Upload
wagner-college
View
485
Download
5
Tags:
Embed Size (px)
DESCRIPTION
Salvatore Valenti, a graduating senior at Wagner College, used this Power Point slideshow in delivering his senior thesis presentation.
Citation preview
Isolation and Transmission Electron Microscopic Characterization of
Bacteriophages in Sewage Specific for E.coli O157:H7 and Citrobacter Freundii
Salvatore Valenti-MicrobiologyDepartment of Biological Sciences
My Question?
• Can I prove that phages are present in the sample collected from a sewage plant by visualizing them via Transmission Electron Microscopy?
• By answering this question I will help future generations of Wagner microbiology students perform further analyses of bacteriophages.
Philips CM 100 Transmission Electron Microscope
E.Coli O157:H7 phage from my sample
A B
C D
Citrobacterphage from my sample
A B
C D
Conclusion
• Water samples from sewage plants are rich in phages for E. coli O157:H7 and Citrobacter freundii as it was proven with electron microscopy.
• Having learned the techniques to isolate bacteriophages and visualize them with the electron microscope makes me more competitive in the job market.
Acknowledgements
• Dr. Kathleen Bobbitt• Prof. Christopher Corbo• Zulmaire Franco• Dr. Roy Mosher• Dr. Adam Houlihan