible)
Photo Credit: J. Baldwin, M. Mundo-Ocampo
Biodiversity?
Phylogeography?
Environmental Impacts?
Extract Environmental DNA
Amplify rRNA
High-throughput sequencing
Community analysis
Diverse marine community
EASYEASY
EASY
VERY Difficult!
http://phylosift.wordpress.com
Explicitly Phylogenetic ApproachesAligned environmentalsequences
Guide Tree
Evolutionary Placement of short reads
We provide:• Support for Paired End (raw) Illumina data• Marker gene data for Bacteria, Archaea,
Eukaryotes, Viruses• Taxonomy assignments based on probability
distributions over a reference phylogeny• Complement to existing tools – QIIME, etc.
– Inputs/outputs will be compatible for use with other software tools
Great!
Not Bad
Getting Tricky…
MarineMetagenome
Tree PlacementSing Tree - Guppy
AcknowledgementsUC Davis• Jonathan Eisen• Aaron Darling• Guillaume Jospin• Dongying Wu
_________________________________________________________________________________________________________
PhyloSift Software Development on Github:https://github.com/gjospin/PhyloSift
Google Group for user support: https://groups.google.com/d/forum/phylosift
Twitter: @PhyloSift