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Presentacion de 11th Asian Maize Conference which took place in Beijing, China from November 7 – 11, 2011.
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I would like to present the results of the study of
Mehmet Ali TURKAY 1
1East Mediterranen Agricultural Research İnstitute-
Adana/TURKEY
Development and evaluation of a maize
variety with resistance against
Pink stem borer (Sesamia nanogrioides)
And European corn borer (Ostrinia nubilalis)
There are two major insects that negatively affect maize production in Turkey.
One of them is
Pink stem borer (Sesamia nanogrioides)
and another one is European corn borer (Ostrinia nubilalis)
While both insects are found in the Mediterranean and the Aegean region, but
European corn borer can just be found in Black Sea region of Turkey
Seventy percent (%70) of planting area and ninety percent (%90) of the total
production area of maize of our country has been affected from these two
insects.
Pesticides are applied three times in second crop season and one in the first
crop season for these insects.
Pink stem borer gives five generations in the Çukurova region of Turkey, while
European corn borer has three generations
While European corn borer demages plant after the plant grow to 30-40 cm,
Pink stem borer can demage in every period of growing.
TÜRKAY CHECK
I will explain the results of the two different experiments Firstly, The results of an experiment that carried out without chemical application in
second crop conditions in 2009- 2010 Secondly, An experiment with and without chemaical applications in second crop
conditions in 2010 I will talk on the yield and evaluation of the resistance For evaluation of the resistance Tunnel length per plant method was used
CHECK TÜRKAY
CHECK
Variety Grain
Yield
(t/ha)
Gr Tünnel
Length/
Plant (cm)
Gr
25Ax10 6.0 g 3.2 h
DK 6022 5.0 h 43.2 de
P32W86 4.8 hı 53.4 ab
SİDE 4.7 hı 45.6 cd
DK 5783 4.5 hj 47.4 cd
P 3394 4.1 hj 50.4 bc
DK 626 4.0 ıj 48.7 bc
HELEN 3.6 j 50.5 bc
7C2x9A 2.7 k 56.9 a
P<0,01 CV 12.0 17.0
Table1. The Yield and Tunnel Length Values of The Pesticide-Free
Experiment That Carried Out İn Second Crop Conditions İn 2009-2010 (t/ha)
Variety Grain
Yield
(t/ha)
Gr Tünnel
Length/
Plant (cm)
Gr
TÜRKAY 10.5 a 1.0 h
22x9B 10.1 a 0.8 h
22x5A 10.0 a 1.0 h
24Ax10 9.0 b 0.4 h
9Bx24A 8.3 bc 0.2 h
5Ax25A 8.0 cd 0.8 h
KARAÇAY 7.5 ce 35.0 f
18Ax5A 7.3 df 16.2 g
GÖZDEM 7.3 df 40.3 e
9Bx25A 6.9 eg 1.2 h
22x10 6.4 fg 4.0 h
The Yield and Tunnel Length Values of The Pesticide-Free Experiment That
Carried Out İn Second Crop Conditions İn 2009- 2010 Any pesticide was not used for this study Two hundred larvas per plant infected from the natural fauna and twenty five
larvas per plant infected artifically
Nine checks that widely grown in the region, three tolerant genotype, ten
experimental hybrids and TÜRKAY variety were used as plant material
While the yield of the checks were between 3.6 and 5.0 ton/ha, the yield of the
TÜRKAY variety was 10.5 ton/ha The yield of the tolerant hybrids varied from 4.7 to 7.5 ton/ha While tunnel length per plant of the checks varied between 43.2 cm and 53.4
cm, the length inTÜRKAY variety was only 1 cm.
CHECK TÜRKAY
Table 2. Yield losses of the experiment which carried out with
pesticide and pesticide free in second crop season in 2010 (t/ha)
Pesticide-
Free
Pesticide
Variety Grain
Yield
(t/ha)
Grp Grain
Yield
(t/ha)
Grp
DK6022 3.7 ı 7.3 h
P3394 3.6 ı 7.4 fh
P32W86 3.5 ı 8.6 dh
HELEN 3.3 ı 7.0 h
DK 5783 3.3 ı 7.0 h
DK626 3.0 ı 8.0 eh
P<0,01 CV 14.0
Pesticide-
Free
Pesticide
Variety Grain
Yield
(t/ha)
Grp Grain
Yield
(t/ha)
Grp
TÜRKAY 11.0 ab 11.4 a
22X9B 10.2 ad 10.7 ac
22X5A 10.2 ad 10.9 ac
24AX10 9.1 cg 9.4 bf
5AX25A 8.2 eh 8.5 dh
9BX24A 8.0 eh 8.5 dh
KARAÇY 7.3 gh 9.6 ae
18AX5A 7.3 gh 7.7 fh
GÖZDEM 7.0 h 9.3 bf
SİDE 3.8 ı 8.0 eh
Yield losses of the experiment which carried out with pesticide and pesticide
free in second crop season in 2010
In this experiment TÜRKAY variety had 11 ton/ha in pesticide free condition,
11.4 ton/ha in pesticde applicated condition The yield of the checks in pesticide-free varied between 3 and 3.7 ton/ha The yield of the checks in pesticide applicated conditions varied between 7 and
8.6 ton/ha
RESİSTANT LİNE SUSCEPTİBLE LİNE
0
10
20
30
40
50
60
70
Table 3. Yield losses as % in the pesticide-free and pesticide
applicated experiment
Yield losses as percent (%) in the pesticide-free and pesticide applicated
conditions While yield losses in the checks varied between % 50 and % 65, just % 3 yield
loss was recorded for TÜRKAY variety
CHECK
TÜRKAY
Pesticide-
Free
Pesticide
Variety Tünnel
Length
/Plant
Gr Tünnel
Length/
Plant
Gr
DK 5783 61.2 a 6.1 hj
P32W86 58.9 ab 7.5 gı
DK6022 57.3 ab 5.1 hk
HELEN 57.2 ab 2.4 jl
P3394 54.4 bc 13.1 fg
DK626 50.5 cd 8.3 gh
SİDE 49.4 d 9.6 fh
GÖZDM 47.5 d 3.2 ıl
KARAÇY 35.6 e 9.1 fh
Pesticide-
Free
Pesticide
Variety Tünnel
Lengt/
Plant
Gr Tünnel
Length/
Plant
Gr
18AX5A 13.3 f 2.6 ıl
5AX25A 1.4 fg 0.1 l
22X5A 0.5 jl 0.0 l
9BX24A 0.4 kl 0.0 l
24AX10 0.3 kl 0.6 kl
22X9B 0.3 kl 0.0 l
TÜRKAY 0.2 kl 0.0 l
P<0,01 CV 17.0
Table 4. Pesticide applicated and Pesticide-free trial values of
tunnel length per plant (cm)
Pesticide applicated and Pesticide-free values of tunnel length per plant The demage values of the checks varied from 2.4 to 13.1 However it is supposed that these values must be zero (0) for the pesticide
apllicated areas
Because of intensive insect population, maximal % 60 of the plant protected
with three times pesticide application
TÜRKAY CHECK
As a Result
As a Result TÜRKAY variety has high yield in both pesticide-free or pesticide applicated
fields
It can be seen, TÜRKAY variety is resistant to both the European corn borer
and Pink stem borer.
TÜRKAY variety
The ear of the TÜRKAY variety
TÜRKAY CHECK
Green TÜRKAY variety and checks that affected from the insects
TÜRKAY CHECK
Standing TÜRKAY variety but broken and a lodging check
CHECKS TÜRKAY
While TÜRKAY is green, two checks that in the border of the experiment are dead early
CHECK TÜRKAY
Both checks are broken and lying, while TÜRKAY variety is standing
TÜRKAY
CHECK
A check that grown beside the TÜRKAY in another replication is dead but Turkay variety is green.
TÜRKAY CHECK
Check broken and lying but whole plants of TÜRKAY variety is standing
SUSCEPTİBLE
SUSCEPTİBLE RESİSTANT
RESİSTANT
We can see the demage of the European corn borer and Pink stem borer
TÜRKAY
There is no demage on TÜRKAY variety’s ear but demage on check’s ear can bee seen
TÜRKAY
The vascular bundle of the TÜRKAY variety
The vascular bundle of the CHECKS
TÜRKAY
CHECK
The vascular bundle of the TÜRKAY variety is healthy but vascular bundle of the checks like an
empty pipe
SUSCEPTİBLE LİNE RESİSTANT LİNE
Resistant line is the parent of the TÜRKAY variety
SUSCEPTIBLE LINE
RESISTANT LINE
SUSCEPTIBLE LINE
Movingİmge
Resistant lines is standing on the harvest time but it is not
possible to see susceptible lines because they were all dead.